Cleaning Procedures; Ceramic Strip Holders/Manifold - GE Ettan IPGphor 3 Instrument User Manual

4
Care and maintenance
50
4.2

Cleaning procedures

IPGphor Strip Holder Cleaning Solution has been specifically formulated for
removing protein deposits. It will not damage the strip holder or manifold.
IPGphor Strip Holder Cleaning Solution (80-6452-78) can be ordered in 950-ml
bottles from GE Healthcare.
4.2.1

Ceramic strip holders/manifold

Ettan IPGphor 3 strip holders and manifold are made from a thermally
The
conductive aluminum oxide ceramic. They have been surface-treated to
minimize adsorption and interaction with proteins. The surface coating is
mechanically robust and can withstand temperatures of up to 300 ºC, however it
can be damaged by prolonged exposure to extremes of pH.
Clean strip holders/manifold after each first-dimension IEF run. Do not let
solutions dry in the strip holder/manifold. Cleaning may be more effective if the
strip holder/manifold is first soaked a few hours or overnight in a solution of 2–5%
IPGphor Strip Holder Cleaning Solution in water. Strip holders and manifold may
also be immersed in boiling 1% SDS.
IMPORTANT! You must use a neutral pH detergent, such as the IPGphor Strip
Holder Cleaning Solution, to remove residual protein from the strip holders/
manifold.
IMPORTANT! Fixed length strip holders and manifold may be baked, boiled or
autoclaved. DO NOT EXPOSE THEM TO STRONG ACIDS OR BASES, INCLUDING
ALKALINE DETERGENTS.
Cleaning procedure
1 First rinse off the strip holder/manifold. Use a mild liquid soap to remove any
residual IPG cover fluid.
2 Squeeze a few drops of IPGphor Strip Holder Cleaning Solution into the strip
holder/manifold slot. Use a toothbrush and vigorous agitation to clean the
strip holder/manifold.
3 Rinse well with distilled or deionized water.
4 Thoroughly air dry the strip holders/manifold or dry well with a lint-free
tissue prior to use.
5 Wipe the platform and plate electrodes with a lab tissue or paper towel after
each use. Other exterior surfaces can be cleaned with a damp cloth. Never
use abrasive cleanser or solvents.
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB