Introduction - GE PhastSystem User Manual

1. Introduction

™
PhastSystem consists of a separation and control unit, a development
unit, high-performance PhastGel
technical support package. These components work together to form
a system for fast, high-resolution, and reproducible electrophoresis.
With PhastSystem, isoelectric focusing is as easy to perform as gel
electrophoresis; Coomassie staining is as easy as silver staining. The
schematic diagram below illustrates the steps involved in producing a
finished electrophoresis gel using PhastSystem with PhastGel
separation media.
Flow diagram for PhastSystem
Separation and control unit
Place 1 or 2 gels on
the separation bed
Load PhastGel
sample applicator(s)
Select a programmed
separation method
and press the start
button
when alarm sounds
remove the gel(s)
Total time 26-50 min.
or
Specific detection via
conventional methods
e.g. zymograms, auto-
radiography, blotting
The time intervals listed above will depend on the technique that is run.
® separation media, accessories, and a
Development unit
Place gel(s) in
3 min. development chamber
Select a programmed
3 min. development method
and press the start button
when method stops
remove the gel(s) and
analyze the results
20-45 min.
1. Introduction
1 min.
30-90 min
Total time 32-92 min.
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