GE Ettan IPGphor 3 Instrument User Manual page 42

3
Operating instructions
42
The original factory programmed time for each step is 0:00, and should be set to
0:00 for all unused steps. The Vhrs value is calculated according to the
explanatory note at the left, but these values can be set in the same manner as
the Hrs values.
Note: When toggling from Hrs to Vhr, the value in the Vhrs field is automatically
calculated according to the selected voltage change pattern:
For Step-n-hold, Vhr is the product of hours and voltage.
For Gradient, Vhr is the product of hours and the mean of the voltage limit
set for the current step and the previous step.
Absolute Vhr limits can be set to any value from 1–30000 Vhr.
P r o t # 1 3 S t e p s
S 1 G r a d 5 0 0 V 1 : 0 0 H r s
S 2 G r a d 1 0 0 0 V 4 : 0 0 H r s
S 3 S t e p 8 0 0 0 V 8 0 0 0 0 V h r
P r o t # 1 3 S t e p s
S 1 G r a d 5 0 0 V 1 : 0 0 H r s
S 2 G r a d 2 0 0 0 V 1 : 0 0 H r s
S 3 S t e p 8 0 0 0 V 2 : 0 0 H r s
Programming example
Simplified 4-step protocol and resulting voltage profile
To illustrate the two voltage change patterns, a simplified example shows
program steps and the resulting voltage profile. Note that step 4 ends the
protocol.
For recommended IEF protocols, see the 2-D Electrophoresis Principles and
Methods guide.
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
hours step
volt-hours step