Troubleshooting - Leica LED1 Instructions For Use Manual

10. Troubleshooting

10. Troubleshooting
No routine maintenance is required. There are no user-replaceable components or sub-assemblies
in the light source. Opening the light engine enclosure will void the manufacturer's warranty. If prob-
lems are encountered during installation or routine operation, review the troubleshooting procedures
described below. If the problem remains unresolved, please contact your local Leica sales represen-
tative for assistance.
Troubleshooting Procedures
Problem
No response to TTL trigger
commands
No response to TTL trigger
commands (LED1/3)
No light output in response
to source ON command (se-
rial or TTL). (LED5/LED8 only)
Unusually weak
cence signals across all de-
tection channels
Unusually weak
cence signals in a single de-
tection channel (e.g. DAPI)
High, spatially uniform fluo-
rescence background
Green power indicator does
not light up when the DC
power supply is connect-
ed and the master power
switch (rear panel) is in the
ON position. (LED3 only)
32
Check the following
Check that TTL trigger inputs are polarity positive, and that serial
ON/OFF controls (PC or Control POD) are set to OFF state.
The light output must be turned ON by either the manual light output
switch (LED1 and LED3) or by the Leica POD (LED1) BEFORE applying
the TTL control signal to the shutter.
There are 3 interlocks that need to be closed, check all of these:
• Liquid light guide must be inserted in light output receptacle.
• External gate jumper must be inserted in front panel
• Control key must be inserted in front panel and turned to "on"
position
fluores- Weak fluorescence in all detection channels (DAPI, FITC, TRITC,
Cy5 etc) is likely to be due to poor light transmission by the liquid
light guide, the collimating adaptor or another distal component of
the microscope optical path and not to abnormally low light output
from the light source.
fluores- • Check that the dichroic beamsplitter and emission bandpass
filter in the microscope are compatible with light engine exci-
tation filter specifications shown on the certificate of confor-
mance.
Check that the dichroic beamsplitter and emission bandpass filter
in the microscope are compatible with light engine excitation filter
specifications shown on the certificate of conformance (Figure 1).
Check that the liquid light guide is fully inserted in the front panel
receptacle and is secured by the set screw.