Optional: Applying Electrode Pads Prior To Ief; Optional: Applying Sample After Rehydration - GE Ettan IPGphor 3 Instrument User Manual

3
Operating instructions
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3.3.3

Optional: Applying electrode pads prior to IEF

Under certain conditions, such as prolonged focusing, water may migrate toward
one end of the strip, causing the other end to begin drying out. This effect can be
minimized by placing paper electrode pads between the IPG strip and each strip
holder electrode just before IEF. Electrode pads may also absorb ions that would
otherwise accumulate at the ends of the IPG strip and possibly interfere with the
separation.
Applying electrode pads
1 Cut two 3 mm wide electrode pads from a paper IEF electrode strip
(18-1004-40).
2 Position on a clean, flat surface such as a glass plate and soak with deionized
water. Remove excess water by blotting with tissue paper.
IMPORTANT! Electrode pads must be damp, not saturated or dripping.
3 Using forceps or tweezers, lift one end of the rehydrated IPG strip. Position an
electrode pad over the electrode, then lower the strip back in place.
4 Repeat at the other end.
3.3.4

Optional: Applying sample after rehydration

If the sample was not applied by inclusion in
the rehydration solution, it can be applied
immediately prior to IEF.
Note: The IPG strip backing is impermeable;
do not apply the sample to the back
of the strip.
1 Prepare the sample in a solution similar
in composition to the rehydration
solution used.
2 Pipet the sample into either or both of
the lateral wells at either end of the strip
holder. Introduce the sample below the
cover fluid.
Up to 7.5 µl of sample solution can be added to each side (i.e. 15 µl per well or 30 µl
max. if both sides of both wells are used).
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB