GE PhastSystem User Manual page 52

5. Operation
52
Naming a method
4. Press "name method":
DEV 1 [_
5. Press "step forward" six times to display the name field for
method 7:
DEV 7 [_
6. Press " " or " " until the first character you want in the
name appears in the parentheses to the right, for example
method 7 can be called COOM-IEF (Coomassie for IEF runs):
DEV 7 [_
7. Press "do" to enter this character:
DEV 7 [C_ ]
8. Continue with steps 6 and 7 until all characters are entered:
DEV 7 [COOM-IEF_ ] (F)
To continue programming the method
9. Press "DEV method file", "7", and "do" to select method 7
again:
DEV 7 NAME COOM-IEF
The name of the method will now appear on the display.
Programming the Ct curve
10. Press "step forward" for the Ct curve instruction:
DEV 7 Ct(5,30,40,50)°C = (1.0,1.0,1.0,1.0)
If you plan to use temperature compensation, follow the
instructions on page 61 to program the Ct curve; otherwise, go
directly to step 11 below.
Programming an alarm
11. Press "step forward" for the alarm instruction.
12. As for separation methods, you can program an alarm to sound
at a certain time during the method; first enter the step number
during which the alarm will sound, for example, step 10:
EXTRA ALARM TO SOUND AT 7.10 t = 00.0min
13. Press " " and enter the time when the alarm will sound
during the step, for example after 7.0 minutes in step 10:
EXTRA ALARM TO SOUND AT 7.10 t = 07.0min
Programming method steps
14. Press "step forward" and enter the port number that the first
solution will enter through (enter 1 to 9), for example, enter
port 1:
DEV 7.01 IN = 1 OUT = 0 t = 00.0min T = 00°C
15. Press " " and enter the port number the first solution will exit
through (enter 0 to 9), for example, enter port 1 to recycle
solution 1:
DEV 7.0 IN = 1 OUT = 1 t = 00.0min T = 00°C
] (A)
] (A)
] (C)
(C)