NEW BRUNSWICK SCIENTIFIC BioFlo 415 Manual To Operations page 66

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6. Press the
7. The system will automatically open and close the valves on the unit to
ensure that the system is sterilized properly.
NOTE:
The air inlet and exhaust filter are connected to a solenoid valve, by
means of a cable that should be connected to the controller. This valve
pulses during sterilization mode to ensure sterilization of the inlet and
exhaust filter.
NOTE:
The sample valve should be sterilized separately in an autoclave, then
aseptically connected to the unit in a clean environment.
8. When the sterilization hold phase ("Steril. Time") ends, the operator should
reopen the needle valve on the water return line from the exhaust line.
WARNING!
All metal surfaces remain very hot after sterilization. Be sure to wear
protective gloves when you move the sparge valve from
sterilization to sparge position.
BioFlo 415 M1360-0050
Figure 22: Sterilization Screen
Start
button to initiate the sterilization sequence.
User's Guide