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Standard Operating Procedure
Title:
Use and maintenance of Leica RM2125 RTS rotary microtome.
Location:
H27
1. PURPOSE
To describe the use and maintenance of the Leica RM2125 RTS rotary microtome that is located in the
CL2 CBE laboratory H27.
2. SCOPE
This SOP applies to CBE personnel using the Leica RM2125 RTS rotary microtome for the sectioning
of fixed soft tissues that have been embedded in wax or other hard polymers. The microtome is
assigned to H27 which is used for histological processing and this system should not be transferred to
another lab. Only authorised and trained personnel may use the microtome.
3. RESPONSIBILITES
3.1. Authorised laboratory staff shall:
(i) Work in accordance with the instructions for operation and maintenance detailed in this SOP,
Risk Assessment and the operator manual.
(ii) Carry out the routine inspection, cleaning, and maintenance of the equipment, as required.
(iii) Record any adverse events and alarms that indicate non-conformance or malfunction on the
Maintenance Record and notify the Laboratory Manager/Responsible Person.
3.2. The Responsible Person/Laboratory Manager shall:
(i) Ensure that authorised laboratory personnel are given suitable information, instruction, training
and supervision in the correct use and maintenance of the equipment.
(ii) Investigate any reported problems, adverse event, alarms, or non-conformities associated with
equipment usage.
(iii) Organise the maintenance, repair, or servicing of the equipment by trained and authorised
contract / service personnel.
4. EQUIPMENT AND MATERIALS
1. Leica RM2125 RTS rotary microtome and accessories
2. Blades
3. Brush
4. Appropriate PPE (labcoat, gloves, overshoes and eye protection, where appropriate)
Version 001
Written by: Sotiria Toumpaniari
CONTROLLED COPY
Centre for Biological Engineering
Effective Date: 24.05.2021
Reviewed by:
Page 1 of 11
Review 24.05.2023
Approved by: C.Kavanagh
SOP194
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Summary of Contents for Leica RM2125 RTS
- Page 1 Use and maintenance of Leica RM2125 RTS rotary microtome. Location: 1. PURPOSE To describe the use and maintenance of the Leica RM2125 RTS rotary microtome that is located in the CL2 CBE laboratory H27. 2. SCOPE This SOP applies to CBE personnel using the Leica RM2125 RTS rotary microtome for the sectioning of fixed soft tissues that have been embedded in wax or other hard polymers.
- Page 2 5. PROCEDURE 5.1. Description of the system The Leica RM2125 RTS is a manually operated rotary microtome for creating thin sections of specimens of varying hardness for use in routine and research laboratories in the fields of biology, medicine, and industry. It is designed for sectioning soft paraffin specimens as well as harder specimens, as long as they are suitable for being cut manually.
- Page 3 Centre for Biological Engineering Standard Operating Procedure SOP194 Title: Use and maintenance of Leica RM2125 RTS rotary microtome. Location: 5.3. Operation 5.3.1. Clamping specimen on universal cassette clamp 1. Always clamp the specimen block BEFORE clamping the knife or the blade.
- Page 4 Standard Operating Procedure SOP194 Title: Use and maintenance of Leica RM2125 RTS rotary microtome. Location: 5.3.3. Adjusting the clearance angle 1. The index marks (0°, 5° and 10°) for adjustment of the clearance angle (10) are located on the right side of the knife holder (8).
- Page 5 Centre for Biological Engineering Standard Operating Procedure SOP194 Title: Use and maintenance of Leica RM2125 RTS rotary microtome. Location: Note: The user can switch the specimen retraction ON or OFF. (ii) To do so, use an Allen key with handle, No. 4 (included in the delivery package) and turn the screw depicted in the detail picture (Fig.
- Page 6 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP194 Title: Use and maintenance of Leica RM2125 RTS rotary microtome. Location: 5.3.6. Cutting (trimming) the specimen 5.3.6.1. Coarse feed wheel (15) The coarse feed serves for a fast horizontal forwards movement of the specimen – towards the knife –...
- Page 7 2. Trim the specimen by rotating the handwheel (15) until the desired specimen plane is reached. 5.3.6.4. Trimming with the mechanical trimming function The Leica RM2125 RTS is equipped with a mechanical trimming function that is activated via the trimming lever (20). The trimming lever has 3 click stops: 0 μm, 10 μm, and 50 μm.
- Page 8 Centre for Biological Engineering Standard Operating Procedure SOP194 Title: Use and maintenance of Leica RM2125 RTS rotary microtome. Location: CAUTION: The handwheel continues turning if it has been rotated very quickly and then released – this can cause crushing or other injuries! 8.
- Page 9 Centre for Biological Engineering Standard Operating Procedure SOP194 Title: Use and maintenance of Leica RM2125 RTS rotary microtome. Location: Knife holder E Take the knife holder apart for cleaning. The front pressure plate can be removed for cleaning. To do so, proceed as follows: Fold knife guard (5) downward.
- Page 10 Have the instrument checked at least once a year by a qualified service technician authorized by Leica. At the end of the warranty period, enter into a service contract. For more information, please contact your local Leica technical service center.
- Page 11 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP194 Title: Use and maintenance of Leica RM2125 RTS rotary microtome. Location: 6. DOCUMENTATION Equipment Maintenance Log Microtome Training Agreement This form can be found on the Loughborough University LEARN module for the Centre of Biological Engineering under QS-Form templates.
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