Wavelength Accuracy - Jasco V-530 Hardware/Function Manual

Uv/vis spectrophotometer
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Figure 4.4 D
(5) The wavelength repeatability is normal if it is within the following criterion.
Criterion: Within ±0.1 nm of the average value of the peaks of the spectrum with
an emission line at 656.1 nm
(6) Display the [Environment] menu again and return to the original settings (i.e. check
the [Deuterium] and [Halogen] boxes).

4.3.3 Wavelength Accuracy

To check wavelength accuracy, measure the spectra of the D
line at 656.1 nm and 486.0 nm.
(1) Light only the Deuterium lamp (see item (1) in Section 4.3.2).
(2) Start [Spectrum Measurement] from the [Spectra Manager] window and set the
following parameters.
<<Parameters for spectrum with an emission line at 656.1 nm>>
V-530 measurement parameters
Photometric Mode Sample
Response
Scanning Speed
Start
End
Data Pitch
Display
No. of Cycles
Note:
PMT voltage
<<Parameters for spectrum with an emission line 486.0 nm>>
Start
End
All other parameter settings are the same parameters as shown above.
lamp spectra with an emission line at 656.1 nm
2
Fast
40 nm/min
658 nm
654 nm
0.1 nm
0~100
1
with the instrument. Set properly.
varies
488 nm
484 nm
V-550/560/570
parameters
Photometric Mode Sample
Response
Band Width
Scanning Speed
Start
End
Data Pitch
Display
PMT Voltage
No. of Cycles
22
lamp with an emission
2
measurement
Fast
0.5 nm
10 nm/min
658 nm
654 nm
0.025 nm
0~100
300 V
1

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