Beckman Coulter SX4750 Instructions For Use Manual
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Beckman Coulter SX4750 Instructions For Use Manual

Beckman Coulter SX4750 Instructions For Use Manual

Swinging-bucket rotor
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Instructions For Use
SX4750 Swinging-Bucket Rotor
For Use in the Beckman Coulter
Allegra X-12 Series,
Allegra X-14 Series, and
Allegra X-15R Centrifuges
GX-TB-003FD
February 2023
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

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  • Page 1 Instructions For Use SX4750 Swinging-Bucket Rotor For Use in the Beckman Coulter Allegra X-12 Series, Allegra X-14 Series, and Allegra X-15R Centrifuges GX-TB-003FD February 2023 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
  • Page 2 • In the UK, call us at +44 845 600 1345 • In Ireland, call us at +353 (01) 4073082 • In Italy, call us at +39 0295392 456 • In other locales, contact your local Beckman Coulter Representative. Find us on the World Wide Web at: www.beckman.com...

  • Page 3: Revision History

    Issue FB, 02/2014 The following section has changed subsequent to revision FA: • Table 2, Beckman Coulter Tubes and Bottles for the SX4750 RotorBeckman Coulter, Inc. • SX4750 Warranty Issue FC, 02/2018 The following section has changed subsequent to revision FB: •...
  • Page 4 Revision History GX-TB-003FD...

  • Page 5: Safety Notice

    Contact Beckman Coulter, Inc. urges its customers and employees to comply with all national health and safety standards such as the use of barrier protection. This may include, but is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other laboratory centrifuge.

  • Page 6: Alerts For Warning, Caution, And Note

    This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Their safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval.

  • Page 7: Safety During Installation And/Or Maintenance

    Beckman Coulter Representative or returning parts to Beckman Coulter for repair. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. If any parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated.

  • Page 8: Radiation Safety

    Refer to applicable external device user’s manuals for operating procedures of that device. NOTE For Safety Data Sheets (SDS) information, go to the Beckman Coulter website at https://www.beckman.com/techdocs. WARNING Risk of chemical injury from bleach. To avoid contact with the bleach, use barrie protection, including protective eyewear, gloves, and suitable laboratory attire.

  • Page 9: Mechanical Safety

    Safety Notice Mechanical Safety WARNING Risk of personal injury or property damage. The rotors and accessories are not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials in, nor handle or store them near the centrifuge.

  • Page 10: Maintenance

    Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all of your rotors for corrosion or damage.

  • Page 11: Table Of Contents

    Contents Revision History, iii Safety Notice, v Alerts for Warning, Caution, and Note, vi Safety Information for the SX4750 Rotor, vi Safety During Installation and/or Maintenance, vii Radiation Safety, viii Chemical and Biological Safety, viii Mechanical Safety, ix Cleaning, x...
  • Page 12 Cell Culture Flask Adapters, 27 Decontamination, 27 Sterilization and Disinfection, 28 Tube Breakage, 28 Aerosolve Cannisters, 29 SST or Corvac Tubes, 29 Storage, 30 Returning a Rotor, 30 Supply List, 31 Replacement Rotor Parts, 31 Other, 31 Beckman Coulter, Inc. SX4750 Warranty...
  • Page 13 Illustrations Illustrations The SX4750 Rotor and Accessories, 2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers, 9 Assembling a Modular Disk Adapter, 10 Blood Bag Loading Procedures, 12 Lubricating Pivot Pin/Bucket Contact Areas, 26 xiii...
  • Page 14 Tables Tables Adapters and Blood Bag Cups Available for the SX4750 Rotor, 17 Beckman Coulter Tubes and Bottles for the SX4750 Rotor, 19 Beckman Coulter Labware for Use with Multiwell-Plate Carriers, 23 Commercially Available Labware for Use with Multiwell-Plate Carriers, 24...

  • Page 15: Sx4750

    SX4750 Swinging-Bucket Rotor Specifications Maximum speed Allegra X-15R (tube-and-bottle buckets) ......4750 RPM Allegra X-15R (multiwell-plate carriers)......4450 RPM Allegra X-14 series (tube-and-bottle buckets) ....4300 RPM Allegra X-14 series (multiwell-plate carriers)....4000 RPM Allegra X-12 series (all buckets) ........... 3750 RPM Critical speed range ............

  • Page 16: Description

    5. Carriage 3. Bottle This Beckman Coulter rotor has been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges. The SX4750 is a four-place swinging-bucket rotor. The rotor buckets carry adapters that allow centrifugation of a wide range of tube and bottle sizes (from 1.5 mL reaction vials to 750-mL bottles)

  • Page 17: Buckets And Accessories

    Covers have been tested to demonstrate containment of microbiological aerosols under normal conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed. The covers, which are held in place by attached latch assemblies, will...

  • Page 18: Bottle Adapters

    3. Aerosolve Cannister Aerosolve cannisters, designed to contain aerosol leakage and liquid spills, can be used in the SX4750 buckets when this additional containment is required. The cannister was tested demonstrate containment of microbiological aerosols under normal operating conditions of the * Validation of microbiological containment was done at an independent third-party testing facility (Public Health England).

  • Page 19: Cell Culture Flask Adapters

    SX4750 Swinging-Bucket Rotor Description associated Beckman Coulter centrifuge, when used and maintained as instructed. Aerosolve cannisters hold a variety of tube sizes in racks, or they can be used as 500-mL wide-mouth bottles. CAUTION Do not run chloroformed samples in Aerosolve cannisters. Chloroform vapors can damage the cannister material.

  • Page 20: Run Preparation

    Run Preparation Run Preparation Specific information about the SX4750 rotor is given here. Information about the use and care of the centrifuge is contained in the centrifuge manual, which should be used together with this manual for complete rotor and centrifuge operation.

  • Page 21: Loading The Buckets

    Symmetrical and Balanced Loading Beckman Coulter supplies buckets and carriers in weight-matched sets to make balancing easier (the weight and retirement date are marked on each bucket and carrier). Modular disk adapters are also sold in weight-matched sets. However, there are variances in weight between sets, as well as variance in weight between previously purchased adapters.

  • Page 22: Using Tube And Bottle Buckets

    SX4750 Swinging-Bucket Rotor Loading the Buckets For best results, load opposing buckets or carriers with the same type of labware containing the same amounts of fluid of equal density. Additionally, opposing buckets or carriers and their contents should weigh approximately the same (within 6 grams). Do not exceed the rated maximum load for buckets (1000 grams each for tube-and-bottle buckets;...

  • Page 23: Examples Of Correctly And Incorrectly Loaded Buckets And Carriers

    SX4750 Swinging-Bucket Rotor Loading the Buckets Figure 2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers Balanced Load Unbalanced Load CORRECT INCORRECT 1. Balanced Load 5. CORRECT 2. Unbalanced Load 6. Examples of Symmetrically Loaded Multiwell Plates (load opposite plates the same way) 3.

  • Page 24: Using Tubes

    SX4750 Swinging-Bucket Rotor Loading the Buckets Using Tubes Assembling Modular Disk Adapters Refer to Table 1 to determine which color adapter is required for the tubes you are using and the number of disks needed to support the length of the tubes.

  • Page 25: Loading Tubes In Adapters

    SX4750 Swinging-Bucket Rotor Loading the Buckets Loading Tubes in Adapters When placing tubes in modular disk adapters, it is important to make sure that they will not contact the rotor yoke during a run. • One way to do this is to place empty tubes in an adapter, place the adapter in a bucket on the rotor yoke, and manually swing the bucket to the horizontal position, making sure that all tubes clear the yoke.

  • Page 26: Using Blood Bag Cups

    SX4750 Swinging-Bucket Rotor Loading the Buckets provide support and prevent the cannisters from being damaged during centrifugation. These pads are included in the Aerosolve cannister kits (359232). Bucket covers cannot be used with Aerosolve cannisters. Using Blood Bag Cups NOTE This rotor is suitable for obtaining cell-free plasma or for cell packing.

  • Page 27: Using Cell Culture Flask Adapters

    SX4750 Swinging-Bucket Rotor Loading the Buckets Make sure the loaded blood bag cups opposite each other on the rotor yoke are approximately the same weight (within 1 gram). • Soft, pliable balancing pads that will not puncture blood bags (see the...

  • Page 28: Loading Buckets Onto The Yoke

    SX4750 Swinging-Bucket Rotor Loading the Buckets Make sure that the O-ring (961648) is in good condition and lightly coated with silicone vacuum grease (335148). • Place the O-ring on the ledge inside the bucket. 1. Insert O-ring here Place the cover on the bucket with the latches perpendicular to the pin sockets.
  • Page 29 SX4750 Swinging-Bucket Rotor Loading the Buckets Insert the filled plate(s) into the carriage. • If using two or three Beckman Coulter plates per carrier, place a 96-well cap strip (see Table 3) between the plates to prevent breakage during centrifugation. NOTE Up to three Beckman Coulter plates, separated by cap strips, can be run per carrier at maximum multiwell-plate speeds.

  • Page 30: Operation

    Coulter multiwell plates are shown in Table Temperature Limits • Beckman Coulter plastic tubes and bottles have been centrifuge tested for use at temperatures between 2 and 25°C. For centrifugation at other temperatures, pretest tubes under anticipated run conditions.

  • Page 31: Adapter

    SX4750 Swinging-Bucket Rotor Operation Table 1 Adapters and Blood Bag Cups Available for the SX4750 Rotor MODULAR DISK ADAPTERS (polypropylene) Color Nom. Nom. Max. No. Max. No. RCF at Adapter Part No. Code Tube Tube Tubes per Tubes in Maximum...
  • Page 32 SX4750 Swinging-Bucket Rotor Operation CELL CULTURE FLASK ADAPTERS (EPDM) Color Code ) Number Flasks at Flask RCF at Max mum Part Number (qty Flask Size (cm per Adapter Speed ( g) two) Bottom (mm) orange 195.3 2080 369292 green 177.5...

  • Page 33: Blue

    Tube racks used with Aerosolve cannisters do not provide full tube support; some manufacturers’ plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks. Beckman Coulter highly recommends that you pretest other manufacturers’...
  • Page 34 SX4750 Swinging-Bucket Rotor Operation Table 2 Beckman Coulter Tubes and Bottles for the SX4750 Rotor (Continued) OPEN-TOP TUBES Dimensions Volume Description Part Number Adapter (mL) Set of Two Set of Four 18  98 mm polypropylene 342082 359473 359152 29  104 mm...
  • Page 35 SX4750 Swinging-Bucket Rotor Operation Table 2 Beckman Coulter Tubes and Bottles for the SX4750 Rotor (Continued) OPEN-TOP TUBES Dimensions Volume Description Part Number Adapter (mL) Set of Two Set of Four 18  98 mm polypropylene 342082 359473 359152 29  104 mm...
  • Page 36 SX4750 Swinging-Bucket Rotor Operation b. Adapter used in Aerosolve cannister (part number 359232). c. Tube retainers and adapter plates are sold individually. CONICAL TUBES Dimensions Volume Description Part Number Adapter (mL) Set of Two Set of Four 62  141 mm...
  • Page 37 These tubes cannot be run with bucket covers in place. d. Replacement bottle cap part number is 356263 (set of 6). e. Do not load the 750-mL bottle directly into the bucket; always use the adapter. Table 3 Beckman Coulter Labware for Use with Multiwell-Plate Carriers Description Volume...

  • Page 38: Run Speeds

    • 3750 RPM for either buckets or carriers in an Allegra X-12 series centrifuge. (Up to three Beckman Coulter plates can be run per carrier at maximum multiwell-plate speeds. If running four Beckman Coulter plates, reduce run speed to 2700 RPM. Observe manufacturer’s run speed and stacking limitations if using other manufacturers’...

  • Page 39: Care And Maintenance

    • If any of these signs are evident, do not run the rotor. • Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center. Approximately every 400 runs, and after cleaning and/or autoclaving, wipe the rotor pins and...

  • Page 40: Cleaning

    Store the rotor in a dry environment (not in the centrifuge). Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center.

  • Page 41: Modular Disk Adapters

    If aluminum rotor components become contaminated with radioactive material, decontaminate them using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or †...

  • Page 42: Sterilization And Disinfection

    SX4750 Swinging-Bucket Rotor Care and Maintenance While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use.

  • Page 43: Aerosolve Cannisters

    SX4750 Swinging-Bucket Rotor Care and Maintenance Aerosolve Cannisters WARNING When working with potentially hazardous materials, open cannisters in an appropriate hood or biological safety cabinet in case of tube breakage. If a glass tube breaks in an Aerosolve cannister tube rack, discard and replace the O-ring and rubber tube cushion in the base of the rack.

  • Page 44: Storage

    Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. This form may be obtained from your local Beckman Coulter sales office. The form, entitled Returned Material Authorization (RMA) for United States returns or Returned Goods Authorization (RGA) for international returns, should contain the following information: •...

  • Page 45: Supply List

    Publications referenced in this manual can be obtained at www.beckman.com or by calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office. Call Beckman Coulter Sales (1-800-742-2345 in the United States) for detailed information on ordering parts and supplies.
  • Page 46 SX4750 Swinging-Bucket Rotor Supply List Neoprene tube cushions for blue rack (pkg of 24) ........... 344117 for orange rack (pkg of 18) ..........344118 for purple rack (pkg of 18; 12 large, 6 small) ....344119 for dark green rack (pkg of 18; 12 large, 6 small) ...

  • Page 47: Beckman Coulter, Inc

    Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery...
  • Page 48 Beckman Coulter, Inc. SX4750 Warranty Warranty-2 GX-TB-003FD...

  • Page 50: Related Documents

    Centrifugation Products (IN-175) Use and Care of Tubes and Bottles (IN-192) • Using Adapters for Corning 25-cm Cell • Culture Flask in SX4750 and SX4750A Rotors (GX-TB-007) Using Adapters for Corning 75-cm Cell • Culture Flask in SX4750 and SX4750A Rotors...

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