Nikon ECLIPSE E400 POL Instructions Manual page 27

Polarizing microscope
Hide thumbs Also See for ECLIPSE E400 POL:
Table of Contents

Advertisement

3. Operation of Each Part
m:
The P-CS Sénarmont compensator is used by inserting
into the slot of the intermediate tube in place of the
P-CL 1/47. & tint plate
It can be used to measure retardation up to 12. according
to the following procedure.
1 Determination of Extinction Position
Rotate the stage with the specimen under the crossed Nicols to find the direction where the
part of the specimen to be measured appears darkest
2 Determination of Subtraction Position
Rotate the stage 45? from the extinction position to the diagonal position.
Insert the P-CL.
1/47. & tint plate into the optical path and confirm that the interference color of the section
of the specimen to be measured changes toward the lower order side. If the interference
color changes toward the higher order side, rotate the stage another 90°.
3 Measurement
Place the G I F
filter on the field lens and replace the P-CL 1/47. & tint plate with the P-CS
Sénarmont compensator.
Rotate the analyzer so that the section of the specimen to be
measured becomes darkest. When the rotation angle of the analyzer at that time is taken to
(4: wavelength used)
The value of à when using the GIF filter is 546 nm.
25

Advertisement

Table of Contents
loading

Table of Contents