Solutions; Combs - Labnet ENDURO96 E1010-9611 User Manual

2. Current Protocols in Molecular Biology, Greene Publishing Associates
and Wiley-Interscience,1989.

Solutions:-

1x TAE 40 mMtris (pH 7.6), 20 mM acetic acid, 1 mM EDTA.
50x (1L) dissolve in 750 ml distilled water:
242 g tris base (FW = 121)
57.1 ml glacial acetic acid
100 ml 0.5 M EDTA (pH 8.0).
Fill to 1 litre with distilled water.
1x TBE 89 mMtris (pH 7.6), 89 mM boric acid, 2 mM EDTA
10x (1L) dissolve in 750 ml distilled water:
108 g tris base (FW = 121)
55 g boric acid (FW = 61.8)
40 ml 0.5 M EDTA (pH 8.0)
Fill to 1 litre with distilled water.
Sample Loading Dye
10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue,
and
0.25% xylene cyanole FF in 1x TAE buffer. Only 1–10 ml of the 10x loading
dye should be prepared.
Ethidium Bromide Solution
Add 10 mg of Ethidium Bromideto 1 ml distilled water.
Comb blocks– MC Denotes Multi Channel Pipette compatible.
Description
96 Comb 8 sample MC + 1 Marker, 1mm thick
COMB BLOCK
96 Comb 8 sample MC + 1 Marker, 1.5mm
thick COMB BLOCK
Part Number
E1010-9611-CB
E1010-9611.5-CB
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