GE Ettan IPGphor 3 Instrument User Manual page 26

3
Operating instructions
26
Rehydrate the Immobiline DryStrip
1 Prepare the rehydration solution and rehydrate the Immobiline DryStrip in
the strip holder or in the rehydration tray when using the manifold.
2 Follow the recommended procedures found in the Instructions attached to
each Immobiline DryStrip package.
Note: See also the 2-D Electrophoresis, Principles and Methods guide containing
commonly used recipes.
Pipet the appropriate volume of rehydration solution into each holder.
CAUTION! Always wear protective gloves when working with rehydration
solution.
IMPORTANT! To ensure complete sample uptake, do not apply excess
rehydration solution.
The correct volume to each strip length is found in the instructions attached to
each Immobiline DryStrip package.
1 Deliver the solution slowly at a central
point in the strip holder channel away
from the sample application wells.
2 Remove any larger air bubbles.
Position the IPG strip
1 Remove the protective cover from the IPG
strip.
2 Position it with the gel side down and the
pointed (anodic) end of the strip directed
toward the pointed end of the strip holder.
3 Pointed end first, lower the strip onto the
solution. To help coat the entire strip,
gently lift and lower the strip and slide it
back and forth along the surface of the
solution, tilting the strip holder slightly as
needed to assure complete and even
wetting.
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB