Wavelength Accuracy - Jasco V-530 Hardware/Function Manual

Uv/vis spectrophotometer
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4.3.3 Wavelength Accuracy

To check wavelength accuracy, measure the spectra of the D
emission line at 656.1 nm and 486.0 nm.
(1) Light only the Deuterium lamp (see item (1) in Section 4.3.2).
(2) Start [Spectrum Measurement] from the [Spectra Manager] window and set
the following parameters.
<<Parameters for spectrum with an emission line at 656.1 nm>>
V-530 measurement parameters
Photometric Mode Sample
Response
Fast
Scanning Speed
40 nm/min
Start
658 nm
End
654 nm
Data Pitch
0.1 nm
Display
0~100
No. of Cycles
1
Note:
PMT voltage
<<Parameters for spectrum with an emission line 486.0 nm>>
Start
End
All other parameter settings are the same parameters as shown above.
(3) Measure the spectrum of the D
at 486.0 nm with the sample chamber empty. Measurement examples are
shown in Fig. 4.5 and Fig. 4.6.
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V-550/560/570
parameters
Photometric Mode Sample
Response
Band Width
Scanning Speed
Start
End
Data Pitch
Display
PMT Voltage
No. of Cycles
with the instrument. Set properly.
varies
488 nm
484 nm
lamp with an emission line at 656.1 nm and
2
23
lamp with an
2
measurement
Fast
0.5 nm
10 nm/min
658 nm
654 nm
0.025 nm
0~100
300 V
1

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