Gel Staining - Labnet ENDURO96 E1010-9611 User Manual

4. Once loaded, gently immerse the gels within the buffer, stacking them
carefully on top of each other. Replace lid and connect unit to the power
supply using cables.
5. Typically gels are run at between 90 and 150 volts. However, maximum
voltages are indicated on the serial badge of each unit. It should be noted
that higher voltages generally give faster but poorer quality sample
resolution.
Gel Staining and Viewing:-
The Multi Sub trays and the Mini Fast unit allow staining to be performed
without removing the gel from the tray if this is preferred.
1. Transfer the gel to a vessel containing the appropriate volume of 0.5
µg/ml ethidium bromide stain for 15–30 minutes, see solutions for stock
stain concentration and adjust to the volume used accordingly. The entire
gel should be covered.
NOTE:- Ethidium bromide is a suspected carcinogen and the necessary
safety precautions should be undertaken.
2. De-stain the gel for 10–30 minutes in distilled water again ensuring the
gel is completely immersed.
3. Rinse the gel twice for a couple of seconds with distilled water.
4. Transfer the gel to a UV Transilluminator.
5. The samples will often appear as brighter, clearer bands when
photographed or viewed using a gel documentation system. However if the
gel bands are too faint then the staining procedure should be adjusted so
that there is less de-staining. If there is too much background then the
staining procedure should be adjusted so that there is more de-staining.
References
1. Sambrook, Fritsch, and Maniatis, Molecular Cloning A Laboratory
Manual, Second Edition,
Cold Spring Harbor Laboratory Press, 1989.
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