Related Manuals for Labnet ENDURO96 E1010-9611
Summary of Contents for Labnet ENDURO96 E1010-9611
- Page 1 ENDURO96 HORIZONTAL GEL BOXES User Manual E1010-9611 E1010-9611.5 E1010-9621 E1010-9621.5 Lit M00203 May 2012...
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Page 2: Table Of Contents
Contents:- Page Safety Instructions Packing Lists Usage Guidance and restrictions Care and Maintenance Setting Up Gel Preparation Gel Pouring Gel Running Gel Staining Solutions Combs Symbols & Conventions Equipment Disposal Declaration of Conformity Warranty Lit M00203 May 2012... -
Page 3: Safety Instructions
SAFETY PRECAUTION WHEN USED CORRECTLY, THESE UNITS POSE NO HEALTH RISK. HOWEVER, THESE UNITS CAN DELIVER DANGEROUS LEVELS OF ELECTRICITY AND ARE TO BE OPERATED ONLY BY QUALIFIED PERSONNEL FOLLOWING THE GUIDELINES LAID OUT IN THIS INSTRUCTION MANUAL. ANYONE INTENDING TO USE THIS EQUIPMENT SHOULD READ THE COMPLETE MANUAL THOROUGHLY. -
Page 4: Packing Lists
PACKING LISTS: E1010-9611, E1010-9611.5, E1010-9621, E1010-9621.5 Units include tank, lid and electrodes and include the following accessories:- Combs and Cat no. Tray Tray Cables comb block Dams E1010-96T 10 x E1010-CD E1010-9611-CB E1107-EP E1010-9611 12cm (W x L) Pack of 2 E1010-96T 10 x E1010-CD E1010-9611.5-CB... -
Page 5: Usage Guidance And Restrictions
Usage Guidance and restrictions: • Maximum altitude 2,000m. • Temperature range between 4°C and 65°C. • Maximum relative humidity 80% for temperatures up to 31 C decreasing linearly to 50%relative humidity at 40 • Not for outdoor Use. This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664. POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs. -
Page 6: Setting Up
Acetone, Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Isopropyl alcohol Alkalis. Rnase Decontamination This can be performed using the following protocol:- Clean the units with a mild detergent as described above. Wash with 3% hydrogen peroxide (H2O2) for 10 minutes. Rinsed with 0.1% DEPC- (diethyl pyrocarbonate) treated distilled water, Caution: DEPC is a suspected carcinogen. -
Page 7: Gel Preparation
Instructions for fitting Loading Guides These can be fitted to enhance visibility of the wells if desired. They can be fitted to the white vinyl platform sheet or to the unit itself. 1. Seat the tray in the unit and note the position of the comb grooves. The samples run black to red but the trays can be used frontward or backwards so ensure that the comb grooves closest to the black electrode are marked. -
Page 8: Gel Pouring
until all crystals are dissolved. This is best viewed against a light background. Crystals appear as translucent crystals. These will interfere with sample migration if not completely dissolved. The gel must be cooled to between 50°C and 60°C degrees before pouring. -
Page 9: Gel Running
Using Traditional tape method:- 1. Autoclave or plastic backed general tape should be used. A length 5cm longer than the width of each end of the tray should be cut. One length should be placed over one end of the tray and stuck 1cm in from the tray edge. -
Page 10: Gel Staining
4. Once loaded, gently immerse the gels within the buffer, stacking them carefully on top of each other. Replace lid and connect unit to the power supply using cables. 5. Typically gels are run at between 90 and 150 volts. However, maximum voltages are indicated on the serial badge of each unit. -
Page 11: Solutions
2. Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley-Interscience,1989. Solutions:- 1x TAE 40 mMtris (pH 7.6), 20 mM acetic acid, 1 mM EDTA. 50x (1L) dissolve in 750 ml distilled water: 242 g tris base (FW = 121) 57.1 ml glacial acetic acid 100 ml 0.5 M EDTA (pH 8.0). -
Page 12: Symbols & Conventions
SYMBOLS AND CONVENTIONS The following chart is an illustrated glossary of the symbols that may be used in this manual or on the product. The electrical warning indicates the presence of a potential hazard which could result in electrical shock. CAUTION This symbol refers you to important operating and maintenance (servicing) instructions within the product Instruction Manual. -
Page 13: Declaration Of Conformity
Number: CE 00601 Labnet International, Inc,., 31 Mayfield Ave., Edison, NJ 08837 USA Labnet International declares that the devices described below are in conformity with the EC directives listed. In the event of unauthorized modification of any of the devices listed below, this declaration becomes invalid. - Page 14 NOTES...
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Page 15: Warranty
This warranty gives you specific legal rights. You may have other rights which vary from state to state. No individual may accept for, or on behalf of Labnet International, Inc., any other obligation of liability, or extend the period of this warranty.
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