Thermo Scientific Applied Biosystems ProFlex PCR System Quick Reference page 4

Connect by mobile device
1. Download the "Instrument Connect Mobile Application" on
your mobile device.
™
a. For iPad or iPhone
application from the iTunes
for Instrument Connect by Thermo Fisher Scientific.
b. For Android devices, download the application from
™
Google Play by searching for Instrument Connect by
Thermo Fisher Scientific.
2. Launch the Instrument Connect Mobile Application and log
in using your Connect login and password.
3. Capture the QR code on the instrument screen.
How to use the ProFlex
™
Operate the ProFlex Thermal Cycler using the touchscreen. For
detailed instructions on using the instrument, refer to the ProFlex
PCR System User Guide (Pub. No. MAN0007697), available at
thermofisher.com/proflex
From the instrument Home screen, you can set up and run a new
Method using the following instructions:
1. Press New Method or Set Up Run on the status dial of an
available block.
2. Select a Method template.
3. Edit the parameters of the template such as temperature,
time, number of steps/stages.
4. For advanced editing options, press Manage Steps >
Advanced Options. Advanced editing options include:
™
VeriFlex for optimization, simulation modes, ramp rates,
and AutoDelta.
5. Save the new Method to a folder or directly start a run by
pressing Start Run.
4
™
devices, download the
™
music store by searching
Thermal Cycler
™
Note: For the ProFlex
to choose a block prior to starting a run.
Maintenance guidelines
CAUTION!
of the heated cover can be as high as 110°C, and the
temperature of the sample block(s) can be as high as
100°C. Before performing the procedure, keep hands away
until the heated cover and sample block(s) reach room
temperature.
To ensure proper operation:
• Regularly:
– Wipe the instrument surfaces with a lint-free cloth.
– Clean the vents, touchscreen, and sample block of the
instrument.
– Clean the sample wells with 100% isopropanol.
• Use only consumables recommended by Thermo Fisher
Scientific for the instrument.
Use of consumables that are larger or smaller than the
specified volume can damage the instrument, contaminate
the sample block, and/or decrease the PCR yield (due to
inefficient thermal transfer).
• Do not use sharp objects on the touchscreen.
Use only your fingers or blunt objects to enter commands
on the instrument touchscreen. Sharp and/or pointed objects
such as writing utensils can damage the surface of the
touchscreen.
• Back up frequently.
Routinely back up the configurations and files on your
instrument to a USB drive. Regular backups protect against
data loss caused by user error, power failure, or instrument
error. For more information, see the ProFlex
User Guide (Pub. No. MAN0007697).
How to use the MicroAmp
IMPORTANT!
The retainer is essential for optimal performance,
and its usage is recommended at all times for the ProFlex
™
3 × 32‑Well PCR System. The retainer allows pressure to be
distributed evenly over the tubes to ensure a proper seal, and
prevent tubes from being crushed or deformed.
The retainer is compatible for use with MicroAmp
strips.
Figure 8 MicroAmp
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ProFlex PCR System Installation and Operation Quick Reference
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3x32 Well PCR System, you will need
During instrument operation, the temperature
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3 × 32-Well Retainer
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3x32-Well Retainer
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PCR System
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tubes and tube