GE PhastSystem User Manual page 56

5. Operation
56
Starting the run
1. Press "DEV start/stop" and enter the programmed method
number:
START DEV METHOD
2. Once you enter the method number, the method name (if you
gave your method a name) will appear in parentheses beside the
method number, for example:
START DEV METHOD
The method always starts at the first step unless you enter a
different step number after the period.
3. Press "do" to confirm.
During the course of the run, you may at any time start a separation
run or program another separation or development method. Press
"DEV real condition" to display the progress of method.
Monitoring the progress
The display will keep you informed about the progress of the method
and the event that is taking place. The sequence of events during a
development step is described below.
First, the development chamber is emptied of eventual residual liquid
through port 0 (P0), to waste. This is a precautionary step (it is not
programmable):
DEV 7.01 t = 0.0 min T = 22°C
Next, the chamber will be filled with liquid through the programmed
in-port, for example through port 1 (P1):
DEV 7.01 t = 0.0 min T = 22°C
It takes approximately 15 seconds to fill the chamber. When the cham-
ber is full, the solution is heated to the programmed temperature and
the gels are rotated in the solution until the end of the step, that is, the
gel is being processed:
DEV 7.01 t = 0.5 min T = 28°C
The in-port tube number (Pl) will remain on the display until the
chamber empties.
The time t, shown on the display, starts at zero and counts up to the
time programmed for the step. The temperature T, is the actual
temperature of the solution.
When the processing time equals the programmed time for the step,
the chamber empties and the out port number (Pl) is shown on the
display, for example:
DEV 7.01 t = 10.5 min T = 50°C
After the chamber is emptied, the in-port tube is cleared of solution by
compressed air. The display will show, for example:
DEV 7.01 t = 0.0 min T = 26°C
0.00 <do>
7.00 (COOM-IEF) <do>
EMPTYING P0
FILLING P1
PROCESSING P1
EMPTYING P1
CLEANING TUBE