GE PhastSystem User Manual page 48

5. Operation
48
7. Place the PhastGel buffer strip holder over the gels by first sliding
the buffer strip holder forward so that the two black pins and the
holes in the holder form a hinge. Lower the buffer strip holder
onto the separation bed.
Fig. 26. Positioning the buffer strip holder.
8. Take a pack of native or SDS buffer strips from the refrigerator.
Peel back the foil over two buffer strips and remove them with
a spatula.
Note: Gloves should be worn when handling buffer strips, to
prevent eventual disturbance from finger proteins.
9. Insert the buffer strips into the compartments in the buffer strip
holder; one in the anode and one in the cathode compartment.
Repeat this for the second gel. Gently press down on them to
ensure good contact between the buffer strips and the gel.
Buffer strips will protrude above the compartments by about
1-2 mm.
10. Lower the electrode assembly so that the outer electrodes (the
cathode and the anode furthest from it) rest evenly on the
buffer strips.
11. Gently press down along the top of the electrodes: The electrodes
must have complete and even contact with the buffer strips.
12. Lower the sample applicator arm.
Sample application
1. Load the sample applicator(s) as described on page 39.
2. Slide the loaded sample applicator(s) into the slot nearest the
cathode (-).
3. Close the separation compartment lid.