Page 1 Operating Manual ® HAPSITE 074-471-P1E...
Page 2 INFICON Two Technology Place East Syracuse, NY 13057-9714...
Page 3: Table Of Contents
5 Operating Manual Style Conventions...................... 18 6 Customer Support............................ 19 6.1 How to Contact Customer Support ...................... 19 6.2 Returning Your Instrument to INFICON .................... 19 7 Introduction .............................. 21 7.1 System Description .......................... 21 7.2 Instrument and Accessories........................ 21 7.3 Specifications ............................
Page 4 8.3.6 Removing the Battery ......................... 48 8.4 Helpful Guidelines .......................... 50 9 Operate the Instrument in Portable Mode.................... 51 9.1 Start HAPSITE ER in Portable Mode ..................... 51 9.1.1 Emergency Mode (EMER MODE) .................... 59 9.1.2 Concentrator Options (CONC OPTIONS)................... 63 9.1.2.1...
Page 5 INFICON Table of Contents 10.1.2 Turning On the Radio........................ 110 10.1.3 Wireless Module Indicator Lights .................... 112 10.2 Setting Up Communications......................... 112 10.3 Configuring the Instrument for Communications.................. 119 10.3.1 Turning Off the Radio........................ 130 10.4 Wireless Information .......................... 132 10.4.1 Regulatory Compliance Information for UNITED STATES Users..........
Page 6 Table of Contents INFICON 12.3.3.1 Port Setting Tab....................... 158 12.3.3.2 Display Tab........................ 159 12.3.3.3 Miscellaneous Tab...................... 160 12.3.4 Tools Menu .......................... 167 12.3.4.1 Set Access Level ...................... 168 12.3.5 View Menu .......................... 170 12.3.6 Window Menu ........................... 171 12.3.7 Help Menu.......................... 171 12.4 Safety Database...........................
Page 7 INFICON Table of Contents 13.6.1 Setting the AMDIS Pathway...................... 224 13.6.2 View Function in AMDIS ...................... 227 13.6.3 Confirm Screen in AMDIS...................... 228 13.7 NIST Library Searches......................... 230 13.8 Current Peaks ............................ 235 13.8.1 Show/Update Current Peaks .................... 236 13.8.1.1 Show/Update Current Peaks Window Description ............ 241 13.8.1.2 Peak Search Parameters....................
Page 8 Table of Contents INFICON 14.4.1 Manual Tune Variables ...................... 283 14.4.2 *Outputs ............................ 284 14.4.3 *Inputs............................ 285 14.4.4 Other Inputs .......................... 287 14.4.5 *Set Access Level to Advanced .................... 288 14.4.6 *Manual Tune.......................... 288 14.4.7 *Save Tune .......................... 289 14.4.8 Tool Bar ............................ 289 14.4.9 Tune Drop-Down Menu......................
Page 9 INFICON Table of Contents 15.6.5 SituProbe Flow Parameter...................... 321 15.6.6 Scan Events for SIM Methods .................... 322 15.7 Tune Page............................ 322 15.7.1 Param Page .......................... 322 15.7.2 Report Page.......................... 324 15.8 Full Scan Page............................. 325 15.9 SIM Page ............................. 327 15.9.1 SIM for Analyze.........................
Page 10 Table of Contents INFICON 16 Calibration.............................. 370 16.1 Introduction to Quantitative Analysis.................... 370 16.2 Calibrating a Method .......................... 370 16.3 Definition of Terms in the Calibrate Window .................. 390 16.3.1 Method ............................ 391 16.3.2 Data Files.......................... 391 16.3.3 Peak Search .......................... 391 16.3.4 Analytes ............................
Page 11 INFICON Table of Contents 18.3 HAPSITE Spare Parts.......................... 418 18.4 HAPSITE Consumables........................ 419 18.5 Service Module Spare Parts ........................ 419 19 Glossary .............................. 420 20 Appendix A: HAPSITE Target Compounds.................... 424 20.1 Compounds in Order of Elution...................... 424 21 Appendix B: Calibrating Gas Mixtures..................... 426 21.1 Acquisition, Preparation, and Handling ....................
Page 12: Declaration Of Conformity
1 | Declaration of Conformity INFICON 1 Declaration of Conformity 12 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 13 INFICON Declaration of Conformity | 1 074-471-P1E HAPSITE ER Operating Manual 13 / 436...
Page 14: Two Year Warranty
Seller product was not designed nor against any defects due to plans or instructions supplied to the Seller by or for Buyer. This manual is intended for private use by INFICON Inc. and its customers. Contact INFICON before reproducing its contents.
Page 15 Two Year Warranty | 2 These instructions do not provide for every contingency that may arise in connection with the installation, operation or maintenance of this equipment. Should you require further assistance, please contact INFICON. 074-471-P1E HAPSITE ER Operating Manual 15 / 436...
Page 16: Disclaimer And Copyright
The information contained in this manual is believed to be accurate and reliable. However, INFICON assumes no responsibility for its use and shall not be liable for any special, incidental, or consequential damages related to the use of this product.
Page 17: Definition Of Note, Hint, Danger, Warning And Caution Paragraphs
Failure to heed these messages could result in personal injury. WARNING Dangerous voltages are present, which could result in personal injury. WARNING High temperatures are present, which could result in personal injury. 074-471-P1E HAPSITE ER Operating Manual 17 / 436...
Page 18: Operating Manual Style Conventions
Graphical User Interface (GUI). Actions in the HAPSITE software GUI that are common to the Windows GUI are not explained in detail in this manual. Refer to the Windows documentation supplied by Microsoft®. 18 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 19: Customer Support
The online DOC form can be accessed at www.inficon.com/ service. All chemicals that have been analyzed by the HAPSITE ER should be reported on the DOC form in order for INFICON’s service personnel to take the proper safety precautions when performing the repair.
Page 20 Return Materials Authorization (RMA) Number, which signifies that INFICON has authorized the return. If a laptop is purchased through INFICON, the laptop warranty is the responsibility of the customer and is not supported by INFICON. Failure to follow these procedures will delay the repair of the instrument.
Page 21: Introduction
HAPSITE ER Chemical Identification System is designed to identify and quantify volatile organic compounds from the parts-per-million (PPM) to the parts-per-trillion (PPT) level. HAPSITE ER is a portable unit that collects and analyzes samples in the field using self-contained gas canisters and a chemically maintained vacuum source, while operating on battery power.
Page 22: Specifications
802.11G wireless or direct Ethernet 7.4 Instrument Overview A diagram of the major HAPSITE ER components is shown below, including the pumps used to provide flow and vacuum. Service Module components are also identified. Service and HAPSITE ER Modules contain a Vacuum Interconnect Valve and electrical connectors through which vacuum systems join.
Page 23: Description Of Subsystems
HAPSITE ER GC system utilizes nitrogen as the carrier gas to transport analytes through a column, of which the standard column is a narrow-bore fused silica tube 15 meters in length.
Page 24: Membrane Isolation Valve
7 | Introduction INFICON HAPSITE ER uses internal standards to verify the performance of the Gas Chromatograph and Mass Spectrometer. The internal standard is composed of two volatile organic gases which are injected into the sample inlet flow. The internal standards’...
Page 25 The result is a small burst of electrical current in response to each ion emerging from the mass selector. The signal from the electron multiplier is connected to the electronic amplifier and data-handling system outside the vacuum. 074-471-P1E HAPSITE ER Operating Manual 25 / 436...
Page 26: Vacuum System
The vacuum is initially created by the turbo-molecular and diaphragm pumps in the Service Module. When a good vacuum is achieved, the pumps in HAPSITE ER are turned on and the vacuum interconnect valve is closed. At this point, the Service Module can be disconnected.
Page 27: Electronic Systems
The laptop is linked to HAPSITE ER via a specific crossover cable or wireless connection. This linkage permits data and methods to be uploaded from HAPSITE ER. It also allows for new or modified methods to be downloaded to HAPSITE ER.
Page 28: Hapsite Components And Assemblies
8 | HAPSITE Components and Assemblies INFICON 8 HAPSITE Components and Assemblies 8.1 Ship Kit Packing Lists 8.1.1 930-850-G5, G6, G7, G8 Ship Kit Contents The following items are provided in a typical 930-850-G5, G6, G7, G8 HAPSITE ER Ship Kit. 036-0015 Shoulder strap 074-290...
Page 29 Power Supply Ship Kit Usage 930-469-P1 930-850-G5 110 V USA 930-469-P2 930-850-G6 230 V European 930-469-G3 930-850-G7 230 V UK 930-469-G4 930-850-G8 230 V Australia In two separate boxes, HAPSITE battery (930-4062-G1) 074-471-P1E HAPSITE ER Operating Manual 29 / 436...
Page 30: Basic Assembly
HAPSITE ER must be operated a minimum of every 3 weeks. Recommended storage is in Extended Standby. 8.2.1 Attaching the Probe The probe attaches on the top of HAPSITE ER. The probe has two connections: a LEMO® communication line and a Valco connector. 30 / 436...
Page 31 INFICON HAPSITE Components and Assemblies | 8 Remove the silver LEMO port cap from the HAPSITE ER by pulling it outwards. Store the port cap for future use. Unscrew the Valco connector port cap. Store the port cap for future use.
Page 32: Installing The Gas Canisters
8 | HAPSITE Components and Assemblies INFICON Insert the Valco connector into the top of the HAPSITE ER. Screw the Valco connector into place. Save all of the port Installing the Gas Canisters caps for further use. These caps are...
Page 33 CAUTION Do not open the front panel in a contaminated area. The carrier and internal standard gas canisters must be installed inside HAPSITE ER prior to sampling. Follow the instructions below to install the gas canisters into the spring-loaded slots inside the unit.
Page 34 INFICON Once inserted, press in the canister and PUSH lever together, then release the PUSH lever. Gently pull on the internal standard canister outwards. It should remain fastened inside the HAPSITE ER. 34 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 35 HAPSITE ER and/or canisters. Insert a purple banded carrier gas canister into the bottom round opening. This opening is marked with a purple stripe. Press the PUSH lever while inserting the carrier gas canister. 074-471-P1E HAPSITE ER Operating Manual 35 / 436...
Page 36 8 | HAPSITE Components and Assemblies INFICON Once inserted, release the PUSH lever. Gently pull the carrier gas canister outwards. It should remain fastened inside HAPSITE ER. 36 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 37: How To Remove A Gas Canister
HAPSITE ER. 8.2.3 How to Remove a Gas Canister Removing the gas canisters is advised when HAPSITE ER has been placed into Extended Standby. Also, the gas canister will need to be replaced when the canister is low.
Page 38 8 | HAPSITE Components and Assemblies INFICON ► Press the PUSH lever located to the right of the canister. ð The canister will release. 38 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 39 The internal standard canister will need to be replaces after 3 days of continuous use. These numbers are guidelines and will vary. WARNING Do not refill canisters. Bodily injury may result. Canisters are designed to be disposable and may fail if filling is attempted. 074-471-P1E HAPSITE ER Operating Manual 39 / 436...
Page 40: Connect The Power Supply
The connection with the gray cord is fitted with a LEMO connection. When facing the front panel, this cord will plug into the left side of HAPSITE ER. 40 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 41 Remove the silver plugs from HAPSITE ER . Store the plugs for future use. These plugs will be necessary when decontaminating the unit. Align the red dots on the LEMO connection with the red dots on HAPSITE ER. Insert the connection into the power port.
Page 42: Connecting The Laptop
• via the black crossover cable • via the wireless connection 8.2.5.1 Connect the Laptop with the Black Crossover Cable Unscrew the cap on the port which is located on the top, left side of HAPSITE 42 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 43 The crossover cable has two ends. One end has a screw top on the modular connector. This end connects to HAPSITE ER. Plug the modular connector with the screw top into the HAPSITE ER port. Screw the plug into place.
Page 44: Connect Laptop With Wireless Connection
INFICON Plug the opposite end into the laptop. ð Once connected, the crossover cable communication between HAPSITE ER and laptop computer is enabled. 8.2.5.2 Connect Laptop with Wireless Connection Refer to Communications and Touch Screen Options [} 110] for information on enabling the wireless connection.
Page 45: Battery
The battery can be charged using the battery charger. Alternately, it can be charged in the HAPSITE ER when it is connected to external power. However, the battery will charge more slowly when charging inside HAPSITE ER.
Page 46: Loading The Battery Charger
► Place the discharged battery into one of the charging receptacles. ð The respective indicator will turn green and charging will commence immediately. CAUTION Do not use excessive force when placing battery in the battery charger. 46 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 47: Understanding The Battery Charger Indicators
ð In the elongated triangle, green lighted numbers will be displayed. The highest illuminated number indicates the remaining percentage of battery charge, which is reported in 20% increments. If OVER is illuminated, the battery is fully charged. 074-471-P1E HAPSITE ER Operating Manual 47 / 436...
Page 48: Installing The Battery
8.3.6 Removing the Battery Firmly push in the battery until a faint click is heard. Push in the Release button, the black round button to the right of the battery. 48 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 49 Pull the battery out of its compartment while pressing the release button. CAUTION Do not expose the battery compartments to rain or other foreign material. Ensure that the area is dry and contaminant-free before opening the front panel. 074-471-P1E HAPSITE ER Operating Manual 49 / 436...
Page 50: Helpful Guidelines
• Place appropriate caps over openings before decontaminating. • Go into a potentially explosive environment without an LEL meter and safety checks. HAPSITE ER is • Use 5% to 10% bleach solution or local SOP to not intrinsically safe. decontaminate HAPSITE ER.
Page 51: Operate The Instrument In Portable Mode
Operate the Instrument in Portable Mode | 9 9 Operate the Instrument in Portable Mode 9.1 Start HAPSITE ER in Portable Mode Portable mode refers to using HAPSITE ER without the laptop computer. Required Materials: • HAPSITE ER (analytical module) •...
Page 52 9 | Operate the Instrument in Portable Mode INFICON Power on HAPSITE ER while connected to AC power. Using battery power to turn on and heat HAPSITE ER will consume over 40% of the battery's charge. HAPSITE ER boots in approximately one minute and senses which sample configuration (for example, concentrator) has been installed.
Page 53 Operate the Instrument in Portable Mode | 9 HAPSITE ER begins to prepare various components. These components include heating the HAPSITE ER and accessory heaters, running AutoTune, powering the NEG, and if necessary, running a concentrator cleanout. During the preparation period, the front panel displays the PREPARING SYSTEM message.
Page 54 If a component is going to be prepared, but the preparation process has not started, it is shown in yellow. If the system is not ready, the items that need to be prepared is shown in red. 54 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 55 Hold the probe in a clean environment for the duration of the cleanout. If the concentrator cleanout is not successful, see Concentrator Cleanout Failure [} 69]. When HAPSITE ER is ready to run samples, a green SYSTEM READY, SURVEY READY, or ANALYZE READY message displays. 074-471-P1E HAPSITE ER Operating Manual...
Page 56 9 | Operate the Instrument in Portable Mode INFICON 56 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 57 If the methods have different temperature setpoints, the method that has been prepared to run will have a green READY message next to the method name. If SURVEY READY is displayed, touch RUN SURVEY or push SURVEY RUN. 074-471-P1E HAPSITE ER Operating Manual 57 / 436...
Page 58 9 | Operate the Instrument in Portable Mode INFICON If ANALYZE READY is displayed, touch RUN ANALYZE or push ANALYZE RUN. 58 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 59: Emergency Mode (Emer Mode)
This is not recommended for everyday use. While Emergency Mode is active, the concentrator cleanout will continue to be skipped until Emergency Mode is exited. To place the system into Emergency Mode: Touch EMER MODE while the PREPARING SYSTEM message is displayed. 074-471-P1E HAPSITE ER Operating Manual 59 / 436...
Page 60 9 | Operate the Instrument in Portable Mode INFICON Alternately, use the arrow keys to highlight the EMER MODE button and push OK SEL. A confirmation message will be displayed. Touch Yes or push OK SEL to continue. 60 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 61 INFICON Operate the Instrument in Portable Mode | 9 The EMER MODE button will turn red when Emergency Mode is activated. 074-471-P1E HAPSITE ER Operating Manual 61 / 436...
Page 62 INFICON To exit Emergency Mode, touch the EMER MODE button. Alternately, use the arrow keys to highlight the EMER MODE button and push OK SEL. The EMER MODE button will turn gray. 62 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 63: Concentrator Options (Conc Options)
The CONC OPTIONS button has two selections: Concentrator Cleanout and Skip Conc Cleanout. When Concentrator Cleanout is selected, the HAPSITE ER will run a manual cleanout. When Skip Conc Cleanout is selected, HAPSITE ER will bypass the concentrator cleanout once while the HAPSITE ER is preparing.
Page 64 9 | Operate the Instrument in Portable Mode INFICON Touch Concentrator Cleanout or highlight Concentrator Cleanout using the arrow keys. Push OK SEL. 64 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 65 INFICON Operate the Instrument in Portable Mode | 9 ð The HAPSITE ER will run a concentrator cleanout. 074-471-P1E HAPSITE ER Operating Manual 65 / 436...
Page 66 9 | Operate the Instrument in Portable Mode INFICON When the cleanout is successful, the Concentrator Cleanout Succeeded message will be displayed along with the final TIC. Push OK to exit the screen. 66 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 67: Skip Cleanout
INFICON Operate the Instrument in Portable Mode | 9 9.1.2.2 Skip Cleanout Touch CONC OPTIONS or use the arrow keys to highlight the CONC OPTIONS button and push OK SEL. 074-471-P1E HAPSITE ER Operating Manual 67 / 436...
Page 68 9 | Operate the Instrument in Portable Mode INFICON Touch Skip Cleanout or highlight Skip Cleanout using the arrow keys. Push OK SEL. 68 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 69: Concentrator Cleanout Failure
Operate the Instrument in Portable Mode | 9 ð The system will not run a cleanout as part of its preparation. 9.1.3 Concentrator Cleanout Failure If the concentrator cleanout is successful, the screen will display the final TIC. 074-471-P1E HAPSITE ER Operating Manual 69 / 436...
Page 70 Touch Skip to start running a concentrator Analyze method. Touch Abort to return to the Main Screen. If Abort is touched, HAPSITE ER will show that the SYSTEM IS NOT READY. HAPSITE ER will re-run the cleanout as part of its preparation.
Page 71: Quick Reference Sop- Heat-Up And Tune
Press the POWER button on the front panel. HAPSITE ER will heat the necessary components and perform AutoTune. A prompt to run SURVEY or ANALYZE will appear when HAPSITE ER is ready to run a sample. If the default method is not the desired method, touch STOP PREPARE.
Page 72 9 | Operate the Instrument in Portable Mode INFICON The screen prompts, Are you sure you want to stop preparing this instrument? Touch Yes or using the arrow keys, highlight Yes and push OK SEL. 72 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 73 Scroll up or down using the scroll bar and touch the desired method to highlight it. When the desired method is highlighted, touch Select. Alternately, scroll up or down using the arrow keys. When the desired method is highlighted, push OK SEL. 074-471-P1E HAPSITE ER Operating Manual 73 / 436...
Page 74 Different Method Using the SELECT METHOD Icon [} 71] for further instructions on system preparation. If the SYSTEM READY, ANALYZE READY or SURVEY READY message is already displayed and the prepared method is not the desired one, touch SELECT METHOD. 74 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 75: Change The Default Method
OK SEL. HAPSITE ER begins preparing the new method. 9.2.1 Change the Default Method The default method for HAPSITE ER can be changed. By changing the default method, HAPSITE ER will prepare the newly selected method upon startup. Touch SELECT METHOD.
Page 76 9 | Operate the Instrument in Portable Mode INFICON Highlight the desired method. Touch the Set Default button. Upon the next startup, HAPSITE ER will begin preparing the new default method. 76 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 77: Show All
Operate the Instrument in Portable Mode | 9 9.2.2 Show All HAPSITE ER will only show methods that are compatible with the current sample and/ or accessory configuration. By checking the Show All box, all loaded HAPSITE ER methods will appear in the text box, regardless of configuration. Non-compatible methods will be shown in a lighter gray.
Page 78 If an Analyze method is going to be run after Survey, verify that the appropriate sample configuration (concentrator) is installed. When powered on or taken out of Extended Standby, HAPSITE ER automatically starts preparing an ER Survey and Analyze method if the probe is attached.
Page 79 INFICON Operate the Instrument in Portable Mode | 9 Using the touch screen, touch RUN SURVEY. Alternately, push SURVEY RUN using the push buttons. 074-471-P1E HAPSITE ER Operating Manual 79 / 436...
Page 80 The front panel momentarily displays a Scanning Starts In message before the Survey run starts. Sample air away from the point of the concern for one minute. Remember to note the background TIC. 80 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 81 Hold the probe over the sample of interest for up to one minute. A peak may appear if the compound present is greater than 1 ppm. A compound identification may also be present on the HAPSITE ER screen. 074-471-P1E HAPSITE ER Operating Manual...
Page 82 The CMPD ID screen can also be accessed by using the arrow keys to highlight CMPD ID and pushing OK SEL. To view the chromatogram while the method is running, touch GRAPH. Alternately, use the arrow keys to highlight GRAPH and push OK SEL. 82 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 83 When the TIC begins to increase, move the probe away from the sample of interest. Continue the run until the TIC level returns to the initial background TIC level that was noted in Step 8. 074-471-P1E HAPSITE ER Operating Manual 83 / 436...
Page 84 9 | Operate the Instrument in Portable Mode INFICON Monitor the side bar on the HAPSITE ER screen for guidance. The bar rises as the TIC increases and green signifies that the proper sampling distance is being maintained. To avoid saturation, remove the probe from the sample when the bar increases and turns yellow.
Page 85: Quick Reference Sop - Survey Method
(concentrator) is installed and the proper Analyze method is displayed on the screen. If powering on HAPSITE ER or exiting Extended Standby, HAPSITE ER automatically begins preparing Survey. If needed, touch PREPARE on the touch screen. Alternately, using the arrow keys, highlight PREPARE and push OK SEL.
Page 86: Analyze (Gc/Ms) Mode With The Concentrator
ER_Tri-Bed_PPB_Standard method. CAUTION The concentrator feature has increased sensitivity. Use the appropriate method to avoid saturating HAPSITE ER. 9.4.2 Tenax Concentrator This method is also used for analyzing samples with concentration levels in the low part per million to high part per trillion range.
Page 87 Operate the Instrument in Portable Mode | 9 The HAPSITE ER automatically starts preparing a concentrator method. If HAPSITE ER does not prepare the desired concentrator method, refer to Select a Different Method Using the SELECT METHOD Icon [} 71]. When the HAPSITE ER has finished preparing and the concentrator cleanout is successful, a SYSTEM READY message is displayed with a prompt to press Survey or Analyze to begin sampling.
Page 88 9 | Operate the Instrument in Portable Mode INFICON When the screen prompts Collect Sample Now, hold the probe over the sample. Continue to collect the sample during both the Line Purge and the ConcFill screens. 88 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 89 ð The current TIC ð The time left until the run finishes Touching a compound on the list will display Synonym and Exposure Limit information if it is contained in the NIOSH database. 074-471-P1E HAPSITE ER Operating Manual 89 / 436...
Page 90 To view the chromatogram while the method is running, touch GRAPH. Alternately, use the arrow keys to highlight GRAPH and push OK SEL. This screen displays: ð The TIC Max ð The current TIC ð The time left until the run finishes 90 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 91 Operate the Instrument in Portable Mode | 9 Touching the blue compound identification above the chromatogram will display Synonym and Exposure Limit information. A METHOD FINISHED message is displayed when the Analyze method has ended. 074-471-P1E HAPSITE ER Operating Manual 91 / 436...
Page 92: Quick Reference Sop - Concentrator Methods
When the run is complete, a Method Finished prompt will be displayed. 9.5 Detecting Hazardous Chemicals If the HAPSITE ER Analyze message turns red, the chemical's concentration is either approaching the IDLH limit or the chemical is a chemical warfare agent. In CMPD ID mode, the compound is highlighted in red and in GRAPH mode, the name of the compound is written in red.
Page 93: Help Icon
The Help icon is located on the front panel in the top right hand corner. Help can be accessed by touching the Help icon or pushing the HELP button that is located on the front panel. 074-471-P1E HAPSITE ER Operating Manual 93 / 436...
Page 94: Info Icon
The Book icon will give a more detailed summary of the function. 9.7 Info Icon The Info icon is located next to the Help icon in the right corner of the HAPSITE ER screen. Info can be accessed by touching the Info icon, or by pushing the STAT key until the NIOSH database is displayed.
Page 95 An important resource in this database is The NIOSH Pocket Guide to Chemical Hazards. To access the database, scroll to the fourth option on the list and touch The NIOSH Pocket Guide to Chemical Hazards link. 074-471-P1E HAPSITE ER Operating Manual 95 / 436...
Page 96 9 | Operate the Instrument in Portable Mode INFICON When the publication appears, touch INDEX with CHEMICAL NAMES and SYNONYMS. 96 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 97 INFICON Operate the Instrument in Portable Mode | 9 Scroll down to display an alphabet. Touch the first letter of the desired compound. 074-471-P1E HAPSITE ER Operating Manual 97 / 436...
Page 98 9 | Operate the Instrument in Portable Mode INFICON A list of the chemicals that start with the selected letter is displayed. 98 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 99 Scrolling down displays information about the exposure limit and the boiling point of the chemical. Boiling point is one of the factors that determines if the chemical can be detected by HAPSITE ER. See Specifications [} 22] for boiling point recommendations.
Page 100 9 | Operate the Instrument in Portable Mode INFICON To access information regarding IDLHs, touch the first hyperlink on the Info screen. Scroll down or touch DOWN until the IDLH-Chemical listing and Documentation link is displayed. Touch this link. 100 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 101 INFICON Operate the Instrument in Portable Mode | 9 Scroll down, press DOWN or use the down arrow to find the desired compound. Press the link to view the compound's information. 074-471-P1E HAPSITE ER Operating Manual 101 / 436...
Page 102 9 | Operate the Instrument in Portable Mode INFICON Information regarding the compound's NIOSH REL, OSHA PEL, and toxicity data is displayed. 102 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 103: Exit Menu
The EXIT menu is located on the top row of the front panel. This option will access Turn Off, Reboot, or Standby. Turn Off will shut down the HAPSITE ER power. Reboot will reset the microprocessor in HAPSITE ER and reload the drivers. It will also restart the operating system, HAPSITE ER program, and the front panel program.
Page 104 If using the push buttons, push OK SEL. A prompt is displayed to confirm the selection. For example, if Turn Off is selected, a prompt Do you want to shutdown the HAPSITE ER? Touch Yes or select Yes and push OK SEL to continue.
Page 105: Extended Standby
Extended Standby is the preferred storage mode. In this state, the NEG pump remains heated at 400°C and the ion pump continues pumping to maintain a vacuum in the mass spectrometer. HAPSITE ER turns off the heaters for all other components. When in Extended Standby, remove the gas canisters to avoid consumption.
Page 106 When the screen prompts, Do you want to go into Standby?, touch Yes. Alternately, use the arrow keys, highlight Yes and push OK SEL. Alternately, touch EXIT or use the arrow keys to highlight EXIT. Push OK SEL. 106 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 107 When the screen prompts, Do you want to go into Standby?, touch Yes. Alternately, use the arrow keys, highlight Yes and push OK SEL. HAPSITE ER goes into Extended Standby. Remove the gas canisters. 074-471-P1E HAPSITE ER Operating Manual 107 / 436...
Page 108: End Standby
9 | Operate the Instrument in Portable Mode INFICON 9.8.2 End Standby To end standby, touch END STANDBY or using the arrow keys, highlight END STANDBY and push OK SEL. 108 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 109 INFICON Operate the Instrument in Portable Mode | 9 When the system prompts, Do you want to end standby?, touch Yes. Alternately, highlight Yes using the arrow keys and push OK SEL. 074-471-P1E HAPSITE ER Operating Manual 109 / 436...
Page 110: Communications And Touch Screen Options
Refer to Connecting the Laptop [} 42] for instructions on attaching the laptop. 10.1.1 Wireless Range HAPSITE ER is equipped with an 802.11 b/g wireless adapter. The typical range for a single is 300 ft. (100 m) with no obstructions. The following may degrade the signal: • Metal buildings •...
Page 111 WLAN should illuminate. When the green lights are illuminated, the wireless radio is being powered. Replace the switch cover by placing it over the switch. Turn it clock-wise until finger tight. 074-471-P1E HAPSITE ER Operating Manual 111 / 436...
Page 112: Wireless Module Indicator Lights
(IEDs), or both, if HAPSITE ER is transmitting in the safety exclusion area around the device(s). Discussed below is a hardware switch to turn off the wireless radio so that HAPSITE ER may still be used in areas where these hazards are a concern. Consult applicable regulations and policies when using HAPSITE ER with the wireless device active in such environments.
Page 113 INFICON Communications and Touch Screen Options | 10 Alternately, push the STAT button until the HAPSITE ER icon is highlighted. Touch the HAPSITE System icon. 074-471-P1E HAPSITE ER Operating Manual 113 / 436...
Page 114 10 | Communications and Touch Screen Options INFICON Touch the HAPS button or use the arrow keys to highllight the HAPS button and push OK SEL. Locate and note the HAPSITE ER name. 114 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 115 INFICON Communications and Touch Screen Options | 10 Open ER IQ Software. From the System drop-down menu, select Properties. Click the HAPSITE List button. 074-471-P1E HAPSITE ER Operating Manual 115 / 436...
Page 116 10 | Communications and Touch Screen Options INFICON Enter the H Number into the Enter New HAPSITE Name or IP Address. Click Add. 116 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 117 INFICON Communications and Touch Screen Options | 10 The newly added HAPSITE ER will appear in the HAPSITE List. Press OK. Press OK on the System Properties window. 074-471-P1E HAPSITE ER Operating Manual 117 / 436...
Page 118 System Setup screen. If HAPSITE ER is displayed, as seen below, then communications have been established. If the HAPSITE ER icon is overlaid with a gray "X," HAPSITE ER is not trying to communicate with the computer. 118 / 436...
Page 119: Configuring The Instrument For Communications
INFICON Communications and Touch Screen Options | 10 If the HAPSITE ER icon is overlaid with a red "X," HAPSITE ER is not properly communicating with the laptop. For example, the cross-over cable is disconnected. If the HAPSITE ER icon is overlaid with a blue "X," communication has not been fully established.
Page 120 10 | Communications and Touch Screen Options INFICON Alternately, push the STAT button until the HAPSITE ER icon is highlighted. Touch the HAPSITE System icon. Touch the HAPS button or use the arrow keys to highlight the HAPS button and push OK SEL.
Page 121 Touch the NET button or use the arrow keys to highlight the NET button and push OK SEL. The IP Adress and the Netmask of HAPSITE ER is displayed. For example: 10.210.6.1.148/255.252.0.0. Each HAPSITE ER has a unique IP Address.
Page 122 On the laptop, click the Start button on Microsoft Windows. Mouse over the Settings option and click Network Connections. The following window is displayed. Double-click on the desired connection. Choose local area connection to troubleshoot a crossover cable. Choose Wireless Connection to connect wirelessly. 122 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 123 INFICON Communications and Touch Screen Options | 10 The Connection Status window opens. Click Properties. 074-471-P1E HAPSITE ER Operating Manual 123 / 436...
Page 124 Select Use the following IP address. Enter the first number of the IP address into the first slot. For example, if the IP Address is 10.210.6.148, enter 10 into the first slot. 124 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 125 Communications and Touch Screen Options | 10 For the second number of the IP Address, enter 210 if connecting with the cable and 209 if connecting with the wireless radio into the second slot. 074-471-P1E HAPSITE ER Operating Manual 125 / 436...
Page 126 For the third number of the IP Address, add 128 to the number in the IP address. In this example, adding 128 to 6 equals 134, so 134 is entered into the third slot. 126 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 127 Communications and Touch Screen Options | 10 The fourth number of the IP Address is entered into the fourth slot without modification. Therefore, in this example, 148 would be entered into the fourth slot. 074-471-P1E HAPSITE ER Operating Manual 127 / 436...
Page 128 10 | Communications and Touch Screen Options INFICON Enter in the Subnet mask exactly as displayed. 128 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 129 INFICON Communications and Touch Screen Options | 10 Click OK in the Internet Protocol Properties window to close it. 074-471-P1E HAPSITE ER Operating Manual 129 / 436...
Page 130: Turning Off The Radio
10 | Communications and Touch Screen Options INFICON Communication between HAPSITE ER and the laptop is now established as indicated by the absence of an "X" over the HAPSITE ER Sensor Icon in the System Setup screen. 10.3.1 Turning Off the Radio See the following procedure for instructions for turning off the radio when wireless communication is not desired.
Page 131 (IEDs), or both, if HAPSITE ER is transmitting in the safety exclusion area around the device(s). Discussed below is a hardware switch to turn off the wireless radio so that HAPSITE ER may still be used in areas where these hazards are a concern. Consult applicable regulations and policies when using HAPSITE ER with the wireless device active in such environments.
Page 132: Wireless Information
(IEDs), or both, if HAPSITE ER is transmitting in the safety exclusion area around the device(s). Discussed below is a hardware switch to turn off the wireless radio so that HAPSITE ER may still be used in areas where these hazards are a concern. Consult applicable regulations and policies when using HAPSITE ER with the wireless device active in such environments.
Page 133: Fcc Statement
To maintain compliance with FCC standards and regulations and to ensure the proper operation of the wireless communication system used within the HAPSITE ER instrument, ONLY use the antenna that was originally supplied with the instrument. If damage occurs to the original antenna, please contact the INFICON service department for a replacement antenna (see Customer Support [} 19] for contact...
Page 134: Regulatory Compliance Information For Canadian Users
10.4.2 Regulatory Compliance Information for CANADIAN Users This section of the Operating Manual lists Industry Canada (IC) compliance information for the HAPSITE ER system that contains the wireless communication option. This equipment contains an OEM Embedded Wireless Bridge (Ethernet to Wireless LAN) Module from Quatech Inc.
Page 135: European Usage Restrictions
European usage restrictions apply to this equipment. The end user must comply with the usage restrictions noted in the table below when operating this equipment in the countries that have restrictions. HAPSITE ER is marked with the following symbol: 074-471-P1E HAPSITE ER Operating Manual 135 / 436...
Page 136: European Emc Compliance Statement
Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, United Kingdom 10.4.3.2 European EMC Compliance Statement English Hereby, INFICON Inc. declares that this HAPSITE ER Portable GC/MS is in compliance with the essential requirements and other relevant provisions of Directive 1999/5/EC. Finnish INFICON Inc. vakuuttaa täten että HAPSITE ER Portable GC/MS tyyppinen laite on direktiivin 1999/5/EY oleellisten vaatimusten ja sitä...
Page 137 INFICON Communications and Touch Screen Options | 10 Dutch Bij deze verklaart INFICON Inc. dat deze HAPSITE ER Portable GC/MS voldoet aan de essentiële eisen en aan de overige relevante bepalingen van Richtlijn 1999/5/EC. French Par la présente INFICON Inc. déclare que l’appareil...
Page 138: European Safety Compliance Statement
For additional information concerning the directives and standards that this instrument complies with, please refer to the Declaration of Conformity [} 12] that is located in the front of this manual. 138 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 139: Laptop Operation
Press the POWER button on the front panel to turn on HAPSITE ER. HAPSITE ER takes 1-2 minutes to boot. If desired and equipped, HAPSITE ER can be used with the laptop computer via the wireless connection. Refer to Communications and Touch Screen Options [} 110] for additional information on set-up and usage.
Page 140: Sampling Procedure
11.2.1 Sampling Procedure Open the ER IQ software by double-clicking on the ER IQ icon. If the probe is attached, HAPSITE ER will begin preparing a Survey method. If this method is not the desired one, see Selecting a New Method [} 149].
Page 141 If the TIC does not increase, hold the probe over the sample of interest for up to a minute. If the TIC does not increase after a full minute, move the probe away from the sample. 074-471-P1E HAPSITE ER Operating Manual 141 / 436...
Page 142 ð Survey is a tentative identification. To confirm results, run an Analyze (GC/ MS) Method. Record the data file name to reference for later review. See Data Review [} 203] for instructions on recalling data. 142 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 143: Quick Reference Sop - Running Survey Mode
Survey does not show an increase in TIC (total ion count, which is a measure of response), use the ER_Tri-Bed_PPB_Standard method. CAUTION The concentrator feature has increased sensitivity. Use the appropriate method to avoid saturating HAPSITE ER. 074-471-P1E HAPSITE ER Operating Manual 143 / 436...
Page 144: Tenax Concentrator
Extended Standy (refer to Extended Standby [} 105]), HAPSITE ER will automatically start preparing a concentrator method. If the method that HAPSITE ER begins preparing is not the desired one, refer to Selecting a New Method [} 149]. Power on the laptop by pushing the POWER button. Open ER IQ Software by double-clicking on the ER IQ icon.
Page 145 Click the RUN button on the pop-up window or from the Control Panel on the screen. When the HAPSITE ER screen prompts Collect Sample Now, place the probe over the sample for the entire specified sampling time. Be sure to keep the probe over the sample for both the Line Purge and ConcFill events.
Page 146 11 | Laptop Operation INFICON CAUTION Do not place the sample probe in liquids while sampling. When prompted Sampling is Done on the HAPSITE ER screen, remove the probe from the sample source. 146 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 147 As the method runs, the chromatogram will begin to appear on the laptop screen. The message METHOD FINISHED will appear on the HAPSITE ER screen when the run is complete. An example of a completed chromatogram on the laptop is shown below.
Page 148: Quick Reference Sop - Tri-Bed Concentrator Method
HAPSITE ER or take the system out of Extended Standby. HAPSITE ER begins preparing a concentrator method. When HAPSITE ER has finished preparing, a prompt to press Run is displayed on the laptop screen. When the screen prompts Collect Sample Now For, hold the probe over the sample until the screen prompts Sampling is Done When the run is complete, a Method Finished prompt is displayed.
Page 149: Selecting A New Method
ð Use the buttons at the top of the dialog box to choose the methods on HAPSITE ER. Choose a folder that matches the sampling configuration of HAPSITE ER. The concentrator folder refers to the Probe accessory. In this example, the probe is installed.
Page 150 11 | Laptop Operation INFICON Click the desired method and then click OK. This example shows the ER_Air_Tri-Bed_PPB_Standard.mth method. 150 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 151 AutoTune (if required). A concentrator cleanout will also be run if needed. When it is finished heating, a prompt will appear to indicate HAPSITE ER is ready to run a sample. Click RUN. For detailed instructions, refer to ANALYZE (GC/MS) Mode with the Concentrator [} 86].
Page 152: Er Iq Software
12.1 HAPSITE Software - ER IQ ER IQ software is the laptop software that controls instrument operation, runs analyses, manages files and creates reports. Data collected with HAPSITE ER is viewed and interpreted using ER IQ. This software allows for use of the entire NIST mass spectral library.
Page 153: Introduction
Upon opening ER IQ, the first screen displayed is the System Setup Screen, which controls instrument operation. This screen is used to run analyses, access data files, create or edit methods, and set parameters of various HAPSITE ER components. 12.3.1 System Startup Menu The main menu toolbar includes File, Functions, System, Tools, View, Window, and Help options.
Page 154 • Print will print files and is active on the Data Review screen. • Print Preview will display an example of the final printing layout and is active on the Data Review screen. • Print Setup accesses the printer setup options. 154 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 155 • Exit closes ER IQ. 12.3.1.1.1 View Log HAPSITE ER will log errors, warnings, and events, if desired. See Parameters [} 196] for information on enabling this function. A warning signifies there is a problem with the unit, such as high pressure. If the warning is ignored, it will become an error. An example of an event is the system coming online or going offline.
Page 156 12 | ER IQ Software INFICON The log file will be displayed. 12.3.1.1.2 Log File Toolbar The following icons will be displayed on the Log File Toolbar. 156 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 157: Functions Menu
The Run Method, Calibrate, Overlay, and Front Panel Display options function identically to the icons of the same name. 12.3.3 System The System menu is shown below. Clicking Properties will open the System Properties window. 074-471-P1E HAPSITE ER Operating Manual 157 / 436...
Page 158: Port Setting Tab
Port Settings is the default tab in the System Properties window. HAPSITE ER is configured at the factory to connect to the laptop. However, the HAPSITE ER option allows the user to add a different HAPSITE ER to the laptop or connect a HAPSITE ER to a new laptop.
Page 159: Display Tab
ER IQ Software | 12 12.3.3.2 Display Tab The Display tab is used to change the appearance of the ER IQ settings, including the thickness of the chromatogram line, the fonts used, and the screen layout. 074-471-P1E HAPSITE ER Operating Manual 159 / 436...
Page 160: Miscellaneous Tab
The Miscellaneous tab displays the defaults pathways, the data file increment digits, the software, safety and library pathways, the scaling preferences for the chromatogram, and the option to select Wizard Mode for Method Editor. 160 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 161 Four default software pathways are displayed. The Browse buttons access folders to reset the pathways, if necessary. However, the software installation should properly select these options. If a pathway requires resetting, follow the instructions below. Click Browse for the Default HAPSITE Folder. 074-471-P1E HAPSITE ER Operating Manual 161 / 436...
Page 162 12 | ER IQ Software INFICON The Select Base Folder window will open. Click to highlight the desired folder. ð Click Refresh to update the displayed folders if the desired folder is not displayed. 162 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 163 For example, if 2 is selected, the file name would read Method_yearmonthday_01. If 3 is selected, the file name would read Method_yearmonthday_001. The data file increment digits can also be selected in Method Editor. See Data File Information [} 362] for instructions. 074-471-P1E HAPSITE ER Operating Manual 163 / 436...
Page 164 12 | ER IQ Software INFICON The Method Editor in Wizard Mode checkbox is the next option. 164 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 165 INFICON ER IQ Software | 12 The Wizard Mode will guide the user through the Method Editor software by using Next > and < Back buttons at the bottom of the screen. 074-471-P1E HAPSITE ER Operating Manual 165 / 436...
Page 166 12 | ER IQ Software INFICON If the Method Editor in Wizard Mode box is not checked, tabs must be clicked at the top of the Method Editor screen to access method writing options. 166 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 167: Tools Menu
System Properties functions identically to the System Properties option in the System menu. Refer to System [} 157] for further information. Sensor Properties functions identically to the Properties option in the System Menu. Refer to System [} 157] for further information. 074-471-P1E HAPSITE ER Operating Manual 167 / 436...
Page 168: Set Access Level
ER IQ will have restricted access. Click Yes if continuing is desired. To change the access level, click on the Tools menu on the System Setup Screen. Select Set Access Level... 168 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 169 ER IQ program, in the Status Bar. 12.3.4.1.2 Setting or Changing the Access Level Password To change the Advanced password, first enter advanced mode. Press the Change Password button. The window shown below will be displayed. 074-471-P1E HAPSITE ER Operating Manual 169 / 436...
Page 170: View Menu
The Sensor Toolbar is shown below. See HAPSITE Icons [} 201] for icon descriptions. Fig. 1: Sensor toolbar The Function Toolbar is only available when the Data Review screen is open. See Introduction [} 205] for icon descriptions. 170 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 171: Window Menu
Help Topics is not available at this time. Module Info shows the build version of various files and product number of the installed software. About ER IQ shows the installed software version. 074-471-P1E HAPSITE ER Operating Manual 171 / 436...
Page 172: Safety Database
The following screen is displayed. Click the NIOSH Pocket Guide to Chemical Hazards link or the link to the desired database. If clicking the NIOSH Pocket Guide to Chemical Hazards link, the following screen is displayed. 172 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 173 This screen displays the following options: Introduction, Index of Chemical Names, Synonyms and Trade Names, Index of Primary Chemical Names, Index of CAS Numbers, Index of RTECS Numbers and Appendices. Click the Index of Chemical Names, Synonyms, and Trade Names link. 074-471-P1E HAPSITE ER Operating Manual 173 / 436...
Page 174 INFICON This screen displays an alphabetized list compounds in the database. To search specific compounds by chemical name, synonyms, and trade names, click on the first letter of the compound of interest. 174 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 175: Manage File
12.5 Manage File The Manage Files function transfers files between HAPSITE ER and the laptop. Double-clicking this icon opens the window shown below. 074-471-P1E HAPSITE ER Operating Manual...
Page 176 Data files can only be transferred from the ER IQ to the laptop, they cannot be transferred from the laptop to HAPSITE ER. Method files can be transferred both from HAPSITE ER to the laptop and from the laptop to HAPSITE ER.
Page 177 The <-- Backup option will backup the desired files from HAPSITE ER onto the laptop. The <-- Backup All option will backup all of the files found on HAPSITE ER onto the laptop. The Backup options will copy the files with a .tgz extension, while the Copy option maintains the .hps or .mth file extensions.
Page 178 Type in the new name and click OK. The Delete option removes folders or files. To delete folders or files, highlight the desired folder or file and click Delete. 178 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 179 INFICON ER IQ Software | 12 After Delete is clicked, a confirmation window is displayed. Click Yes to delete the folder or file. To exit the screen, click Done. 074-471-P1E HAPSITE ER Operating Manual 179 / 436...
Page 180: Status Icon
After double-clicking the Status icon, the first window displayed is the Status window. This screen displays the current temperatures and pressures of the key components in HAPSITE ER. The battery status and internal standard canister status is also displayed. Additionally, the sample input device, for example, probe, is shown.
Page 181: Hapsite Time Zone
ER IQ Software | 12 12.6.2 HAPSITE Time Zone The HAPSITE ER Time Zone tab allows the user to set the time on the HAPSITE ER. Setting this parameter ensures that the data files are stamped with the proper date and time.
Page 182 12 | ER IQ Software INFICON Clicking Set HAPSITE Date/Time to PC Date/Time automatically synchronizes HAPSITE ER to the laptop date and time. 182 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 183 INFICON ER IQ Software | 12 Clicking the Set HAPSITE Date/Time button displays a date/time window. 074-471-P1E HAPSITE ER Operating Manual 183 / 436...
Page 184 12 | ER IQ Software INFICON 184 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 185 INFICON ER IQ Software | 12 Use the top arrow keys to select the correct time. 074-471-P1E HAPSITE ER Operating Manual 185 / 436...
Page 186 12 | ER IQ Software INFICON To select the proper date, use the arrow keys below the time to scroll to the current month. Click on the current date. 186 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 187 INFICON ER IQ Software | 12 The Synchronization option synchronizes the time on the HAPSITE ER to the PC, GPS, or both. If synchronization is not required, click None. 074-471-P1E HAPSITE ER Operating Manual 187 / 436...
Page 188 12 | ER IQ Software INFICON When all the parameters have been set, click OK. 188 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 189: Hapsite Information
12.6.3 HAPSITE Information The HAPSITE Information tab provides general information regarding the HAPSITE ER system. The top portion, Status Information, provides verification that the system is online. It will also notify the user when a method is running. The Version...
Page 190: Pressure Flows And Temperatures
The only pressure that can be changed by a user is the GC Column Pressure. When using HAPSITE ER, the BPFB internal standard should elute from the column between 3:40 and 3:50, with 3:45 being the optimal elution time.
Page 191: Neg Status
INFICON ER IQ Software | 12 12.6.5 NEG Status The NEG is a consumable item. NEG Status reports the number of hours that have been consumed. See NEG Troubleshooting [} 405]. 074-471-P1E HAPSITE ER Operating Manual 191 / 436...
Page 192 If Error is checked, an error message is displayed. If Warning is checked, a warning message is displayed, when a problem, such as high pressure, arises. If Beep is checked, HAPSITE ER beeps when an error or a warning occurs.
Page 193 INFICON ER IQ Software | 12 When an error, warning or event occurs, HAPSITE ER stores information about the occurrence and date it occurred. The pathway where this data is stored is displayed. The desired number of days for log storage can be set or the logs can be stored indefinitely.
Page 194 12 | ER IQ Software INFICON The Directories folder allows the file pathway for HAPSITE ER to be set. All data and information that has been created by HAPSITE ER is stored in the folder that has been selected in the Sensor pathway. All data files that have been created by HAPSITE ER are stored in the folder that was selected by the Data pathway.
Page 195: Functions
12.6.6 Functions The icons shown on the Functions tab perform the same functions as the icons displayed in the System Setup screen. To activate a function, highlight the icon and press OK. 074-471-P1E HAPSITE ER Operating Manual 195 / 436...
Page 196: Parameters
It will also display the recommended preventive maintenance guideline of 1500 hours. If it needs to be replaced, the Replace button will activate. See information on contacting customer support for service. 196 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 197: Service Module
The Service Module can be used as an alternate vacuum source or as a troubleshooting accessory. For more information on using the Service Module, see Maintenance [} 402] or refer to the Service Module Operating Manual. 074-471-P1E HAPSITE ER Operating Manual 197 / 436...
Page 198: Front Panel Display Icon
12.7 Front Panel Display Icon Double-clicking on the Front Panel Display icon reveals an emulation on the laptop of the HAPSITE ER front panel screen, which can be used to control the front panel. Double-click the Front Panel Display icon to open the emulation.
Page 199: Hapsite Sensor Icon
All of the buttons on the emulation operate identically to their front panel counterparts. To utilize the emulation, click on the desired button. 12.8 HAPSITE Sensor Icon Right-clicking on the HAPSITE ER Sensor Status icon displays the following menu. 074-471-P1E HAPSITE ER Operating Manual 199 / 436...
Page 200: Update Hapsite Software
12.8.1 Update HAPSITE Software Periodically, a software update for HAPSITE ER may be available. Clicking the Update HAPSITE Software option, allows the user to select the software update file. Once selected, the update will be loaded onto the analytical module and the analytical module will restart.
Page 201: Disconnect
INFICON ER IQ Software | 12 12.8.3 Disconnect The Disconnect option will manually disconnect the laptop from HAPSITE ER and will remain disconnected until Bring Online is selected. CAUTION The Laptop and HAPSITE ER should always have the most current version of the software installed.
Page 202 Accesses the Service Module when attached. Accesses HAPSITE ER properties. Accesses the HAPSITE ER tune program. Opens the HAPSITE ER front panel display on the laptop screen. Accesses Data File information. Returns the current screen to the System Setup screen.
Page 203: Data Review
Follow the file selection procedure that was used in Step 2. Both chromatograms are displayed in the chromatogram window. The color displayed in the check box correlates with the color of the chromatogram. 074-471-P1E HAPSITE ER Operating Manual 203 / 436...
Page 204 Peaks can be aligned by retention time for further comparison. Determine the time difference between the peaks being compared. The chromatogram is shifted the desired amount of time by selecting + or - and typing in the time difference. 204 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 205: Introduction
The Data Review toolbar is shown in the figure below. Aborts a running method. Start/stops a method. Pauses the data updates to the laptop during a run. This does not pause the analysis of the data by the HAPSITE ER. 074-471-P1E HAPSITE ER Operating Manual 205 / 436...
Page 206: Accessing The Data Review Feature
The Data Review feature can be accessed as follows: Double-click the Data Review icon. Alternately, right-click the HAPSITE Sensor icon. The menu shown in the figure below is displayed. Click Data Review. 206 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 207 The Recall window will be displayed. Select PC if the file was run using the laptop. Select HAPSITE if the file was run using the HAPSITE ER front panel and the laptop was not connected at the time of sample collection.
Page 208 13 | Data Review INFICON Double-click on the desired data file. 208 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 209 INFICON Data Review | 13 ð HAPSITE ER data file extensions end in .hps. The Data Review screen with the selected data file will be displayed. The Data Review screen is divided into four sections, as shown below. 074-471-P1E HAPSITE ER Operating Manual...
Page 210: Reports
The mass spectrum generated from the sample is displayed in this of sample window. (Spectrum) 13.5 Reports To access data reports, double-click the View Search Results icon on the Data Review screen. 210 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 211 The Quantitative report can be exported to Excel for further analysis by clicking on the Export to Excel button. The Summary report includes: • date • time • method name • method description 074-471-P1E HAPSITE ER Operating Manual 211 / 436...
Page 212 • scan number • retention time • number of hits (possible identifications) • area • percent area • analyte identification • formula • fit (see Analyzing Data Using AMDIS [} 222]) • CAS number 212 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 213 • scan number • internal standard • retention time • number of hits (possible identifications) • area • percent area • analyte identification • formula • fit (see Analyzing Data Using AMDIS [} 222]) 074-471-P1E HAPSITE ER Operating Manual 213 / 436...
Page 214 13 | Data Review INFICON • purity • CAS number • concentration with units • RFC • The flag, which provides the reason that the compound was not identified. 214 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 215: Using The Zoom Function In The Tic/Ric Window
In order to magnify the peaks, ER IQ has a zoom capability. Click the Zoom icon. Two vertical lines are displayed. Mouse over one of the lines. The cursor becomes a double-sided arrow. Move the line to the desired point. 074-471-P1E HAPSITE ER Operating Manual 215 / 436...
Page 216 Mouse over the other line. The cursor will again become a double-sided arrow. Move the line to the desired point. Move the cursor between the two vertical lines. The cursor will become a magnifying glass. 216 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 217 INFICON Data Review | 13 Click between the two lines to zoom. Alternately, a zoom can be completed by clicking and holding the left mouse button at the desired zoom starting point. 074-471-P1E HAPSITE ER Operating Manual 217 / 436...
Page 218 Continue to hold the left mouse and drag the mouse to the desired zoom ending point. Two vertical lines are displayed. Release the left mouse button. Move the cursor in between the two vertical lines. The cursor becomes a magnifying glass. 218 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 219: Zooming Out
INFICON Data Review | 13 Click between the lines to zoom. 13.5.2 Zooming Out Click on the Zoom Out icon. 074-471-P1E HAPSITE ER Operating Manual 219 / 436...
Page 220: Using The Zoom Spectrum Function
Alternately, click F11 to return to the expanded view. 13.5.3 Using the Zoom Spectrum Function The Spectrum window has a zoom function to magnify the spectra. Click the Zoom icon. A rectangle is displayed in the Spectrum window. 220 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 221 If necessary, repeat with the other sides of the rectangle in order to adjust the desired zoom area. Move the cursor into the center of the rectangle. The cursor becomes a magnifying glass. Click inside the rectangle to zoom. 074-471-P1E HAPSITE ER Operating Manual 221 / 436...
Page 222: Analyzing Data Using Amdis
AMDIS is an acronym for Automated Mass Spectrum Deconvolution and Identification System, a tool which was developed by the National Institute of Science and Technology. HAPSITE ER utilizes an on-board library, HAPSITE.msl, and the AMDIS deconvolution algorithm to make identifications. This library contains approximately 750 chemicals including chemical warfare agents and is able to identify complex mixtures, including co-eluting chemicals.
Page 223 Analyze Button Sets the library pathway, which may be necessary when reloading the ER IQ program. See Setting the AMDIS Pathway [} 224]. Help Detailed Help instructions about AMDIS. 074-471-P1E HAPSITE ER Operating Manual 223 / 436...
Page 224: Setting The Amdis Pathway
Function in AMDIS [} 227]. 13.6.1 Setting the AMDIS Pathway If AMDIS is reloaded onto the laptop, the library pathway may need to be reset. To reset the pathway: Double-click on Analyze..Double-click on Target Library. 224 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 225 Click Select New to change the library pathway. The HAPSITE.msl is located at the following pathway: C:\IQ Software\SearchLibraries. If custom libraries are used, they must be located in this directory. Highlight the library and click Open. 074-471-P1E HAPSITE ER Operating Manual 225 / 436...
Page 226 13 | Data Review INFICON Click Save. Click Run. 226 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 227: View Function In Amdis
3 of the previous section and selecting View. This function displays the components of the HAPSITE.msl library. This list can be sorted by retention index, name or CAS number. 074-471-P1E HAPSITE ER Operating Manual 227 / 436...
Page 228: Confirm Screen In Amdis
The Confirm page will be displayed. An arrow located about the peak indicates that a compound has been found. A T over the arrow indicates that the compound has been identified by AMDIS. 228 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 229 INFICON Data Review | 13 If a peak has not been identified, click on the arrow above it. The arrow will turn red. Click Analyze then click Go to NIST MS Program. 074-471-P1E HAPSITE ER Operating Manual 229 / 436...
Page 230: Nist Library Searches
SI number based upon purity and fit, the ratio of the intensities of the unknown spectra to the library spectra. ü Refer to Accessing the Data Review Feature [} 206] to open a data file. 230 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 231 A number of 700 and above indicates a good match. A number of 800 and above indicates a very good match. A number of 900 and above is an excellent match. A match of 1000 is a perfect match. 074-471-P1E HAPSITE ER Operating Manual 231 / 436...
Page 232 Therefore, if a compound has four duplicates and a high SI number, the confidence in the identification will be high. To view the spectra for a different library identification, highlight the name of the desired compound. 232 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 233 If the Description box is checked, the highlighted hit, the SI, the formula, and CAS number is displayed. If the Search Result Masses box is checked, the masses and relative intensities for the current scan is displayed as a table for the NIST library spectrum. 074-471-P1E HAPSITE ER Operating Manual 233 / 436...
Page 234 13 | Data Review INFICON If the Scan Spectrum Masses box is checked, the masses and relative intensities are displayed as a table for the unknown spectrum. 234 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 235: Current Peaks
The Show/Update Current Peaks function searches in the same manner as the Search NIST/User function. After performing the search, the software will list all of the compounds that were identified in the chromatogram. 074-471-P1E HAPSITE ER Operating Manual 235 / 436...
Page 236: Show/Update Current Peaks
13.8.1 Show/Update Current Peaks The Show/Update Current Peaks function is accessed by right-clicking on the chromatogram. Mouse over Peaks and click on Show/Update Current Peaks. ð The Show/Update Current Peaks window displays. 236 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 237 INFICON Data Review | 13 Check the Search in NIST box. 074-471-P1E HAPSITE ER Operating Manual 237 / 436...
Page 238 13 | Data Review INFICON Click Search. 238 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 239 INFICON Data Review | 13 A NIST search is performed on the peaks that NIST has located. The Hits Found column is populated. 074-471-P1E HAPSITE ER Operating Manual 239 / 436...
Page 240 13 | Data Review INFICON Each identification has a drop-down menu. Five hits are displayed in each menu. 240 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 241: Show/Update Current Peaks Window Description
In the chromatogram beneath the Show/Update Current Peaks window, each detected peak is labeled with a black triangle. Pink dots appear at the base of each peak. These dots are used to determine the peak area. 074-471-P1E HAPSITE ER Operating Manual 241 / 436...
Page 242 Show/Update Current Peaks window using the Range Tool. See Range Tool [} 256]. Hide Graph Checking the Hide Graph box will remove the graph from the Show/Update Current Peaks window. 242 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 243: Peak Search Parameters
If NIST is reloaded onto the laptop, the library pathway may need to be reset. If the libraries have properly loaded, the pathways will be set to folders displayed in the figure below. 074-471-P1E HAPSITE ER Operating Manual 243 / 436...
Page 244 13 | Data Review INFICON To reset the NIST Main Library: Click Browse..Select the NIST Main Library from the folder displayed. Click Select to set the library. 244 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 245 INFICON Data Review | 13 To reset the NIST Replicate Library: Click Browse..Select the NIST Replicate Library from the folder displayed. Click Select to set the library. 074-471-P1E HAPSITE ER Operating Manual 245 / 436...
Page 246 13 | Data Review INFICON To reset the NIST USER Library: Click Browse..Select the NIST USER Library from the folder displayed. Click Select to set the library. 246 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 247: Report Preview
INFICON Data Review | 13 13.8.1.4 Report Preview Report Preview reformats the Show/Update Current Peaks window into a text file. To view the report, click the Report Preview button. 074-471-P1E HAPSITE ER Operating Manual 247 / 436...
Page 248 13 | Data Review INFICON The report is displayed. 248 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 249 Data Review | 13 The report can be exported to Excel for further data analysis. Click Export to Excel. The report will need to be saved before Excel will open. Click Save. 074-471-P1E HAPSITE ER Operating Manual 249 / 436...
Page 250 13 | Data Review INFICON The report opens in Excel. 250 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 251: Background Subtract
Perform a NIST Library Search by checking the Search NIST/User box in the Control Panel. If the SI number is low and the background is high, select the blue Background Subtract triangle from the lower left side of the chromatogram. 074-471-P1E HAPSITE ER Operating Manual 251 / 436...
Page 252 13 | Data Review INFICON Drag to an area that is representative of the background on either side of the selected peak. The background masses at this location are automatically subtracted from the peak. 252 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 253 All background subtractions are indicated in the Spectrum window by the designation Scan Number - B1(range) - B2(range). To remove Background Subtract from the chromatogram, place the cursor over the Background Subtract triangle, right-click and select Remove. 074-471-P1E HAPSITE ER Operating Manual 253 / 436...
Page 254: Background Subtraction Using A Range Of Points
INFICON 13.8.2.1 Background Subtraction Using a Range of Points Background from a range of points can be subtracted, if desired. Place the Background Subtract triangle at the apex of the small peak. 254 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 255: Additional Features Of The Background Tool
This procedure can be repeated by using the second Background Subtract triangle. 13.8.2.2 Additional Features of the Background Tool By placing the mouse over a Background Subtract triangle and right-clicking, see the figure below, the following menu will be displayed: 074-471-P1E HAPSITE ER Operating Manual 255 / 436...
Page 256: Range Tool
Range Tool. Follow the instructions below to use the Range Tool. Place the cursor on the red-striped triangle, which is the Range Tool. Left-click, hold and drag the triangle to the location where the scans should be averaged. 256 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 257 The red range lines should intersect the peak sides at 50% of their height. The left and right side boundaries can be manually adjusted by clicking on the smaller red triangle and dragging it to the desired location. 074-471-P1E HAPSITE ER Operating Manual 257 / 436...
Page 258: Additional Features Of The Range Tool
Select Mass for Integration Selects the TIC or a specific mass fragment for reintegration. Show Integration Displays the integration on the x-axis. Show Retention Time Displays the retention time on the x-axis. 258 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 259: Displaying Reconstructed Ion Chromatograms (Ric)
Enter the name of the compound in the box on the top left of the screen. (i.e., benzene). In the bottom right box, the 10 Largest Peaks is listed. Make a note of the three largest mass peaks that are between 45-300 amu. 074-471-P1E HAPSITE ER Operating Manual 259 / 436...
Page 260 Enter the largest peak, 78 for benzene, into the Control Panel underneath the Mass column. Press Enter. The RIC plot is displayed underneath the TIC window. A new row will be created in the Control Panel for entering additional peaks. 260 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 261 INFICON Data Review | 13 Enter the two or more remaining peaks into the Control Panel. 074-471-P1E HAPSITE ER Operating Manual 261 / 436...
Page 262 The compound may be present in the unknown sample if all three peaks (mass fragments) align in the RIC plot. Use the Search NIST/User program to confirm identification of the suspected compound. 262 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 263: Chromatogram Subtract
This is generally used to subtract the blank from the sample and verify the presence of a compound of concern. Overlay the desired chromatograms by using Chromatogram Overlay. Refer to Chromatogram Overlay [} 203]. Select the peak for the desired compound. Record the TIC. 074-471-P1E HAPSITE ER Operating Manual 263 / 436...
Page 264 13 | Data Review INFICON Right-click on the chromatogram. Click Select Chro to Subtract. Select the desired file for subtraction from the drop-down menu. This will generally be the file for the blank. 264 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 265 INFICON Data Review | 13 Right-click on the chromatogram. Right-click on the chromatogram. Click Select Chro to Subtract. The desired chromatogram is subtracted. Note the new TIC of the selected peak. 074-471-P1E HAPSITE ER Operating Manual 265 / 436...
Page 266 13 | Data Review INFICON To return to the previous view, right click on the chromatogram and click Chro Subtract from the menu to deselect. 266 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 267: Right-Click Menus Within Data Review
Displays a text box to label the chromatogram. The location of Chromatogram the label can be adjusted with the cursor and saved with the data file. Scan Subtract Subtracts the current scan from the displayed RIC plots. 074-471-P1E HAPSITE ER Operating Manual 267 / 436...
Page 268 Previous Search View results from a previous search. (Drop- down menu of Results previously opened data files.) TIC Graph When checked, displays TIC window. RIC Graph When checked, displays the RIC window. 268 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 269: 1Properties Menu
Use Scan Range The masses for the scan range of the method will be displayed. Low Mass Limit HAPSITE ER will display masses in the spectrum above this limit. High Mass Limit HAPSITE ER will display masses in the spectrum below this limit.
Page 270: Spectrum Window
Unlock Mass Unlocks the mass cursors to reassign a new color to the mass Cursors fragment when using RIC plots. Spectrum Mass List Displays a report of all the masses in the spectrum. 270 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 271: Analyzing Data Using Nist
Clear NIST Grab file Clears the list of previously selected files. Switch to NIST Program (F8) Starts the NIST database program and will export any selected files to NIST database program. 074-471-P1E HAPSITE ER Operating Manual 271 / 436...
Page 272: Nist Database Program
The NIST database program is third-party software that is included with the HAPSITE ER. Instructions for using the software are located by selecting HELP from the Menu selection in either the AMDIS or NIST program. 272 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 273: Grab Spectra For Nist
Data Review | 13 13.11.5 Grab Spectra for NIST To export the spectrum to the NIST database: Double-click on the desired peak in the TIC/RIC window. Place the cursor in the Spectrum window and right-click. 074-471-P1E HAPSITE ER Operating Manual 273 / 436...
Page 274 13 | Data Review INFICON Select NIST. Click Grab Spectrum for NIST. Click Switch to NIST Program. 274 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 275 INFICON Data Review | 13 The identification is displayed on the screen. 074-471-P1E HAPSITE ER Operating Manual 275 / 436...
Page 276: Tune
AutoTune program, which automatically sets and adjust all parameters, however, the user can set the parameters by manual tuning. HAPSITE ER uses two gas internal standards which contain mass fragments that span the mass range of interest. The internal standards are: •...
Page 277: Viewing A Tune Report
Adjusting other parameters without proper training may damage the instrument. Allow HAPSITE ER to AutoTune. When AutoTune is finished, the message Final Results are on the LCD are displayed. Click OK. Close the manual tune screen by clicking the X on the top right corner.
Page 278 14 | Tune INFICON Select View Tune Reports from the drop-down menu. Highlight the default.tun file and press OK. 278 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 279 INFICON Tune | 14 The Tune Reports are displayed. Tune Reports are stored, by default, for 30 days. 074-471-P1E HAPSITE ER Operating Manual 279 / 436...
Page 280: Tune Report Options
Creates a text file of the tune report. Print Prints the selected tune report. To view the Tune Report from the front panel display, touch the HAPSITE ER icon or push the SYSTEM STAT button until the HAPSITE ER icon is highlighted.
Page 281 INFICON Tune | 14 Touch the TUNE icon. Touch the TUNE REPORT button or use the arrow keys to highlight the TUNE REPORT button. The last tune report is displayed. 074-471-P1E HAPSITE ER Operating Manual 281 / 436...
Page 282 14 | Tune INFICON To scroll through the tune report, use up or down arrows keys. Touch OK or push OK SEL to exit the screen. 282 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 283: Performing Manual Tune
INFICON Tune | 14 14.4 Performing Manual Tune Manual tunes are a standard routine maintenance practice for HAPSITE ER and should be performed once every 4 to 6 months to maintain optimal performance of the unit’s mass spectrometer. Routine manual tunes will improve the accuracy of compound identifications, and provide indications of the health of the mass spectrometer.
Page 284: Outputs
Base Peak Gain BPG influences sensitivity. (BPG) • HAPSITE ER ideal setting is 0.5 (range is 0.4 to 0.6) Ion Percentages Actual Percentages are listed in the Mass Calibration table, outlined in red. The range of acceptable values is presented in the 4 adjacent columns, outlined in blue in the figure below.
Page 285: Inputs
(height) of the mass. Ion energies should be adjusted after Target Resolutions. Ideal Values Displays the ideal values for Ion energies for each of the 10 mass fragments. Mass IE Low IE High 074-471-P1E HAPSITE ER Operating Manual 285 / 436...
Page 286 25 V. The normal operating range is 1000 to 1600 V, with newer units typically showing lower values and older units showing higher values. If your unit requires an EM volt of 1600 to 2000 to reach BPG of 0.5, please contact INFICON for support, as your unit may require service.
Page 287: Other Inputs
INFICON Tune | 14 Observing Base Peak Properly adjusted Base Peak Gain for a HAPSITE ER is between 0.4 to 0.6, ideally at Gain (BPG) 0.50. 14.4.4 Other Inputs If you are unable to attain a satisfactory tune using the Resolution, Ion Energies, and EM Volt, then further adjustments may be necessary.
Page 288: Set Access Level To Advanced
Click OK. It will take 10 to 20 seconds to initialize. During that time the Emission and EM boxes turn green. The NEG (non-evaporable getters) is consumed while Manual Tune is open. Adjust the tune parameters as discussed above until all values are within range. 288 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 289: Save Tune
Show Bounds Displays the peak centroid and the target peak width at 10% peak height. Save Tune Saves the tune file. Load Tune Loads a new tune file and restarts tuning. 074-471-P1E HAPSITE ER Operating Manual 289 / 436...
Page 290: Tune Drop-Down Menu
(Y-axis), based on Mass 117. Show Tune Status Displays the Tune and Mass Calibration Status panel. Panel Show Mass Displays the Mass Calibration Status panel. Calibration Status View Tune Reports Displays the Tune Reports screen. 290 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 291 AutoTune Sets the AutoTune Tolerance for mass resolution and mass Tolerances axis position. The Advanced tune functions should only be utilized under the direction of INFICON Support personnel. Show Tune Displays the EM Voltage, Ionizer control, Baseline, Parameters Threshold and Rod Polarity settings on the Control Panel.
Page 292: Tune Control Panel
14.4.10 Tune Control Panel The Tune Control Panel is located on the right side of the screen and displays the individual mass peak scans, the measured intensity and the resolution. 292 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 293: 1Tune Parameters
Range is 1000 to 2000. EM voltage Increases or decreases the gain of the system. EM voltage should be set to a value that achieves a Base Peak Gain between 0.4 and 0.6. 074-471-P1E HAPSITE ER Operating Manual 293 / 436...
Page 294: Peak Scan Window
Enables the cursor to select and drag a mass peak to a different position on the mass axis. 14.4.11 Peak Scan Window The Peak Scan Window, as shown below, can be used to manually tune the mass peak. 294 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 295: 1Peak Scan Window Control
Returns the window to the original X axis and Y axis scale. Zoom Out Y Axis Returns the Y axis to original scale. Y Axis Scale Increases or decreases the Y axis scale. 074-471-P1E HAPSITE ER Operating Manual 295 / 436...
Page 296: Setting The Full Scan Range
The scan ranges of 45 to 300 amu or 1 - 45 amu can also be selected. The EM voltage will automatically be decreased by 500 volts (default) whenever a range below mass 45 is scanned. 296 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 297: Tune And Mass Calibration Status
Displays the minimum target percentage required for the mass Percentage peak to meet OK criteria. If the actual percentage is below the minimum percentage, the box will turn red. Actual Percentage Displays the actual measured target percentage. 074-471-P1E HAPSITE ER Operating Manual 297 / 436...
Page 298: Mass Calibration Status
Outside of minimum value but within acceptable tolerance. OK HIGH Outside of maximum value but within acceptable tolerance. Below minimum value, needs adjustment. HIGH Above maximum value, needs adjustment. FAILED Cannot locate mass peak within window. 298 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 299: Scan Window Menu
Displays the Properties window. 14.4.16 Tune Status Window Menu Place the mouse cursor in the Tune Status panel or the Mass Calibration Status panel and right-click to display the menu shown in the figure below. 074-471-P1E HAPSITE ER Operating Manual 299 / 436...
Page 300 Tile Grids Vertically Tiles the Status and Calibration Status panels vertically. Size Columns to Resets the column size to the current grid. Grid Dock Locks the display position to a fixed position. Properties... Displays the Properties window. 300 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 301: Method Editor
Methods cannot be viewed, created or changed when the access level is set to Normal. Each page of the Method Editor shows a profile at the bottom of the Inlet States and Temperature. For questions relating to method development, please contact INFICON for application support. 074-471-P1E HAPSITE ER Operating Manual 301 / 436...
Page 302 To access Method Editor: On the System Setup screen, double-click on the Method Editor icon. If more than one unit is connected to the laptop, click on the name of the desired HAPSITE ER. 302 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 303: Reloading Default Hapsite Methods
INFICON Method Editor | 15 There are four options for accessing a method: Open Opens an existing HAPSITE ER method for modification. Method Open a blank method template to modify as necessary. Method Allows a method to be automatically Sequence repeated or a series of methods to be run together.
Page 304 Verify that ER is checked on the right side of the window. Highlight the desired method and click Load. See Default Methods [} 306] for a description of the methods. The Description page is displayed. 304 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 305 INFICON Method Editor | 15 Click Save at the bottom of the Method Editor-Description page. 074-471-P1E HAPSITE ER Operating Manual 305 / 436...
Page 306: Default Methods
Two digits are appended to the method file name (for example, 01). If the two digits are not desired, remove them before clicking Save. 15.3 Default Methods The methods found in the Default Methods window are general purpose methods for each of the HAPSITE ER configurations. ER_Air_Tri-Bed_PPB_Standard Carbon concentrator method (10 minute analysis time) ER_Air_Tri-Bed_PPM_Standard...
Page 307: Description Page
A temperature profile with the inlet states is displayed at the bottom of all of the method pages. 074-471-P1E HAPSITE ER Operating Manual 307 / 436...
Page 308 This mode scans all the masses across a given range, which is 42-300 for default methods. It is used to identify unknown samples. Full Scan is available for both Analyze (GC/MS) and Survey modes. 308 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 309 The figure below shows the Wizard Mode navigation buttons. In Non-Wizard mode, which is recommended only for experienced users, all pages are available through a tabbed window. To change the Wizard mode settings, refer to Miscellaneous Tab [} 160] for instructions. 074-471-P1E HAPSITE ER Operating Manual 309 / 436...
Page 310: Startup Page
15.5 Startup Page The Startup page, shown in the figure below, displays the initial settings for the HAPSITE ER system heaters. The initial temperature settings for the components described in HAPSITE Temperatures (C) can be modified on this page. The Sample Input Device (that is, the Probe, Headspace, SituProbe or SPME) can be selected on this page.
Page 311 SituProbe Select SituProbe when using this accessory to analyze volatile organic compounds. Internal Standard Box Use Internal The Use Internal Standard option is available when creating Standard Survey methods. 074-471-P1E HAPSITE ER Operating Manual 311 / 436...
Page 312 The target Transfer Line temperature setting for the SituProbe. Probe The target Probe temperature setting for the SituProbe sampling probe. SPME SPME Fiber Type the color of the fiber into the box. 312 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 313: Inlet Page
15.6.1 Inlet States Inlet States control the HAPSITE ER and accessory valve settings for sampling, analysis and purging of the HAPSITE ER. The figure below shows the grid used to program the Inlet States. 074-471-P1E HAPSITE ER Operating Manual...
Page 314 15 | Method Editor INFICON To edit the Inlet States grid, select an Inlet State from the drop-down menu. 314 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 315 Survey Turns on the sampling pump and directs the sample to the inlet of the MS. Other Customizes each specific GC valve for a custom GC valve state. Useful for GC troubleshooting. 074-471-P1E HAPSITE ER Operating Manual 315 / 436...
Page 316 HSSample Turns on the sample pump to direct sample through the transfer line to the HAPSITE ER. The suggested HSSample duration is approximately 15 seconds. HSPurge Directs carrier gas flow through the lines, the needle assembly and the transfer line to remove moisture and clean out the previous sample.
Page 317 INFICON Method Editor | 15 Upon entering the Duration settings, the Start time is automatically calculated for the next Inlet State. 074-471-P1E HAPSITE ER Operating Manual 317 / 436...
Page 318: Gc Temperature Profile
Rows cannot be inserted after the Standby event. 15.6.2 GC Temperature Profile GC Temperature Profiles specify the column temperature, ramp rate and hold settings for the HAPSITE ER Method. Adjusting the temperature program changes the retention times of the internal standards. 318 / 436...
Page 319: Scan Events
Hold Time adjusts the Start time of the next parameter. A maximum of four lines is permitted in this section. 15.6.3 Scan Events The items displayed in the Scan Events field is dependent upon the type of method. 074-471-P1E HAPSITE ER Operating Manual 319 / 436...
Page 320: Headspace Flow Parameter
CAUTION If the Filament delay is too short, the high pressure burst caused by a solvent peak may shut down HAPSITE ER and stop the analysis. The Run Time is the amount of time that the method will run. The type of concentrator being used is selected in the Concentrator box. The options are None, Tenax or Carbon.
Page 321: Situprobe Flow Parameter
The Scan Events field for the SituProbe has an additional parameter, SituProbe Flow Pressure. SituProbe Flow Pressure controls the flow rate of carrier gas through the SituProbe during the Sample and Purge states. This parameter is only available when creating SituProbe methods. 074-471-P1E HAPSITE ER Operating Manual 321 / 436...
Page 322: Scan Events For Sim Methods
This page also has a Show Details checkbox , which will produce a grid of tune parameters contained in the file. These parameters cannot be edited. If editing is desired, refer to Tune [} 276]. 322 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 323 INFICON Method Editor | 15 074-471-P1E HAPSITE ER Operating Manual 323 / 436...
Page 324: Report Page
15 | Method Editor INFICON 15.7.2 Report Page The Report page displays the AutoTune report in a printable format. 324 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 325: Full Scan Page
Scan Set Name for easy identification, if desired. The Filament Delay, from the Inlet Page (refer to Scan Events [} 319]), is also shown on the Full Scan page. Changing the Filament Delay on this page may require changes to the Inlet Page. 074-471-P1E HAPSITE ER Operating Manual 325 / 436...
Page 326 The longer the Dwell Time, the better the signal to noise ratio of the analyte. Run Time The time span of the method from start to finish. 326 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 327: Sim Page
Scan Sets grid. The Scan Sets fields, in the order recommended for editing, are as follows: Begin Time The start time for mass collection. End Time The stop time for mass collection. 074-471-P1E HAPSITE ER Operating Manual 327 / 436...
Page 328: Sim For Survey
To fill any column with the entry listed above, click in the desired cell and press Ctrl+D. 15.9.2 SIM for Survey The SIM page for Survey mode provides the ability to create only one scan set. 328 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 329: Creating A Sim Method
15.9.3 Creating a SIM Method ü Follow Step 1 through Step 4 of Reloading Default HAPSITE Methods [} 303]. Change the Collection Mode to SIM. 074-471-P1E HAPSITE ER Operating Manual 329 / 436...
Page 330 15 | Method Editor INFICON Select Next until the SIM page is displayed. 330 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 331 INFICON Method Editor | 15 For the Begin Time, enter in the number displayed in the Filament Delay. For default ER methods, this is 15 seconds. 074-471-P1E HAPSITE ER Operating Manual 331 / 436...
Page 332 15 | Method Editor INFICON Enter the desired End Time so that the Begin Time and the End Time surround the expected retention time of the compound. 332 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 333 INFICON Method Editor | 15 Enter the name of the chemical of interest. 074-471-P1E HAPSITE ER Operating Manual 333 / 436...
Page 334 15 | Method Editor INFICON Enter at least three mass fragments for the selected chemical of interest. 334 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 335 INFICON Method Editor | 15 It is recommended that the Dwell Time is adjusted until the Round Trip is approximately one second. 074-471-P1E HAPSITE ER Operating Manual 335 / 436...
Page 336: Search Page
15.10 Search Page The Search Page sets the necessary parameters to qualify and quantify data. To quantify data, a calibration library must be created. See Calibration [} 370], for instructions on creating a calibration library. 336 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 337 Quantitative Generates a Quantitative (Quant) report at the end of the run by referencing the designated library. Qualitative/ Searches AMDIS to provide identifications during a run and Quantitative generates a Quantitative report. 074-471-P1E HAPSITE ER Operating Manual 337 / 436...
Page 338: Setting Up A Qualitative Search
Qualitative. AMDIS will be used to identify the sample components. The search parameters can be modified using the Qualitative Search Settings button. The figure below lists the different analysis types available for the search. 338 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 339 RI Calibration/ This analysis establishes the correlation between the retention Performance time of a component and the retention index using the set of standards specified in the calibration library. 074-471-P1E HAPSITE ER Operating Manual 339 / 436...
Page 340 To view other library choices, select the Browse button. There are several small and recommends using specific libraries in addition to the HAPSITE.MSL. Many of the compounds found in HAPSITE.MSL these small libraries, that can be detected by the HAPSITE ER, are incorporated in the HAPSITE.MSL file. AMDIS Libraries: • HAPSITE.MSL •...
Page 341 Peak Shape The shape requirement allows all of the deconvoluted peaks to maintain the same shape. As the shape requirement increases, the shape of the individual ions will be more uniform. 074-471-P1E HAPSITE ER Operating Manual 341 / 436...
Page 342: Setting Up A Quantitative Search
Once a calibration library has been created, the Library Search Parameters button is activated. The Library Search Parameters functions set the peak identification criteria of the library compounds, as well as the unknown analytes. 342 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 343 INFICON Method Editor | 15 Clicking the Library Search Parameters button on the Method Editor Search page displays the following window. 074-471-P1E HAPSITE ER Operating Manual 343 / 436...
Page 344: Peak Search
The area of the intensity of the largest mass fragment of the peak must be above this setpoint. Min TIC The area of the intensity of the total ion count for the peak must be above this setpoint. 344 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 345 NIST will only use masses above this setpoint to make an identification. High Mass NIST will only use masses below this setpoint to make an identification. Minimum Match Factor The net fit in AMDIS must be above this number. 074-471-P1E HAPSITE ER Operating Manual 345 / 436...
Page 346: Alarm
On the main Method Editor Search page, the Alarm option in the Library Actions box activates when a calibrated library has been saved to the method. To enable the Alarm option: Check the Alarm box. 346 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 347 Enter the desired alarm level into the box with the units. The alarm is displayed when any analyte is detected at a concentration above the alarm level. To enter in alarm levels for individual analytes, see the figure below. 074-471-P1E HAPSITE ER Operating Manual 347 / 436...
Page 348: Edit Options
15 | Method Editor INFICON Click Yes to confirm that the alarm level is correct as entered. 15.10.5 Edit Options Clicking the Edit box displays the following information about the calibration library. 348 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 349 INFICON Method Editor | 15 The Analyte List is displayed. 074-471-P1E HAPSITE ER Operating Manual 349 / 436...
Page 350 Internal or Analyte is selected. If Internal is selected, the compound is an internal standard. If Analyte is selected, the compound is an analyte of interest. The Conc. (concentration) column is populated for internal standards. It is blank if the compound is an analyte. 350 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 351 Therefore, the analyst would enter 5 into the IS Ref column for trichloroethylene, because BPFB_117 is the closest internal standard. On the Mass Peaks tab, the mass fragments for the highlighted compound are displayed with their intensity. 074-471-P1E HAPSITE ER Operating Manual 351 / 436...
Page 352 15 | Method Editor INFICON On the Calibration tab, the calibration curve for the analyte is displayed. See Calibration [} 370]. For information on the Search Parameters tab, refer to Peak Search [} 344]. 352 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 353 INFICON Method Editor | 15 The Alarm tab allows an alarm level to be entered for each individual compound. To enter an alarm: Highlight the desired compound. Check the Alarm box. 074-471-P1E HAPSITE ER Operating Manual 353 / 436...
Page 354 Enter the desired concentration followed by the desired units. The Shift RTs option allows the predicted retention time for the highlighted analyte to be shifted by a desired amount of time. To shift the retention time: Click Shift RTs. 354 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 355 INFICON Method Editor | 15 The Shift RTs window is displayed. Select Add or Subtract from the menu. Type in the desired amount of time. 074-471-P1E HAPSITE ER Operating Manual 355 / 436...
Page 356: Template/Calibration Files
The first column will display the names of the files that were used to create the calibration curve, time that the file was saved, the concentration of the file and the units. 15.10.7 View Reports Clicking the View Reports button will display the Calibration Response Report. 356 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 357 Response Table. This table will display the file names, the area of the peak and the response factor (the ratio between the signal produced by the analyte and the quantity of the analyte which produces a signal). 074-471-P1E HAPSITE ER Operating Manual 357 / 436...
Page 358 By selecting analyte from the drop-down menu, the Analyte Standard Calibration Response Table will be displayed. The Analyte Standard Calibration Response Table contains the same information as the Internal Standard Calibration Table, but pertains to the calibrated analytes. 358 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 359 INFICON Method Editor | 15 Selecting All will display the information contained in the Internal Standard Calibration Report and the Analyte Standard Calibration Report. 074-471-P1E HAPSITE ER Operating Manual 359 / 436...
Page 360 The Cal/Quant Report will display the same information as a Quantitative Report. Refer to Reports [} 210] for more details. In the top right corner, the peak search parameters are also displayed. Refer to Peak Search [} 344]. 360 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 361: Data Page
INFICON Method Editor | 15 15.11 Data Page The Data Page customizes the names and the storage location of the data files for the method. 074-471-P1E HAPSITE ER Operating Manual 361 / 436...
Page 362: Data File Information
Use default When checked, the method will use a default format for the filename data filename. The default filename is a combination of the method filename, the date and the sample run number. 362 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 363: Date And Time Appendix
As Suffix: When Date and Time is selected, the date and time are added to the end of the filename. 074-471-P1E HAPSITE ER Operating Manual 363 / 436...
Page 364: Data Display
When Date and Time is selected, the date and time are added to the beginning of the filename. Save Data to Data is saved to the USB on the HAPSITE ER as well as to the HAPSITE ER hard Removable Drive: drive in the folder (directory) shown immediately above this check box.
Page 365: Summary Page
A series of methods can be configured to run back to back or at timed intervals. Follow the instructions below to sequence a method. Double-click the Method Editor icon. If more than one unit is connected to the laptop, click on the name of the desired HAPSITE ER. 074-471-P1E HAPSITE ER Operating Manual 365 / 436...
Page 366 Click the HAPSITE Method Sequence option. If desired, type in a new name for the method. Ensure that the file extension ends in .xmth. Click the button that is highlighted in the figure below. 366 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 367 The Immediately option runs the method as soon as the previous method has finished. The Run Button option requires the user to select Run to start the next method. Select the timing between sample runs. 074-471-P1E HAPSITE ER Operating Manual 367 / 436...
Page 368 Select Sleep until to enter a specific time. For example, if 1:30 is entered, the second method will start at 1:30 a.m. Sleep until uses 24 hour notation. Add multiple runs by repeating steps 5-10. Alternately, right-click and select Duplicate Row. 368 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 369 Select Save to save the method. Select the desired location for saving the method. The method can be saved to the laptop or the HAPSITE ER by clicking the desired option at the top of the window. 074-471-P1E HAPSITE ER Operating Manual...
Page 370: Calibration
16 Calibration 16.1 Introduction to Quantitative Analysis A HAPSITE ER method can be developed to collect and quantify sample data. Quantitative analysis involves creating a calibration library of target compounds, and associating target compound responses with concentration results. This library contains the analyte name, analyte area, the retention time and the response factor used to calculate the concentration of the analyte.
Page 371 INFICON Calibration | 16 ð Click OK. 074-471-P1E HAPSITE ER Operating Manual 371 / 436...
Page 372 Selecting the Calibrate function displays a dialog box used to select either an Analyze (GC/MS) method or a Survey method. The Select Method First box should remain checked. Click OK. The Method File window is displayed if the Select Method First box remained checked. 372 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 373 INFICON Calibration | 16 If the Select Method First box was unchecked, select Browse to select a method file. Click Browse under Data Files. Select the data file for library template creation. 074-471-P1E HAPSITE ER Operating Manual 373 / 436...
Page 374 If Global is selected, enter in the concentration of the standard. Step 12 is automatically completed if the information was entered in the Data File Information window when the sample was run. Refer to Steps 2–5. 374 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 375 Step 32. Set Peak Search to Search. Check Select. Click Start. All compounds that have been identified by AMDIS will be labeled with a red "T" over the apex of the peak. 074-471-P1E HAPSITE ER Operating Manual 375 / 436...
Page 376 The highlighted compound in the library template corresponds to the peak with the red dotted line. Verify that all analytes have a Net Fit greater than 70 by hovering the mouse over the analyte name. Verify that the retention times are correct. 376 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 377 If a compound was not correctly identified, type in the correct name. Alternately, the down arrow next to the compound name can be used to select a different name if AMDIS has identified more than one possible match. 074-471-P1E HAPSITE ER Operating Manual 377 / 436...
Page 378 Yes to allow the recalibration to continue. Enter the lowest concentration of each analyte to the Conc column if Analyte has been selected. If Global is selected, this step can be skipped. 378 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 379 Unwanted mass fragments would be those with intensities below 15%, unless the fragmentation pattern is not very distinct. Save the template by clicking Save Library. Enter a library name and a user name. 074-471-P1E HAPSITE ER Operating Manual 379 / 436...
Page 380 16 | Calibration INFICON Click OK. To calibrate the library, click Browse and select the desired data files. 380 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 381 INFICON Calibration | 16 074-471-P1E HAPSITE ER Operating Manual 381 / 436...
Page 382 16 | Calibration INFICON Select Calibrate Library. Check all of the data files and click Start under Peak Search. 382 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 383 The RSD of the curve varies depending upon the curve fit selected. Verify that the RSD of RF% is acceptable. It is recommended that the RSD of RF% is 30% or lower. 074-471-P1E HAPSITE ER Operating Manual 383 / 436...
Page 384 Click any number in the Conc. (Ratio to IS) column. The corresponding point is overlaid with a pink "X." Use the up and down arrows to select the outlying point. 384 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 385 INFICON Calibration | 16 Click Delete. If a point was inadvertently deleted, the original calibration points can be restored by clicking Restore Initial Record. 074-471-P1E HAPSITE ER Operating Manual 385 / 436...
Page 386 Click Search Settings to adjust all of the compounds at once. To adjust individual analytes, click View/Edit. To recalibrate the library with new parameters, check the Reset Library box. 386 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 387 INFICON Calibration | 16 Verify that the Peak Search is set to Search. Repeat Step 16 through Step 41. When the method is satisfactory, click Save Library. 074-471-P1E HAPSITE ER Operating Manual 387 / 436...
Page 388 16 | Calibration INFICON Click OK. Save the library to the method by clicking Save. 388 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 389 INFICON Calibration | 16 Click OK. 074-471-P1E HAPSITE ER Operating Manual 389 / 436...
Page 390: Definition Of Terms In The Calibrate Window
16 | Calibration INFICON 16.3 Definition of Terms in the Calibrate Window 390 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 391: Method
Recalculate Recalculates the peak areas and response factors without performing a peak search. This is most useful after manually editing the baseline points of the peak. Start Initiates the Search or Recalculation. 074-471-P1E HAPSITE ER Operating Manual 391 / 436...
Page 392: Analytes
Calibration Report that displays the response factor and curve statistics based Response on the selected curve type. Table Calibration Report that displays the area fit and purity for the calibration Report standards. 392 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 393 AMDIS or NIST library. Q Ion Shows the Quantitation Ion for the analyte. Ret. Time Shows the Retention Time for the analyte. Area Displays the integrated area of the quant ion. 074-471-P1E HAPSITE ER Operating Manual 393 / 436...
Page 394: Extracted Mass Peaks
This field is not used if the concentration flag is set to Global. 16.3.6 Extracted Mass Peaks Displays the mass peaks and relative percentages for the selected analyte. 16.3.7 Calibration Displays the calibration curve statistics for the selected analyte. 394 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 395: Build/Edit Template Menu
Verify that the Select Method First box is unchecked. Click OK. Select the desired method file. The data file that will be analyzed by ID Unknowns should have been generated from this file. Click OK. 074-471-P1E HAPSITE ER Operating Manual 395 / 436...
Page 396 16 | Calibration INFICON Click Browse in the Data Files section for the desired data file. Select the desired data file. 396 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 397 INFICON Calibration | 16 Click Start to open the Quant Report and the chromatogram. ð For information on reading the Quant Report, refer to View Reports [} 356]. 074-471-P1E HAPSITE ER Operating Manual 397 / 436...
Page 398 If the compound is not part of the calibration library, it is necessary to use AMDIS (refer to Analyzing Data Using AMDIS [} 222]) or NIST (NIST Library Searches [} 230]) to make an identification. 398 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 399: Definition Of Terms In The Id Unknowns Window
Displays the search parameter settings. Refer to Peak Search Settings Parameters [} 243]. Unknown Reports: By File Displays report by file. By Analyte Displays report by analyte 16.6.2 Data Files Browse Used to select the data file for analysis. 074-471-P1E HAPSITE ER Operating Manual 399 / 436...
Page 400: Peak Search
Shows the quantization ion for the analyte. Ret. Time Shows the retention time for the analyte. Area Displays the integrated area of the quant ion. Standard Designates the compound as an analyte or an internal standard. 400 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 401: Display Function
The Display button in the Data Files section of both Calibrate and ID Unknowns shows the chromatogram and spectrum of the selected data file. This feature is beneficial when reviewing and revising identifications, selecting spectral peaks, adding to a library and manually integrating peak areas. 074-471-P1E HAPSITE ER Operating Manual 401 / 436...
Page 402: Maintenance
17.2 HAPSITE Symptom - Cause - Remedy Chart Symptom Cause Remedy HAPSITE ER is not powering on. The battery is not charged. Verify that the battery is charged. Replace the power source if necessary.
Page 403 The probe is not recognized by The probe is not fully inserted into Insert the probe into the port until it HAPSITE ER, but appears to be the port. clicks into place. plugged in into the port.
Page 404 INFICON service personnel. WARNING Venting HAPSITE ER with a NEG that has not cooled causes total NEG consumption and may possibly result in severe damage to the HAPSITE ER mass spectrometer components. It may also result in physical injury since extreme heat generation from NEG consumption creates hot surfaces.
Page 405: Saturation Of The Probe And Probe Line
Blow out the probe line with nitrogen to remove any residual methanol that may be left in the probe line. Allow the probe to fully dry. Reattach the probe line to HAPSITE ER. WARNING Continue to wear PPE while blowing out probe and be sure that both ends of the probe are facing away from the user when blowing out occurs.
Page 406: Attaching Hapsite To The Service Module
NEG consumption creates hot surfaces. If HAPSITE ER is turned on with the NEG at 400°C, then the NEG must be cooled before proceding. Turn off HAPSITE ER and allow it to cool for approximately 24 hours or until the NEG is at room temperature.
Page 407 17.5.1 Attaching HAPSITE to the Service Module Using IQ Software Make sure that HAPSITE ER does not heat, or is in the NOT READY state. As soon as the HAPSITE ER screen is displayed, tap STOP PREPARE or using ◄▲▼►, highlight STOP PREPARE and tap OK SEL.
Page 408 CAUTION Prior to attaching HAPSITE ER to the Service Module, unplug the black NEG cable inside the HAPSITE ER front panel. This will ensure that the NEG will not heat when using the Service Module to provide vacuum. Connect HAPSITE ER to the computer using wireless communication on the crossover cable.
Page 409 0, the increases. After clicking Attach, the HAPSITE Properties window can be closed. ð The procedure typically takes about five minutes to complete. While attaching, the Attaching Service Module Please Wait message is displayed. 074-471-P1E HAPSITE ER Operating Manual 409 / 436...
Page 410 17.5.2 Attaching HAPSITE to the Service Module Using the HAPSITE Front Panel Controls To avoid running the start up method or AutoTune, tap STOP PREPARE. If using the push button keys, highlight STOP PREPARE with ◄▲▼ ►. Tap OK SEL. 410 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 411 INFICON Maintenance | 17 The SYSTEM IS NOT READY message will appear at the top of the screen. Tap the Accessory icon, or push the SYSTEM/STAT button until the accessory page appears. 074-471-P1E HAPSITE ER Operating Manual 411 / 436...
Page 412: Bakeout Procedure
A bakeout can be performed with or without the use of the Service Module. A bakeout heats the NEG pump to 700°C for a specified length of time. (The default time setting is two hours). Contact INFICON technical support before performing this procedure. Click on the Service Module tab of the Properties menu.
Page 413 INFICON Maintenance | 17 Click on the Service button. Verify that the settings match the setting displayed below and click Bakeout NEG. 074-471-P1E HAPSITE ER Operating Manual 413 / 436...
Page 414: Reactivating The Neg Pump
17 | Maintenance INFICON If the bakeout is complete, run a blank method. If the bakeout was successful HAPSITE ER will be operational. If the bakeout was unsuccessful, a high pressure error will occur. 17.6.1 Reactivating the NEG Pump Reactivating the NEG pump requires having the Service Module attached to HAPSITE ER for at least 22 hours.
Page 415 INFICON Maintenance | 17 Use the Activate NEG setting below as a guideline. However, change the HAPSITE ER Pumpdown Time to 180. Click the Activate NEG button. 074-471-P1E HAPSITE ER Operating Manual 415 / 436...
Page 416 17 | Maintenance INFICON At the end of the reactivation, the program will detach HAPSITE ER from the Service Module as part of the process. 416 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 417: Part Numbers
930-850-G7 230V HAPSITE ER United Kingdom 930-850-G8 230V HAPSITE ER Australia/China 930-206-G6 Hand control unit (Probe) 930-0371-G1 NIST and HAPSITE ER software only. No laptop. 930-261-G6 Laptop with Windows 930-261-G7 Ruggedized laptop with Windows 930-262-G6 Laptop with Windows (Europe) 930-262-G7...
Page 418: Hapsite Spare Parts
Concentrator Tubes: 930-251-G1 Tenax Kit 930-716-G1 Tri-Bed Kit Line Insulation: 070-1545 Probe insulation NIST: 930-4071-G1 NIST version upgrade 930-4081-G1 NIST (with AMDIS) Shipping Cases: 930-464-P1 HAPSITE ER 930-4131-P1 HAPSITE ER accessory case 418 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 419: Hapsite Consumables
HAPSITE Chemical Standards Kit, 19 part (for training and practice) 18.5 Service Module Spare Parts 068-0002 Battery charger/service module power cord, US. 930-0211-G1 Torque wrench kit 930-465-P1 Shipping case, service module 600-1001-P15 RS232 cable (15 ft.) 074-471-P1E HAPSITE ER Operating Manual 419 / 436...
Page 420: Glossary
Declaration of Contamination document. All chemicals that have been run through the HAPSITE ER must be listed on the form prior to service and repairs. Elution time The elapsed time from injection of a specific compound onto the column until the compound exits the column (same as retention time).
Page 421 Glossary | 19 Inlet state This refers to the valve states in the HAPSITE ER. The states of the valves control sampling, analysis, clean-out of the HAPSITE ER. Internal standard A mix of known compounds with known concentrations. They are mixed with the sample analytes to validate the response of the HAPSITE ER.
Page 422 Remote power Power supplied to the HAPSITE ER and Headspace sample system either from the Service Module (for the HAPSITE ER) or external AC - 24 V(dc) converter. Resolution These settings in the tune program affect the way the mass spectrometer resolves peaks.
Page 423 Turbo molecular pump Torr Unit of sub-atmospheric pressure. Equivalent to 133.3 pa. Vacuum The two-part valve which seals the HAPSITE ER manifold, when closed, and interconnect opens it to the vacuum pumps in the Service Module, when open. The vacuum valve interconnect valve is powered by a motor within the Service Module, under direction of the HAPSITE ER.
Page 424: Appendix A: Hapsite Target Compounds
1.61 79-01-6 cis-1,3-Dichloropropene CClH=CCClH2(H) g 2.08 542-75-6 4-Methyl-2-Pentanone CH3COCH2CH(CH3)CH3 g 2.11 43, 58 108-10-1 trans-1,3-Dichloropropene CClH=C(H)CClH2 2.44 542-75-6 1,1,2-Trichloroethane CHCl2CH2Cl 2.56 79-00-5 Toluene C6H5CH3 108-88-3 2-Hexanone CH3CO(CH2)3CH3 h 3.08 43, 58 591-78-6 424 / 436 074-471-P1E HAPSITE ER Operating Manual...
Page 425 C6H5CH=CH2 7.25 100-42-5 o-Xylene C6H4(CH3)2 j 7.52 1330-20-7 1,1,2,2-Tetrachloroethane CHCl2CHCl2 j 7.52 79-34-5 Note 1: 69, 75, 99, 125 Note 2: 79, 117, 167 k is the partition coefficient of a volatile. 074-471-P1E HAPSITE ER Operating Manual 425 / 436...
Page 426: Appendix B: Calibrating Gas Mixtures
1. How to establish the desired concentrations of the required compounds. See How to Establish the Desired Concentrations [} 426]. 2. Correct delivery of the mix to the inlet of the HAPSITE ER. See Correct Delivery of the Mix to the Inlet of the HAPSITE [} 428].
Page 427: Using Cylinders Containing Each Concentration
The method may specify, for example, 0.1 ppm, 1 ppm, and 10 ppm of each compound. Calibrate HAPSITE ER to bracket the concentrations at which the compounds of interest will occur in the samples.
Page 428: Diluting The Gas On-Site
The connection of the HAPSITE ER sample probe inlet should be at right angles to the direction of gas flow with 1/8 in. stainless steel Swagelok fittings.
Page 429: Inert Sample Bag
The smaller "leg" of the sampling tee is coupled to the HAPSITE ER. The total flow to the sampling tee should be approximately 1 liter/min. to allow sufficient excess over the HAPSITE ER sampling flow rate which is approximately 100 cc/min.
Page 430 Tedlar bag is removed. This will prevent the dropping of nuts and ferrules. The 1/8 in. end of the adapter is a swaged connection to the 1/8 in. male Swagelok fitting on the end of the HAPSITE ER probe, so wrenches will be required to make a leak free connection.
Page 431 40 liter Tedlar bag. Larger Tedlar bags are available, but convenience in regular handling and the possibility of target compound adsorption on the larger interior surface area may be matters of concern. 074-471-P1E HAPSITE ER Operating Manual 431 / 436...
Page 432: Appendix C: Shipping The Hapsite And Consumables
Consumables 22.1 Introduction The HAPSITE ER instrument is designed to ship to remote locations. The instrument can be reshipped in the cardboard boxes (with the same cut-foam inserts) in which it is received. However, these boxes will not suffice for frequent shipping. A heavy-duty fitted shipping case for HAPSITE ER is available from INFICON (part number 930-464-P1).
Page 433 WARNING Do not ship canisters installed in the HAPSITE ER; they are still hazardous and can damage the unit during transport. The regulations governing shipments of hazardous goods are found in the DOT portion of the Code of Federal Regulations: Part 171, 172 and 173 of 49 C.F.R.
Page 434: Empty Canisters
Mark the box as "Empty Canisters for Destruction." Ship them, prepaid, to: Airgas USA, LLC 259 North Radnor-Chester Road Suite 100, Radnor, PA 19087-5283 1-866-734-2338. 434 / 436 074-471-P1E HAPSITE ER Operating Manual...

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