GE Ettan IPGphor 3 Instrument User Manual page 56

5
Troubleshooting
Symptom
Sparks or burning in strips
56
Possible solution
Reduce the current limit. Do not exceed 50 µA per strip.
Prevent the IPG strip from drying out by always applying Immobiline
DryStrip Cover Fluid during rehydration.
Ensure that the IPG strip is fully rehydrated along the entire length of
the strip. The IPG strip should be in complete contact with the correct
volume of rehydration solution. Remove any air bubbles trapped under
the IPG strip.
Desalt the sample or prepare the sample so that the salt concentration
is less than 10 mM. De-ionize additives to the rehydration solution.
Excessive charged material in the sample or rehydration buffer lead to
electro-endosmosis which will dry out the middle of the strip, possibly
leading to arcing and burning at these points.
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB