Nikon ECLIPSE E400 POL Instructions Manual page 38

Polarizing microscope
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Problem
Cause
Countermeasure
The image is yellowish.
An NCBI filter is not being used.
The lamp voltage is too low.
The image is too bright.
The lamp voltage is too high.
Inadequate illumination
(also check the electrical
system problems and
countermeasures)
The lamp voltage is too low.
Open the diaphragm to a suitable size.
(p.19)
The condenser is too low
Position the condenser so that the image
of the field diaphragm forms properly on
the specimen.
(p.18)
Focusing is not possible
with high-power objectives.
The specimen is placed upside-down.
we with the
Set the specimen on the
cover glass facing up.
The cover glass is too thick.
Use the specified type of cover glass
(thickness: 0.17 mm)
The objective strikes the
specimen when changing
from a low-power objective
to a high-power objective.
The specimen is placed upside-down,
'The cover glass is too thick
The diopter setting has not been adjusted.
Adjust the diopter setting.
(p.17)
The difference in focus is
large when the objective is
changed.
The diopter setting has not been adjusted.
Adjust the diopter setting
(p.17)
When viewing through
the binocular eyepiece,
the image does not resolve.
into a single image.
Adjust the interpupillary distance. (p.17)
The diopter setting has not been adjusted.
Eye strain develops while
viewing.
The interpupillary distance has not been
adjusted.
Adjust the interpupillary distance. (р.17)
The diopter setting has not been adjusted.
Adjust the diopter setting.
(p.17)
The brightness level is not suitable.
Adjust the brightness through the ND
filter combination.
(p.20)
36

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