Leica RM 2235 User Manual
Rotary microtome
Hide thumbs
Also See for RM 2235:
- Instructions for use manual (78 pages) ,
- Instruction manual (50 pages)
Related Manuals for Leica RM 2235
Summary of Contents for Leica RM 2235
- Page 1 Rotary Microtome Instruction Manual Leica RM2235 V1.4; Rev B, English – 02/2010 Always keep this manual with the instrument. Read carefully before working with the instrument.
- Page 3 Leica reserves the right to change technical stand it following thorough investigation in this specifications as well as manufacturing pro- field.
-
Page 4: Table Of Contents
Table of contents Important notes ........................... 6 Safety ............................7 Safety notes ..........................7 Warnings ............................7 Integrated safety devices ......................10 Instrument components and specifications ............... 12 Overview – instrument components ..................12 Instrument specifications ......................13 Technical data ........................... 14 Startup ............................ - Page 5 6.10 Ordering information ......................... 49 Customized solutions ......................51 Possible faults ..........................51 Instrument malfunctions ......................51 Cleaning and maintenance ....................52 Cleaning the instrument ......................52 Maintenance instructions ......................54 Lubricating the instrument ....................... 55 Warranty and service ......................56 Leica RM2235...
-
Page 6: Important Notes
1. Important notes Symbols in the text and their meanings Intended use of instrument The Leica RM2235 is a manually operated rota- Dangers, warnings and cautions ap- tion microtome for creating thin sections of pear in a gray box and are marked by... -
Page 7: Safety
The protective devices on both instrument and accessories may neither be removed nor modi- fied. Only service personnel qualified by Leica may repair the instrument and access the in- strument’s internal components. 2.2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. - Page 8 2. Safety Warnings – Safety instructions / warning labels attached to the instrument • Safety instruction labels on the instrument marked with a warning triangle indicate that the correct operating instructions (as described in this manual) must be followed when operating or replacing the instrument component bearing the label.
- Page 9 • Do not use any solvents containing acetone or xylene for cleaning! • Ensure that no liquids enter the interior of the instrument when cleaning! • When using detergents please comply with the safety precautions of the manufacturer. Leica RM2235...
-
Page 10: Integrated Safety Devices
2. Safety 2.3 Integrated safety devices Locking the handwheel There are two ways of locking the handwheel (12): Using the lever (3) on the right side of the micro- tome base plate, the handwheel can be braked in almost any position. •... - Page 11 Knife holder E-TC tions of the knife holder can occur. Clamping lever for the blade (10) at the right, clamping lever for the later- al displacement (11) at the left. Fig. 4 Leica RM2235...
-
Page 12: Instrument Components And Specifications
Instrument components and specifications 3.1 Overview – instrument components Tray Handwheel Universal locking cassette clamp mechanism Smooth- Knife holder base, turning without lateral handwheel movement feature Lever for Removable section activating the waste tray handwheel brake Clamping lever of the knife holder base Window for Directional... -
Page 13: Instrument Specifications
3. Instrument components and specifications 3.2 Instrument specifications Basic instrument with mechanical trim function, lateral coarse drive The Leica RM2235 rotary microtome is equipped with a low-maintenance,- slack-free micrometer drive, with vertical and horizontal specimen feed realized via low-maintenance cross roller bearings. -
Page 14: Technical Data
Instrument components and specifications 3.3 Technical data General Approvals: The instrument-specific marks are located on the rear panel of the instrument next to the name plate. Operating temperature range: +10 °C to +35 °C Temperature range during storage: +5 °C to +55 °C Relative humidity: max. -
Page 15: Startup
4. Startup 4.1 Standard delivery The Leica RM2235 standard delivery includes: 1 Leica RM2235 basic instrument 1 handwheel, complete ..............14 0500 38181 1 section waste tray ............... 14 0502 37931 1 tool set – consisting of: .............. 14 0500 38600 1 Allen key with handle, size 5 .......... -
Page 16: Unpacking And Installation
4. Startup 4.3 Unpacking and installation When the instrument is delivered, check the tilt indicators on the packaging. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage. - Page 17 • To move the instrument, hold it by the front of the base plate, lift it up gently and slide it on its slides. Observe the correct resting angle to the table to avoid pinching your fingers. Fig. 8 Leica RM2235...
-
Page 18: Assembling The Handwheel
Startup 4.4 Assembling the handwheel The feather key (4) is loosely The handwheel has to be assembled before attempting placed in the handwheel shaft (1) to use the instrument. and fixed in place with a tie-rap The necessary parts and tools can be found in the tool- during transport. -
Page 19: Inserting The Universal Cassette Clamp
(61) clockwise as far as it will go. Fig. 10 Since all stage clamps available as accessories are equipped with the same kind of guide on the back, they are inserted in the same way described here using the example of the cassette clamp. Leica RM2235... -
Page 20: Inserting The Knife Holder
Startup 4.6 Inserting the knife holder Setting up the knife holder base • Release the clamping lever (50) by rotating it counterclockwise. • Insert the knife holder base (51) with the notch (52) on the bottom into the T-piece (55) of the microtome base plate (53). -
Page 21: Operation
The coarse driving wheel also turns during sectioning. Therefore it must not block whilst the handwheel is be- ing turned during sectioning; other- wise, no feed motion can take place and thus also no sectioning. Fig. 14 Leica RM2235... -
Page 22: Mechanical Trimming Function
5. Operation 5.1.3 Specimen retraction Magnet for The specimen retraction serves for protecting holding the the knife and the specimen. When the retrac- wrench size 4 tion is switched on, the object is drawn back 200 µm into the upper end position after the sectioning stroke during the return movement. -
Page 23: Specimen Holder With Precision Orientation
The usable angle is only about 4° in is noticeable during turning. this case. • To lock the current orientation, turn the eccentric lever (29) backwards. Leica RM2235... -
Page 24: Adjusting The Clearance Angle
5. Operation 5.2 Adjusting the clearance angle • The index marks (0°, 5° and 10°) for adjust- ment of the clearance angle (59.1) are locat- ed on the right side of the knife holder (57). • There is also an index mark (59.2) on the right side of the knife holder basis (51) which serves as a reference point when adjusting the clearance angle. -
Page 25: Fine Adjustment Of The Force Balance
• If the object head moves downwards, turn the screw approx. turn clockwise. • If the object head moves upwards, turn the screw (34) approx. turn counterclock- wise. • Continue this procedure until the object head no longer moves once released. Fig. 20 Leica RM2235... -
Page 26: Clamping The Specimen
5. Operation 5.4 Clamping the specimen Always clamp the specimen block BEFORE clamping the knife. Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or specimen, prior to changing the specimen block and during all work breaks! •... -
Page 27: Sectioning
Take care not to block the coarse driving wheel when turning the handwheel! Otherwise there will be no feed motion of the section thickness and thus no sectioning will occur. • Pick up the sections and mount them on microscope slides. Leica RM2235... -
Page 28: Changing The Specimen Or Interrupting Sectioning
5. Operation 5.7 Changing the specimen or interrupting sectioning Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or object head, as well as prior to changing the specimen block and during all work breaks! •... -
Page 29: Optional Accessories
(5). Completely screw • Finally, place the dovetail holder (2) and fasten in the thrust piece with a flat-tip screwdriver. by screwing in the 4 screws (1) using an Allen • Completely screw in the setscrews (3+4). key size 3. Leica RM2235... -
Page 30: Fine-Directional Fixture For Specimen Clamps
Optional accessories 6.1.3 Fine-directional fixture for specimen clamps • Before the fine-directional fixture for speci- men clamps can be mounted, loosen 4 screws (10) (Allen key size 3) and carefully remove the fixture for specimen clamps from the baseplate (9). •... -
Page 31: Quick Clamping System
It is used as specimen holder for use with the fine-directional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps. • Screw the 4 screws (13) into bore A with an Allen key size 2.5 and tighten them. Fig. 22 Leica RM2235... -
Page 32: Specimen Clamps And Holders
Optional accessories 6.2 Specimen clamps and holders All specimen clamps available as accessories can be integrated into either the directional or non-directional specimen holder fixture. 6.2.1 Standard specimen clamp The standard specimen clamps are available in two sizes: 40 x 40 mm and 50 x 55 mm. They are designed for direct clamping of rect- angular blocks. -
Page 33: Foil Clamp Type 1
(74) by turning the set screw (77) against the fixed jaw (73). • Insert the foil clamp in the standard speci- men clamp as shown. • Turn the knurled screw (66) clockwise to clamp the foil clamp in the standard speci- men clamp. Fig. 28 Leica RM2235... -
Page 34: Foil Clamp Type 2
Optional accessories 6.2.4 Foil clamp type 2 The foil clamp type 2 is appropriate for large foil ribbons. It is mounted in the standard specimen clamp. • To open the jaws (78) and (79) lightly loosen the 3 screws ( ) with an Allen key size 4 •... -
Page 35: Universal Cassette Clamp
Universal cassette clamp, ice-cooled Use the ice-cooled UCC with the non- directional specimen holder fixture only! • Ice cubes can be made using the included Paraflex mold (a). • Quick clamping system (b), see page 31. Fig. 31 Leica RM2235... -
Page 36: Super Mega-Cassette Clamp
Optional accessories 6.2.6 Super mega-cassette clamp Fig. 32 Assembly of the super mega-cassette clamp The super mega-cassette clamp should preferably be used with the rigid fixture for specimen clamps. To do so, proceed as follows: Remove the rubber ring (5) only after having attached the cassette clamp on the object head! •... -
Page 37: Holder For Round Specimens
You can now rotate the specimen so that the side you want faces upwards. • To lock it in the position you have chosen, tighten the pin (92) by turning it clockwise. Fig. 33 Leica RM2235... -
Page 38: Knife Holder Base And Knife Holder
Optional accessories 6.3 Knife holder base and knife holder The plastic handles of all clamping levers on the instrument and knife holders can be turned to the position that is most convenient for each user. Pull the grip (94) out of the lever, hold it in this position, and rotate it to the desired position. -
Page 39: Knife Holder E/E-Tc
Prior to inserting the blade, both knife The knife holder E-TC is designed for holder and knife holder base must the Leica TC-65 tungsten carbide have been installed on the instrument! blades. Inserting the blades, knife holder E and E-TC •... - Page 40 Leica Biosystems offers special prices for new clamp mounts in case of damages to the clamp mount after the warranty has expired. In this way, perfect function of the device can be ensured over the course of many years.
- Page 41 The vessel is filled with water up to the blade. After trimming, remove the section waste from the tray and create the sections to be prepared. The sections floating on the surface of the water can be removed using the glass slide. Fig. 40 Leica RM2235...
-
Page 42: Knife Holder N/Nz
Optional accessories 6.3.3 Knife holder N/NZ The knife holders N and NZ are appropriate for standard steel and tungsten carbide knives, profile c and d, up to 16 cm long. The integrated height adjustment feature allows you to also use knives that have been resharpened numerous times. Mounting the knife support bar •... - Page 43 • Push the knife (47) to the left or right as required. • To clamp the knife (47), always tighten the clamping screw (49) first which is located on the side to which the knife has been repositioned by turning it clockwise. Leica RM2235...
-
Page 44: Section Waste Tray
Optional accessories 6.4 Section waste tray • Push the section waste tray (18) from the front to the microtome base plate (53) until it is held in place by the two magnets (39) (on the front of the microtome base plate). To remove the section waste tray, lift it slightly and pull it off towards the back. -
Page 45: Tray
(but for at least six hours) at approx. -23 °C. • Reassemble the freezer plate and insulation jacket and place them on the bench or the instrument. Fig. 45 Leica RM2235... -
Page 46: Universal Microscope Carrier
Optional accessories 6.8 Universal microscope carrier Unpack all accessories in the package and check for completeness. 1 - Baseplate with drilling (a) 2 - Vertical column with size 8 cap screw (b) and lock washer (c) Fig. 46 3 - Horizontal arm with cross-member (d) and support ring (e) 4 - Support plate, large (for RM2235, RM2245 and RM2255) - Page 47 • Slide the horizontal arm (3), flat side facing the lock screw (g), into the cross-member (d) and tighten. For more information on connecting and using the microscope, magnifying lens or cold-light source, please see the appropriate manual. Fig. 51 Leica RM2235...
-
Page 48: Magnifying Lens
Optional accessories 6.9 Magnifying lens The magnifying lens provides a 2x magnification and can be used with all Leica 2200 series rotary micro- tomes. • Open the screw (3) on the horizontal arm of the microscope carrier in a counterclock- wise direction. -
Page 49: Ordering Information
Clamping ring for round specimen holder, d = 6 mm ............ 14 0356 08322 Clamping ring for round specimen holder, d = 15 mm ..........14 0356 09200 Clamping ring for round specimen holder, d = 25 mm ..........14 0356 08320 Leica RM2235... - Page 50 Optional accessories Universal microscope carrier ................... 14 0502 40580 Magnifying lens assembly ....................14 0502 42790 Cold light sources Leica CLS 100X, 100-120V/50-60Hz ................14 0502 30214 Leica CLS 100X, 230V/50-60Hz ................14 0502 30215 Leica CLS 100X, 240V/50-60Hz ................14 0502 30216 Fiber-optic light guide ....................
-
Page 51: Customized Solutions
2. High blade consumption • Too great of a sectioning force was • Adjust the sectioning speed and/or section thickness when trimming. applied. Select a smaller section thickness, rotate the handwheel more slowly. Leica RM2235... -
Page 52: Cleaning And Maintenance
Cleaning and maintenance 8.1 Cleaning the instrument Always remove the knife / blade before detaching the knife holder from the instrument. Always put the knives back into the knife case when not in use! Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife! When using cleaners, comply with the safety instructions of the manufacturer and the labor-safety regulations of your laboratory! - Page 53 (see also Chapter 8.3). • When installing the pressure plate (83) make sure that the upper edge is parallel and level with the rear edge of the knife holder base plate (86). Fig. 56 Leica RM2235...
-
Page 54: Maintenance Instructions
The instrument is basically maintenance-free. To ensure trouble-free operation of the instrument over a long period of time, the following is recommended by Leica: 1. Thoroughly clean the instrument on a daily 3. Have the instrument inspected on a regular basis. -
Page 55: Lubricating The Instrument
• Knurled nuts (73) for measuring height adjustment. Universal cassette clamp: • Shaft (90) and flat coil spring (91) of the clamping lever of the cassette clamp. After lubrication, move the lever back and forth several times. Fig. 58 Leica RM2235... -
Page 56: Warranty And Service
Leica Biosystems Nussloch GmbH guarantees that the contractual prod- uct delivered has been subjected to a comprehensive quality control pro- cedure based on the Leica in-house testing standards, and that the prod- uct is faultless and complies with all technical specifications and/or agreed characteristics warranted.
Need help?
Do you have a question about the RM 2235 and is the answer not in the manual?
Questions and answers