Nikon ECLIPSE E400 POL Instructions Manual page 14

Switch to any desired objective 3
and view the specimen. / B Switch the objective.
Rotate the revolving nosepiece to
its click-stop position.
In the case of using an oil-
immersion objective, apply oil Ё
while being careful that air bubbles
do not form between the end of the dd
objective and the specimen.
Manipulate the coarse focus knob
or fine focus knob to re-focus on
the specimen.
E Readjust the focus.
~
Close the field diaphragm so that it
is just outside the field of view.
Ell Adjust the condenser
(p.20) aperture diaphragm.
| Close the condenser aperture
diaphragm to about 70 — 80% of
| the numerical aperture of the
* objective. (p.19)
EJ Adjust the field diaphragm.
This completes the procedure for microscopy for normal observation. Refer to the following for
details regarding the microscopy procedures for "Orthoscopic Observation" and "Conoscopic
Observation".
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