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Problem
2. Section compression
The sections are very
compressed, show folds or are
squeezed together.
3. "Stripes" in sections
For knife holder E
4. Noises during sectioning
The knife "sings" when
sectioning hard specimens.
The sections show scratches
or chatter marks.
8.2
Instrument malfunctions
Problem
1. There is no further feed
motion and thus no sectioning.
2. High blade consumption
Leica RM2125 RTS
Possible cause
• The blade is dull.
• The pressure plate is damaged
or incorrectly adjusted.
• Clearance angle of the knife/
blade too small.
• The blade is dull.
• The specimen is too warm.
• The sectioning speed is too
fast.
• There is an accumulation of
paraffin on the rear pressure
plate of the knife holder.
• The sectioning speed is too
fast.
• The clearance angle is too
wide.
• Insufficient clamping of
specimen and/or knife holder.
Possible cause
• The front end position has been
reached.
• Too great of a sectioning force
was applied.
Troubleshooting
Corrective action
• Laterally displace the knife
holder or insert a new blade.
• Insert a new pressure plate or
use a new knife holder.
• Methodically experiment with
larger clearance angle settings
until you have found the
optimum angle.
• Use another area of the blade
or a new blade.
• Cool the specimen before
sectioning.
• Reduce the sectioning speed.
• Remove paraffin from this area
on a regular basis.
• Turn the handwheel at a slower
speed.
• Methodically reduce the
clearance angle setting until
you have found the optimum
angle.
• Check all screw and clamp
connections on the specimen
holder system and the knife
holder. If necessary, tighten the
levers and screws.
Corrective action
• Run the specimen back by
turning the coarse driving
wheel.
• Adjust the sectioning speed
and/or section thickness when
trimming. Select a smaller
section thickness, rotate the
handwheel more slowly.
8
63
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