Using The Keyboard - GE PhastSystem User Manual

3. Description of the system
3.4 Using the
keyboard
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PhastGel silver kit
Silver staining is traditionally a complex method, with several steps
that are both time and temperature sensitive. With the automated
development in PhastSystem even complex methods have become easy
to manage reproducibly. With PhastGel silver kit all the solutions that
can be critical are conveniently packed in ready-to-use bottles. The
user only has to provide ethanol, acetic acid, trichloroacetic acid,
glycerol, tris-HCl and water, all normally available in most
laboratories.
The aim of this section is to prepare you for programming, editing,
and running separation and development methods. Before you begin
to program or run a method, you should know what happens when
you turn on the system, know what the LEDs are form, and be
familiar with the display and the keyboard. In this section, the key-
board will be described in key-blocks; the numerical pad, the
programming and editing keys, and the run control and monitoring
keys.
In this manual, keys are always referenced by the key text in quota-
tion marks.
Turning on the system
The power on/off button is placed at the back of the separation and
control unit. The microprocessor automatically runs a diagnostic test
every time the unit is turned on. The test includes the temperature
sensors, back-up battery, and level sensor. When the system is turned
on, the display shows:
DIAGNOSTICS IN PROGRESS
After a few seconds the display changes to:
DIAGNOSTICS SUCCESSFULLY COMPLETED
or, if an error is found, an error message appears and an alarm
sounds.
If no error is found, the separation program mode i automatically
selected.
The LEDs
SEP ON and DEV ON are lit when a separation method or a
development method is running. If you pause a run, the corre-
sponding LED will blink as a remember.
The PROGRAM MODE LED is lit when you select one of the keys
"SEP method file" of "DEV method file" for programming and when
the system is turned on.
The REAL CONDITION LED is lit when a method is started or
when you press one of the keys "SEP real condition" or "DEV real
condition".
One or more of the LEDs may be lit at one time since you can be
running both a separation and development method while you are
programming a method.