Programming Development Procedures - GE PhastSystem User Manual

5. Operation
5.5 Programming
development
procedures
50
During the course of the run, you may at any time start a development
run for a finished gel or program another separation or development
method. To go back and check on the separation run in progress,
press "SEP real condition".
Stopping the run
1. When the alarm sounds, check to see that the tracking dye has
reached its proper distance from the electrode for SDS-PAGE. If
it hasn't, the method can be allowed to proceed until it does.
The alarm is temporarily stopped by pressing " SEP real
condition".
2. To stop the method, press "SEP start/stop".
PRESS <do> TO END SEP METHOD
3. Press <do> to confirm
The display will show the temperature of the separation bed
and the accumulated volthours for the method just ended:
SEP 0V 0.0mA 0.0W 18°C 300 AVh
Proceed with development immediately after the method is
stopped.
This section describes the procedures for programming and running
development methods. First, an introduction to programming is
given, including a section on temperature control. Then, a step by
step instruction for programming development methods is given,
followed by the procedure for running the methods. Finally,
temperature compensation is described.
PhastGel media can also be electrotransferred to an immobilizing
membrane with the help of PhastTransfer. This is a rapid, economical
and efficient method of blotting. For more detailed information,
please contact your GE Healthcare representative.
Introduction
Programming development methods is similar to programming
separation methods. The only differences are the parameters to be
programmed.
You may program and save up to nine development methods. Each
method contains 20 steps available for programming. For each step,
the following parameters are programmed:
• The IN-port; the port the solution will enter through: Ports 1 to
9 can be used; port 0 is reserved for waste.
• The OUT -port; the port the solution will exit through: Ports 0
to 9 can be used.
• The duration of the step, t, in minutes. Each step can be up to
99.9 minutes.
• The actual temperature, T, the step will be processed at; the
maximum temperature is 50°C. The development chamber can
only heat solutions.