Operation; Programming Separation Procedures - GE PhastSystem User Manual

5.1 Programming
separation
procedures

5. Operation

The aim of this chapter is to show you how to program a separation
method and a development method, how to load samples into the
sample applicators, and how to run PhastGel lEF media,
electrophoresis titration curves, and PhastGel homogeneous and
gradient media.
Introduction
Nine separation methods are available for programming. Each
separation method you program can contain two sample application
instructions, an extra alarm instruction, and up to nine method steps.
The instructions and steps appear on the display, one at a time. A
cursor rests under the field to be programmed. For example, in
method 1 the first instruction will be:
SAMPLE APPL. DOWN AT 1.0 0000 Vh
At the cursor position, enter the step number for sample application,
for example, during step 2. By pressing " " the cursor will move to
the next field:
SAMPLE APPL. DOWN AT 1.2 0000 Vh
At this cursor position, enter the number of volthours that will elapse
during step 2 before sample application.
By pressing "step forward" twice, the first step of method 1 appears
on the display.
SEP 1.1 0000V 00.0mA 0.0W 00°C 0000Vh
Each method step finishes after a programmed number of volthours.
A method step can have up to 9999 volthours. You must also pro-
gram the following parameters for each step:
• Voltage in volts (V): 1 to 2000 V
• Current in milliamperes (mA): 0.1 to 50.0 mA
• Power in watts (W). 0.1 to 7.0 W
• Separation bed temperature in °C: 0° to 70°C (see cooling
capacity, page 14)
Important! Program methods for one gel.
Methods must always be programmed for one gel, regardless of
whether you plan to run one or two gels. When you start a separation
run, a command will appear on the display where you must enter the
number of gels you plan to run. When you run two gels (which must
be the same type, e.g. two PhastGel IEF 3-9 gels), the current and
power are automatically adjusted so that both gels run under the
same conditions according to the programmed method. The sum of
current for both gels cannot exceed 50.0 mA, and the sum of the
power for both gels cannot exceed 7.0 W. The following three ex-
amples illustrate this:
5. Operation
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