Nikon ECLIPSE E400 POL Instructions Manual page 23

3. Operation of Each Part
a Auto-photo switch (for photomicrography)
The color temperature of the lamp varies according to the voltage. If the voltage is high, the color
temperature of the lamp increases and the light becomes bluer. If the voltage is low, the color
temperature of the lamp decreases and the light becomes redder. Therefore, to obtain the best
color reproduction in color photomicrography, it is necessary for the lamp voltage to be kept
constant. When using daylight-type color film, the usual setup is to use the color balancing filter
(NCB11) and set to the standard lamp voltage.
'The auto-photo switch is used to automatically set the standard lamp voltage. If the images on
color film shot with the auto-photo switch on are reddish or bluish, finely adjust the voltage with
the auto-photo voltage selection switch. The standard
position for the auto-photo voltage selection switch with
four levels i s the second index dot from the innermost.
Sliding the switch forward increases the bluish tint of the
light, while sliding the switch towards the back increases
the reddish tint of the light.
Use commercially available color compensation filters
(CC filters) if this adjustment does not resolve the
problem.
These objectives are used with the immersion oil applied
between the specimen and the tip of the objective. The
immersion oil is provided for the microscope.
Bubbles in the oil will adversely affect the viewing of
the image, so be careful to prevent the formation of air
bubbles. To check for air bubbles, remove the
eyepieces, fully open the field and aperture diaphragms,
and look at the exit pupil of the objective within the
eyepiece tube. (The exit pupil will appear as a bright circle.) When it is difficult to see if there are
any bubbles, mount a centering telescope (sold separately) on the eye; sleeve with an adapter
(sold separately). Then, while turning the eyepiece on the centering telescope to change the focus.
look through the centering telescope for air bubbles. If there are bubbles in the oil, remove them
by one of the following methods:
" Turn the revolving nosepiece slightly, moving the oil-immersed objective back and forth once
or twice.
* Add more oil.
* Remove the oil and replace it with new oil.
Use as little oil as possible (just enough to fill the space between the tip of the objective and the
specimen). If too much oil is applied, the excess will flow onto the stage.
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