Gel Pouring - Labnet ENDURO96 E1010-9611 User Manual

until all crystals are dissolved. This is best viewed against a light
background. Crystals appear as translucent crystals. These will interfere
with sample migration if not completely dissolved.
The gel must be cooled to between 50°C and 60°C degrees before

Gel Pouring:-

The range of units allows three different methods of gel casting:-
Casting Dams
Flexicaster
Traditional Tape
Using trays with Casting Dams:
1. Fit the casting dams over each end of the tray and place onto a level
surface. The dams should be fitted so that there is no gap between the
sides of the tray and the groove in the dams. This will ensure that
there is no possibility of gel leakage.
2. Place the comb(s) in the grooves. Each tray has more than one comb
grove so that multiple combs can be used. Using multiple combs
increases sample number available per gel but decreases run length
and care must be taken to ensure that samples from the first wells do
not migrate into the lanes of the second comb wells.
3. Pour in the agarose carefully so as not to generate bubbles. Any
bubbles that do occur can be smoothed to the edge of the gel and
dispersed using a pipette tip.
4. Allow the agarose to set, ensuring that the gel remains undisturbed.
5. Carefully remove the gel casting gates and comb and transfer the gel
including tray to the main tank.
pouring.
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