Phastgel Media And Chemicals - GE PhastSystem User Manual

3. Description of the system
3.3 PhastGel media
and chemicals
18
Table 1: A general guide for the chemical resistance of the wetted parts in the
development unit.
1
Wetted parts Material of
construction
Distributor and PVDF
distributing plate PVDF
Gasket (10-port fluoro rubber
valve)
Tubin (10-port Teflon
to chamber)
Tubing (bottles PVC
to 10-port valve)
Chamber, gel stainless
holder, and temp. steel
sensor
Chamber lid gasket EPDM
Chamber lid PP 5
1 These parts are illustrated on pages 16, 17 and 79.
2
Polyvinylidine fluoride
3 Polyvinyl chloride
At present, PhastGel media are available for four types of electrco-
phoretic techniques; native polyacrylamide gel electrophoresis
(PAGE) in gradient or homogeneous gels, SDS-PAGE in gradient or
homogeneous gels, and isoelectric focusing (IEF). These gels can be
combined for twodimensional techniques.
PhastGel media are made of polyacrylamide bonded to a transparent
polyester backing. The gel surface is covered with a plastic film which
prevents drying and contamination. This film must he peeled off
directly before use, that is, after the gel has been positioned onto the
separation bed, and excess water has been removed.
PhastGel media are individually packaged in airtight envelopes. Once
removed from their package, gels should be used immediately.
PhastGel chemicals include buffer strips for electrophoresis and a
Coomassie-type stain, PhastGel Blue R. PhastGel media and chemi-
Generally
resistant to
2 strong acids and
2
bases in moderate acids
concentration and
alcohols and
hydrocarbons
moderate acids,
strong bases,
many solvents,
alcohols,
aldehydes
most chemicals
3
strong acids and
bases in moderate hydrocarbons
concentration,
alcohols,
aldehydes, and
bleach
most chemicals
4
strong acids and
bases, alcohols,
aldehydes, and
ketones
strong acids in
moderate con-
centration in high
concentration,
alcohols, alde-
hydes, and
ketones
4 Ethylene propylene copolymer and terpolymer
5
Polypropylene (or polypropene)
Generally
attacked by
ketones, esters, and hot
hot strong acids, esters,
ketones, and bleach
extreme conditions
hot acids, ketones, and
long exposure to salt
solutions
hot acids and aromatic
hydrocarbons
hot strong acids, aromatic
hydrocarbons, and bleach