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Labnet BioPette E BE20-1 series Operation Manual

Single and 8-channel electronic pipettes
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BioPette E
Single and 8-channel Electronic Pipettes
Operation Manual
Version: 2.7
www.labnetlink.com

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Summary of Contents for Labnet BioPette E BE20-1 series

  • Page 1 BioPette E Single and 8-channel Electronic Pipettes Operation Manual Version: 2.7 www.labnetlink.com...
  • Page 3 No part of this manual may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, form or by any means, electronic or mechanical, for any purpose, without prior written permission of Labnet International, Inc. without prior written permission of Labnet International, Inc.

  • Page 4: Table Of Contents

    Table of Contents Safety Precautions...................1 1.1. Operation Safety Precautions ............1 1.2. Battery Safety ..................1 General Description ................3 2.1. Features....................3 2.2. Ranges and Specifications ...............4 Getting Started ..................4 3.1. Unpacking..................4 3.2. Inserting the Battery ................5 3.3. Charging the Battery................6 3.4. Start Pipetting ..................8 3.5.
  • Page 5 MD (Multiple Dispensing) Mode ............20 MIX Mode SE (Sequential Aspirating/Dispensing) Mode ........22 10.1. SA (Sequential Aspirating) Mode – Not available on BE1000-1 because the diameter of 1000ul tips does not allow the required air gap to be maintained....................22 10.2. SD (Sequential Dispensing) Mode ..........24 Sterilization 26 11.1.

  • Page 6: Safety Precautions

    2. When using organic solvents or corrosive chemicals, please check their compatibility with the pipette tips and the BioPette E. 3. Repair should be carried out by Labnet authorized service personnel only. Contact Labnet International, Inc. for information on authorized service options.
  • Page 7 3. Do not incinerate the Lithium battery or expose it to excessive heat. 4. Do not short-circuit, puncture, crush, disassemble, damage, force over-discharge (reversal) or modify the battery. 5. Do not expose the battery to water or moisture. 6. Do not drop or subject the battery to strong impacts. 7.

  • Page 8: General Description

    2. General Description Labnet's BioPette E is designed to replace the traditional manual pipette. It features an accurate and efficient, as well as ergonomic and light-weight design. Researchers can program this state-of-the-art product to conduct different pipetting tasks. More accurate and comfortable pipetting can be easily achieved without any concern of finger stress or injury.

  • Page 9: Ranges And Specifications

    X: power plug, no designation=U.S. plug, EU=European plug, UK=U.K. plug, AU=Australian plug BioPette E Accuracy and Precision Specifications have been attained using Labnet brand pipette tips. Labnet guarantees Accuracy and Precision only if Labnet brand tips or other approved tips are used. 3. Getting Started 3.1. Unpacking...

  • Page 10: Inserting The Battery

    If there are any items missing, damaged, or not according to your order, please contact your distributor or sales representatives for replacement immediately. Please charge the battery before first-time operation. 3.2. Inserting the Battery Remove the battery cover by pressing the latch (see Figure 1). Insert the battery (see Figure 2).

  • Page 11: Charging The Battery

    3.3. Charging the Battery There are two ways to charge the battery: (1) Use BioPette E’s internal charging circuit, (2) Use the optional charger. BioPette E’s Internal Charging Circuit Insert the battery into the battery compartment. Insert the DC-in plug of the power adaptor into the DC-in jack (see Figure 3).
  • Page 12 Figure 3: Using BioPette E’s internal Figure 4: Using the optional charging circuit to charge the battery charger to charge the battery Note: 1. For safety reasons, the BioPette E cannot be used during charging. 2. Please do not over-charge the battery for a long period of time.

  • Page 13: Start Pipetting

    3.4. Start Pipetting There is no power On/Off button on the BioPette E. Once users insert the fully-charged battery, the BioPette E will be switched on. It will perform a calibration routine and switch on the LCD display as in Figure 8. Press any keypad button or the button to enter the function mode selection.

  • Page 14: Hanger Installation

    3.5. Hanger Installation The BioPette E hanger can be attached to a shelf or counter using the screw or sticker as shown in Figure 6. The finger rest of the BioPette E locks into the hanger and keeps the BioPette E in a vertical position. Figure 6: BioPette E hanger installation 4.
  • Page 15 LCD Display Keypad Battery C "RESET" Tip Ejector "PLUNG Finger R Bushing Connecti Lower Part Cone (Front View) (Side View) Figure 1 : Overview Figure 7: 1-channel BioPette E overview...

  • Page 18: Keypad Function

    Figure 8: LCD display Panel 4.1. Keypad Function Button Symbol Function Description Set/Enter Confirms your choice Exit/Esc Exits to previous mode/setting Increases volume/ changes parameter Down Decreases volume/ changes parameter Piston automatically determines the Reset RESET home position Activates aspirating/dispensing/ PLUNGER PLUNGER same as...
  • Page 19 Audible Sound Operation Low-tone beep Aspirating High-tone beep Dispensing/ Tip Ejection/ Error codes MD/MIX/SE mode cycle or RESET routine is 2 beeps completed...

  • Page 20: Function Map

    5. Function Map The main menu of the BioPette E includes five function modes (SYS, AUTO, MD, MIX and SE). Each mode includes various function settings (see Figure 9). Function Map Function AUTO Mode Basic Dispensing Aspirating Aspirating/ Number Number Speed Dispensing (COUNT)

  • Page 21: Sys (System) Mode

    6. SYS (System) Mode SYS mode is used to set up the function settings before pipetting. Once users select and store these settings, they will no longer need to set up the settings before every pipetting. There are 7 function settings (Aspirating Speed, Dispensing Speed, Audible Signals, Tip Ejection Alarm, Blow-out, Reverse Pipetting and Program Recall) in SYS mode.

  • Page 22: Dispensing Speed

    6.2. Dispensing Speed 1. The “Dispensing Speed” symbol should now be flashing. 2. Press «Up/Down» to change the aspirating speed ( : Slowest, Fastest). 3. Press «SET» to confirm the speed selection and move to the “Audible Signals” function setting. 6.3.

  • Page 23: Blow-Out

    6.5. Blow-out The automatic blow-out function, in different pipetting modes, simulates the blow-out function in manual pipettes. To avoid the leftover final droplet in tips, the blow-out setting is recommended in all protocols. The blow-out setting will not be performed in “Reverse Pipetting” setting. 1.

  • Page 24: Setting Prog (Programs)

    Note: If Reverse Pipetting is selected, users have to press the PLUNGER button one more time after pipetting to dispense the remaining liquid during the pipetting. will appear on the LCD to remind users to dispense the remaining liquid. 6.7. Setting PROG (Programs) The memory program includes 9 different storage locations (PROG 01 ~ 09).

  • Page 25: Auto Mode

    Note: During the recall process, the changes of other settings will not be stored. 6. AUTO Mode In AUTO mode, the BioPette E performs the aspirating and dispensing of a set liquid volume. 1. Press «EXIT» to enter the mode selection, which causes the symbol to start flashing.

  • Page 26: Mix Mode

    3. Press «SET» to confirm the selection. The symbol will then appear. 4. Use «Up/Down» to select the desired pipetting count. 5. Press «SET» to confirm the selection of count. The default pipetting volume will start flashing. 6. Use «Up/Down» to select the desired pipetting volume. 7.

  • Page 27: Se (Sequential Aspirating/Dispensing) Mode

    3. Press «SET» to confirm the selection. The symbol will then appear. 4. Use «Up/Down» to select the desired mixing count. 5. Press «SET» to confirm the selection. The default pipetting volume will start flashing. 6. Use «Up/Down» to select the desired mixing volume. 7.
  • Page 28 4. Use «Up/Down» until the symbol starts flashing. 5. Press «SET» to confirm the selection. 6. Under the symbol, “01” will appear. Press «Up/Down» to set the volume of the first aspiration. 7. Press «SET» to confirm the selection. 8. Repeat steps 6 and 7 to set the sequential aspiration volumes. The total aspirating volume (including air gap volume) cannot exceed maximum volume range.

  • Page 29: Sd (Sequential Dispensing) Mode

    2. A1000 models do not include the Sequential Aspirating (SA) function, because the air gap is not strong enough to support the weight of samples. 3. Sequential Aspirating (SA) function will not perform Reverse Pipetting even it is selected in System (SYS) mode. 10.2.
  • Page 30 aspirate the sample. When the aspiration is completed, the dispensing count and volume will appear and will flash to indicate that the BioPette E is ready for dispensing. 11. The BioPette E will make two beeping sounds to remind the user of the last measured volume to dispense.

  • Page 31: Sterilization

    10. Sterilization Only the lower piston assembly of BioPette E can be steam-autoclaved (121 C, 1 bar, 20 minutes). NOTE: It is important not to exceed the the temperature of 121C or 20 minute time during the autoclaving cycle. Autoclaving at a higher temperature or for longer period of time will damage the manifold and void the warranty.

  • Page 32: Removal Of The Lower Piston Assembly

    11.1. Removal of the Lower Piston Assembly RESET Always remember to press button before you disassemble the lower part of BioPette E. RESET 1. Press the button. Wait until you hear two beeps. 2. Loosen the lower part of BioPette E by unscrewing the connecting nut in counter-clockwise direction as shown in Figure 11.

  • Page 33: Reattach The Lower Piston Assembly

    Cylinder lock Figure 14: The lower piston Figure 13: Push the metal cylinder lock assembly will come off easily and downward until you hear a click sound. automatically. 11.2. Reattach the lower piston assembly RESET Always remember to press button after you remove the lower piston assembly of BioPette E.

  • Page 34: Maintenance

    Lubrication of the piston should be performed only by authorized Labnet service personnel, or trained users, and only the proper BioPette E lubricant available from Labnet International should be used*.

  • Page 35: Troubleshooting

    If the following solutions are not able to resolve the problem, please contact Labnet International’s Service Department to arrange for authorized service. It is also recommended to check Labnet International’s web site at www.labnetlink.com for useful and current technical information.
  • Page 36 Tip incorrectly attached Attach firmly Unsuitable tip Use high-quality tips Failure to aspirate Low battery Charge the battery The lower manifold is not Detach and correctly attached reassemble Foreign material blocking Use MIX mode the hole at bottom of the and distilled cone water to wash.

  • Page 37: Appendix A: Technical Specifications

    Appendix A: Technical Specifications Automatic Pipetting (AUTO), Multiple Dispensing (MD), Mixing (MIX), Operation Mode Sequential Aspirating/Dispensing (SE), System Setup (SYS) Program memory 9 programs 5 speeds Aspirating/Dispensing Speed Auto-calibration Piston Motor High precision stepping motor Yes, after 10 minutes Power Saving Feature Power Adaptor 100/120/220/240 V AC-DC 3.6 V Audible Alerts...

  • Page 38: Appendix C: Warranty

    Figure 15 Tip ejector height adjustment Appendix C: Warranty Labnet’s BioPette E electronic pipettes are covered by a warranty for one year against defects in materials and workmanship. This period begins from the date of purchase, and within this period all defective parts will be replaced at no charge by Labnet International.
  • Page 39 Labnet International Inc., PO Box 841, Woodbridge, NJ 08837 TEL: +732-417-0700 FAX: +732-417-1750 US Toll Free: 888-522-6381 E-mail: Labnet@labnetlink.com www.labnetlink.com Web Site:...

This manual is also suitable for:

Biopette e be200-1 seriesBiopette e be1000-1 seriesBiopette e be20-8 seriesBiopette e be200-8 series

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