Safety Considerations To ensure operation safety, this instrument must be operated correctly and maintained according to a regular schedule. Carefully read to fully understand all safety precautions in this manual before operating the instrument. Please take a moment to understand what the signal words WARNING!, CAUTION, and Note mean in this manual.
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Warning labels are attached at several locations on this instrument. Do not remove, deface or damage the warning labels. If a warning label peels off the instrument or becomes illegible, contact your local JASCO distributor and state the part number of the label you want to replace.
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Warning for GROUND Warning for FUSE Figure 2 Warning Labels on the V-550/560/570 Back Panel Warning for carrying The weight (in kilograms) of each instrument is as follows: V-530 V-550 V-560 V-570 16.0 30.5 32.0 33.0 When moving the instrument, hold the handgrips at the bottom of the instrument firmly (see Fig.
· A "Declaration of Conformity" in accordance with the above standards has been made and is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060 CREMELLA (LC), Italy. FCC Statement (for USA only) Federal Communications Commission Radio Frequency Interference Statement W A R N I N G : T hi s equi pm ent generat es, uses, and can radi at e radi o f requency energy.
This manual should be easily accessible to the operator at all times during instrument operation. When not using the instrument, keep this manual in a safe place. If this instruction manual becomes lost, order a replacement from your local JASCO distributor. The instruction manual is divided into following volumes: 1) Hardware manual: Describes the principles of operation, specifications, maintenance, troubleshooting of this instrument.
Installation Requirements To ensure operation safety, observe the following conditions: Do not operate the instrument under voltage fluctuations exceeding 10% of the recommended line voltage. Otherwise, the instrument may not function properly. Frequency or spike noise in the power supply should be minimal. Ensure that the instrument is grounded.
Consumable parts should be ordered according to part number from your local JASCO distributor. If a part number is unknown, give your JASCO distributor the model name and serial number of your instrument.
Limited Warranty Products sold by JASCO, unless otherwise specified, are warranted for a period of one year from the date of shipment to be free of defects in materials and workmanship. If any defects in the product are found during this warranty period, JASCO will repair or replace the defective part(s) or product free of charge.
1. Introduction 1. 1 Layout of t h i s M anual T hi s sect i on descri bes t he l ayout and f unct i on of t hi s m anual . T he V -500 f or W i ndow s Ò i nst ruct i on m anual consi st s of 10 sect i ons, i ncl udi ng t hi s one.
T he f ol l ow i ng not at i onal convent i ons are used t hroughout t hi s m anual : General Notation Notation Meaning [Measurement] menu Names of menus, commands, and text boxes are enclosed in square brackets [ ], followed by a description indicating whether [Parameters...] command the function is a menu, command, text box, or other.
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ei ght w avel engt hs can be set and m easured. [ A bs/ % T M et er] program T hi s program reproduces t he anal og spect rophot om et er on t he C R T screen, so you can read t he absorbance (A bs) or t ransm i t t ance (% T ) at an arbi t rary w avel engt h on t he m et er.
2. 1. 3 [ S pect ra M anager] st art up W hen W i ndow s i s st art ed, [ Jasco] -[ S pect ra M anager] al so st art s. (S ee F i g. 2. 2. ) T he [ S pect ra...
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F i gure 2. 2 W i ndow s screen F i gure 2. 3 [ S pect ra M anager] w i ndow I n t he [ S pect ra M anager] w i ndow , m ake sure t he I nst rum ent i n t he [ I nst rum ent s: ] box i s t he spect rophot om et er you are usi ng.
M anager] w i ndow appears. E xi t i ng t he [ S pect ra M anager] program C l i ck [ A ppl i cat i ons] - [ E xi t ] . E xi t i ng W i ndow s E xi t W i ndow s accordi ng t o t he W i ndow s U ser ’...
2. 3 [S pect r a M anager] M enu T he [ S pect ra M anager] i s used t o st art m easurem ent , spect ra anal ysi s and envi ronm ent set t i ng usi ng t he spect rophot om et er, and sel ect i ng, f or st art i ng up and shut t i ng dow n t he spect rophot om et er, as w el l as f or set t i ng t he com m uni cat i on port .
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[ O pt i on. . . ] I nst al l t he opt i onal accessory (R ef er t o t he accessory i nst ruct i on m anual ). [ A bout . . . ] D i spl ays t he versi on i nf orm at i on of t he cont rol dri ver of t he spect rophot om et er.
3. Quantitative Analysis and Spectrum Measurement T hi s sect i on descri bes quant i t at i ve anal ysi s and spect rum m easurem ent . T he param et ers are descri bed onl y bri ef l y i n order t o cl ari f y t he operat i on f l ow . F ol l ow t he procedures out l i ned bel ow i n order t o becom e f am i l i ar w i t h t he operat i on of V -500W .
Proportional expression Linear expression Quadratic expression Third-order expression Segmented line Logistic function Spline function F i gure 3. 2 C al i brat i on curve m odes T he operat or can set w het her t o use st andard m easurem ent dat a (set of concent rat i on/ absorbance) t o cal cul at e t he cal i brat i on curve.
T he m essage U N D E R I N I T I A LI Z A T I O N appears and m easurem ent param et ers are t ransf erred t o t he spect rophot om et er. W hen t ransf er i s f i ni shed, t he program st art s and t he f ol l ow i ng w i ndow appears. F i gure 3.
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C l i ck <N ew > t o open t he f ol l ow i ng di al og box. F i gure 3. 5 [ Q uant i t at i ve M easurem ent P aram et ers] di al og box C hangi ng m easurem ent param et ers procedure T he [ Q uant i t at i ve M easurem ent P aram et ers] di al og box di spl ays t he i nst rum ent def aul t set t i ngs.
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F i gure 3. 6 [ C al i brat e C urve P aram et ers] di al og box C al i brat i on curve param et er set t i ng S et [ C al i b. curve] t o [ P roport i onal ] . U se t he sam e procedure as f or changi ng param et ers I f [ S t andard bl ank] i s know n, i nput t hat val ue t o t he t ext box.
F i gure 3. 7 [ Q uant i t at i ve M easurem ent ] di al og box M easuri ng t he st andard bl ank S el ect t he [ B l ank] opt i on but t on. P l ace t he st andard bl ank i n t he cel l hol der of t he sam pl e cham ber.
[ U se] f i el d changes f rom [ ---] t o [ U se] . T he cursor aut om at i cal l y m oves t o t he next l i ne. N ot e: T he st andard bl ank val ue i s not subt ract ed f rom t he absorbance i n t he st andard dat a di spl ay f i el d. R epeat st eps 2) and 3) as m any t i m es as t he num ber of st andard sam pl es.
I nval i dat i ng M ove t he cursor t o t he i ncorrect dat a l i ne. U nsel ect t he [ E nabl e C al i b. D at a] checkbox, and t hen cl i ck <A ppend>. T he [ U se] changes t o [ ---] .
F i gure 3. 14 [ P ri nt ] di al og box C l i ck <O K > t o pri nt t he quant i t at i ve anal ysi s dat a. 3. 1. 9 E xi t i ng quant i t at i ve anal ysi s C l i ck [ F i l e] - [ E xi t ] t o ret urn t o t he [ S pect ra M anager] w i ndow af t er m easurem ent i s f i ni shed.
3. 2 S pect r um M easurem ent T hi s sect i on descri bes t he procedures f or st art i ng t he S pect rum M easurem ent program , m easuri ng st andard sam pl es, savi ng m easured spect ra t o di sk, and pri nt i ng dat a. 3.
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3. 2. 3 S et t i ng m easurem ent param et ers C l i ck [ M easurem ent ] - [ P aram et er. . . ] . T he f ol l ow i ng di al og box appears. T he di al og box consi st s of t w o pages, [ P aram et er] and [ D at a F i l e] .
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3. 2. 4 S et t i ng t he basel i ne(or M easurem ent ) T he basel i ne def i nes t he “ 0 ” absorbance (100% f or t ransm i t t ance) l evel . T he basel i ne val ue i s subt ract ed f rom t he m easured dat a (di vi ded f or t ransm i t t ance) i n order t o det erm i ne t he correct spect rum of a sam pl e.
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F i gure 3. 19 [ B asel i ne M easurem ent ] (1) di al og box N ot e: I f t he <M easure> but t on i s cl i cked w i t hout put t i ng a check m ark t o [ F ul l W avel engt h] , m easurem ent w i l l st art i m m edi at el y w i t hout di spl ayi ng F i g.
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3. 2. 5 S am pl e m easurem ent P l ace a sam pl e i n t he cel l hol der on t he near-si de of t he sam pl e cham ber, and t hen cl ose t he l i d. Sample Chamber F i gure 3.
3. 2. 6 S pect rum save S pect ra can be saved i n a f i l e. C l i ck [ F i l e] - [ S ave A s. . . ] . T he f ol l ow i ng di al og box appears. F i gure 3.
C l i ck [ F i l e] - [ P ri nt . . . ] t o pri nt t he spect ra. 3. 2. 8 I nst rum ent shut dow n E xi t i ng t he [ S pect ra A nal ysi s] program C l i ck [ F i l e] - [ E xi t ] .
4. [Quantitative Analysis] D oubl e-cl i ck [ Q uant i t at i ve A nal ysi s] i n t he [ S pect ra M anager] w i ndow . T he program st art s and t he f ol l ow i ng w i ndow appears af t er spect rophot om et er i ni t i al i zat i on.
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M anager] . [ M et hod] m enu [ N ew . . . ] C reat es a new cal i brat i on curve. [ O pen. . . ] O pens saved quant i t at i ve anal ysi s m et hod f i l es. [ S ave A s.
O pens a saved [ D at a S heet ] f i l e ([ F i l e] -[ O pen. . . ] ) P ri nt s t he dat a f rom t he act i ve w i ndow set by [ P age S et up. . . ] ([ F i l e] -[ P ri nt . . . ] ) 4.
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F i gure 4. 3 [ I nf orm at i on] di al og box F i gure 4. 4 [ D at a S heet ] w i ndow A n unknow n sam pl e can be m easured f rom t he [ D at a S heet ] w i ndow usi ng t he di spl ayed quant i t at i ve anal ysi s m et hod.
4. 1. 2 [ O pen. . . ] O pens a saved [ D at a S heet ] f i l e. N ot e: I f an unsaved [ D at a S heet ] or [ C al i brat e C urve] i s i n t he w i ndow w hen [ O pen. . . ] i s cl i cked, a m essage appears t o ask t he operat or w het her t he dat a shoul d be saved.
4. 1. 4 [ S ave A s. . . ] S aves t he act i ve [ D at a S heet ] under a new f i l enam e. M easurem ent param et ers and cal i brat i on curve dat a are al so saved.
4. 2 [M et h od] m enu 4. 2. 1 [ N ew . . . ] C reat es a new cal i brat i on curve. C l i ck [ N ew . . . ] t o open t he f ol l ow i ng di al og box. N ot e: I f a cal i brat i on curve has not been saved, a m essage appears t o ask t he operat or w het her t o save i t .
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0. 5 2 (L2) 5 (L5) 10 (L10) [ M et hod] N o. of w avel engt hs used i n quant i t at i ve anal ysi s. S el ect s opt i m um num ber. of w avel engt hs (1, 2, or 3) accordi ng t o t he sam pl e condi t i on. 1-w avel engt h: F or com m on sol ut i on sam pl e.
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F i gure 4. 13 [ C al i brat e C urve P aram et ers] di al og box [ G raph S et t i ng] [ C al i b C urve] T he t ype of cal i brat i on curve. C l i ck t he drop-dow n f orm at box and sel ect a t ype.
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[ E nabl e C al i b. D at a] D at a i n t he st andard dat a di spl ay f i el d can be used f or t he cal i brat i on curve by sel ect i ng t he [ E nabl e C al i b.
<C ancel >: C l oses t he di al og box w i t hout changi ng t he previ ousl y set w avel engt h. <A ut o Z ero> S et s t he absorbance val ue of t he current w avel engt h t o zero. <O K >...
4. 2. 3 [ S ave A s. . . ] S aves quant i t at i ve anal ysi s m et hod dat a, i ncl udi ng cal i brat i on curve, and m easurem ent param et ers. F i gure 4.
4. 2. 5 [ I nf orm at i on. . . ] W hen t he [ M et hod I nf orm at i on] w i ndow i s i n i con f orm , st art i ng t hi s f unct i on opens t he ori gi nal w i ndow agai n.
<C l ose> C l oses t he [ Q uant i t at i ve M easurem ent ] di al og box. <G ot o W L. . . > M oves t he w avel engt h of t he spect rophot om et er t o a set w avel engt h (see F i g.
4. 5. 1 [ F ont . . . ] S et s t he f ont f or t he [ D at a S heet ] or cal i brat i on curve. W hen t he [ C al i brat e C urve] w i ndow i s act i ve, t he f ol l ow i ng w i ndow opens bef ore t he [ F ont ] di al og box appears.
F i gure 4. 27 [ F ont ] di al og box [ F ont ] l i st S el ect a f ont f rom t hi s l i st . [ F ont S t yl e] l i st S el ect a f ont st yl e f rom t hi s l i st .
[ A ut o] checkbox t o set t he scal e t o t he opt i m al val ue accordi ng t o t he cal i brat i on curve dat a. T hi s f unct i on i s act i ve onl y w hen t he [ C al i brat e C urve] w i ndow i s act i ve.
F i gure 4. 32 [ G ri d Li nes] di al og box [ M ai n] S el ect t he [ V ert i cal A xi s] and/ or [ H ori zont al A xi s] checkbox t o di spl ay t he scal e l i ne.
4. 5. 8 [ M arker. . . ] C l i ck [ V i ew ] - [ M arker. . . ] t o set t he t ype, si ze, and col or of t he m arker used t o i ndi cat e speci f i c dat a poi nt s on t he cal i brat i on curve, and w het her t o f i l l t he i nsi de of t he m arker.
5. [Spectrum Measurement] [ S pect rum M easurem ent ] m easures a sam pl e spect rum . I n t he [ S pect ra M anager] w i ndow , doubl e-cl i ck [ S pect rum M easurem ent ] . T he spect rophot om et er i s i ni t i al i zed and t he f ol l ow i ng w i ndow appears. Title Bar Menu Bar Monitor Bar...
[ E xi t ] E xi t s t he spect rum m easurem ent program and ret urns t o [ S pect ra M anager] . [ H el p] m enu [ A bout . . . ] D i spl ays i nf orm at i on such as t he program versi on.
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F i gure 5. 3 [ P aram et ers] di al og box [ P hot om et ri c M ode] S et s t he phot om et ri c m ode. S el ect abl e range: ...
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regi on) N ot e: W hen usi ng t he V -570 f or m easurem ent f rom t he near-i nf rared regi on t o t he vi si bl e regi on i n successi on, t he bandw i dt hs l i st ed i n T abl e 5.
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S canni ng S peed (nm / m i n) ...
<O K > S aves param et ers. <C ancel > R et urns t o t he [ P aram et ers] di al og box w i t hout savi ng t he param et ers. <C ont ent s. . . > D i spl ays t he param et ers sel ect ed f rom t he param et er l i st .
are saved aut om at i cal l y. [ F i l e N am e] T ext box f or i nput t i ng t he f i l enam e of aut om at i cal l y saved dat a. U p t o 5 charact ers m ay be i nput .
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F i gure 5. 8 [ P aram et ers-O pen] di al og box S el ect i ng a f i l enam e f rom [ P aram et ers Li st ] and cl i cki ng <O K > l oads i t i nt o m em ory and rew ri t es [ B asel i ne param et ers] t o t hose of t he f i l e.
F i gure 5. 10 [ B asel i ne M easurem ent ] di al og box C l i ck t he <S t art > but t on. B asel i ne m easurem ent st art s. D uri ng m easurem ent , t he f ol l ow i ng di al og box i s di spl ayed.
F i gure 5. 12 [ G ot o W avel engt h] di al og box [ W avel engt h] T ext box f or i nput t i ng a w avel engt h. <O K > M oves t he spect rophot om et er w avel engt h t o t he set w avel engt h.
6. [Time Course Measurement] M easures changes i n a sam pl e over t i m e at a set w avel engt h. I n t he [ S pect ra M anager] w i ndow , doubl e-cl i ck [ T i m e C ourse M easurem ent ] . T he program st art s, and t he w i ndow show n bel ow opens.
[ H el p] m enu [ A bout . . . ] D i spl ays i nf orm at i on such as program versi on. 6. 1 [M easurem ent ] m enu 6. 1. 1 [ S t art ] S t art s t i m e course m easurem ent .
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F i gure 6. 3 [ P aram et er] di al og box [ P hot om et ri c M ode] S et s t he phot om et ri c m ode. S el ect abl e range: ...
7. [Fixed Wavelength Measurement] [ F i xed W avel engt h M easurem ent ] m easures sam pl e absorbance or t ransm i t t ance at a f i xed w avel engt h. D oubl e-cl i ck [ F i xed W avel engt h M easurem ent ] i n t he [ S pect ra M anager] w i ndow . T he program st art s and t he w i ndow show n bel ow appears.
[ A bout . . . ] D i spl ays i nf orm at i on such as program versi on. 7. 1 [M easurem ent ] m enu 7. 1. 1 [ S t art ] S t art s f i xed-w avel engt h m easurem ent . T o suspend m easurem ent , cl i ck <S t op>. 7.
7. 1. 3 [ P aram et er. . . ] S et s param et ers, and saves t hem t o t he hard di sk. C l i ck [ M easurem ent ] - [ P aram et er. . . ] t o di spl ay t he di al og box show n bel ow . F i gure 7.
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N ot e: W hen usi ng t he V -570 f or m easurem ent f rom t he near-i nf rared regi on t o t he vi si bl e regi on i n successi on, t he bandw i dt hs l i st ed i n T abl e 7. 1 shoul d be pai red. I f t he bandw i dt h i s set t o t he sam e (nearl y equal ) val ue, noi se i n t he near-i nf rared regi on m easurem ent val ue w i l l i ncrease.
<O K > E nds param et er set t i ng. C l i ck t hi s but t on t o t ransf er t he set param et ers t o t he spect rophot om et er. A t t he sam e t i m e, t he di al og box cl oses. <C ancel >...
8. [Abs/%T Meter] [ A bs/ % T M et er] i s a di spl ay of t he anal og spect rophot om et er on t he screen. Y ou can read A bsorbance (A bs) or t ransm i t t ance (% T ) at a set w avel engt h. I n t he [ S pect ra M anager] w i ndow , doubl e-cl i ck [ A bs/ % T M et er] .
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M edi um : M ovi ng average duri ng about 1 sec S l ow : M ovi ng average duri ng about 4 sec [ B and w i dt h] S pect ral bandw i dt h. S el ect abl e range vari es accordi ng t o t he m odel . V -530: F i xed at 2 nm V -550/ 560:...
9. [Environment] S yst em hardw are set t i ng, sel f -di agnosi s, opt i onal accessory set t i ng, and w avel engt h correct i on can be st art ed f rom t hi s m enu. I n t he [ S pect ra M anager] w i ndow , doubl e-cl i ck [ E nvi ronm ent ] .
i s t urned O N . I f bot h l am ps are sel ect ed, t he l i t l am p changes f rom hal ogen t o deut eri um at t he set [ W avel engt h f or Lam p E xchange] w avel engt h.
<R et ry> D oes sel f -di agnosi s agai n. <C l ose> E nds sel f -di agnosi s. 9. 3 [A ccessori e s S et t i n g] S et s t he condi t i ons of t he opt i onal i nt el l i gent accessori es t hat have com m uni cat i on f unct i ons. F or det ai l s, ref er t o t he i nst ruct i on m anual s f or t he accessori es.
T em perat ure C ont rol l er [ C ont rol ] check box W hen checked, t he t em perat ure cont rol l er i s used. [ T em perat ure] S et t em perat ure. [ S t i rrer] W hen checked, t he st i rrer i s used.
10. Appendix 10. 1 S pect r a M anager I n st a l l a t i o n 10. 1. 1 B ef ore i nst al l at i on B ef ore i nst al l i ng t he [ S pect ra M anager] , conf i rm t he f ol l ow i ng: T he com put er and al l peri pheral devi ces are properl y connect ed.
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F i gure 10. 2 [ C ont rol P anel ] D oubl e-cl i ck [ A dd/ R em ove P rogram s] i n t he [ C ont rol P anel ] w i ndow . T he w i ndow bel ow appears. F i gure 10.
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S el ect [ I nst al l / U ni nst al l ] , t hen cl i ck t he <I nst al l (I )> but t on. T he w i ndow bel ow appears. F i gure 10.
C l i ck t he <F i ni sh> but t on. T he w i ndow bel ow appears. F i gure 10. 6 [ S pect ra M anager] f or W i ndow s S et up C l i ck t he <N ext >...
C l i ck t he <N ext > but t on. T he di al og box show n i n F i g. 10. 8 appears. F i gure 10. 8 T arget program di rect ory set t i ng f or [ S pect ra M anager] 10.1.2.3 Setting the program directory I nput t he dri ve and nam e of t he t arget [ S pect ra M anager] program di rect ory.
i ndi cat i ng t hat i nst al l at i on has f i ni shed. C l i ck <O K >. 10. 1. 3 I nst al l i ng Spectra Manager from CD-ROM O pen [ C ont rol P anel ] , sel ect [ A dd/ R em ove P rogram s] t o run program . F i gure 10.
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T he next st ep w i l l be t o set up each of t he vari ous appl i cat i ons. C l i ck <st art > on t he t askbar and proceed t o [ program ] , t hen [ Jasco] , t hen [ S pect ra M anager set up] and cl i ck t o run program .
10. 2 S et t i n g t h e S eri a l P ort (R S -232C ) T he seri al port def aul t val ue i s [ C O M 1] . T he seri al port num ber m ust be changed w hen t he S pect rophot om et er i s cont rol l ed t hrough anot her port .
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JASCO Corporation 2967-5 Ishikawa-machi, Hachioji TOKYO, Japan Printed in Japan...
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