NEW BRUNSWICK SCIENTIFIC BioFlo 415 Manual To Operations page 126

114
Problem
AGITATION:
Agitator does not turn, or turns
only slowly.
DO and pH PROBES:
DO probe readings are erratic.
pH probe readings are erratic.
Probe does not hold calibration.
GASFLOW:
There is insufficient gas flow.
Gas is entering vessel but
display is not reading gasflow.
STERILIZATION:
Unit does not reach sterilization
temperature
GENERAL:
Touchscreen is not responding.
Pressure builds in vessel and
flow goes to Zero.
BioFlo 415 M1360-0050
Possible Solution
•
Make sure the motor is plugged into the cabinet
receptacle; TURN OFF SUMMARY POWER BEFORE
CONNECTING THE PLUG.
•
Recalibrate the probe, carefully following instructions
in this manual.
•
Recharge the probe, carefully following instructions in
this manual.
•
Probe may need a new membrane and a refill of
electrolyte.
•
Check for a secure connection.
•
Replace probe cable or DO probe.
•
Recalibrate the probe, carefully following instructions
in this manual.
•
Check for a secure connection.
•
Gel-filled probe may need replacement.
•
Liquid-filled probe may need a refill of electrolyte.
•
Probe cable may need replacement.
•
Probe may be defective; replace it.
•
pH/DO amplifier on superboard may be defective; call
for service.
•
Inlet or exhaust sterile air filter may be wet or clogged;
replace filter.
•
Check that the air pressure is within the specified
range.
•
Make sure the control mode for DO and for pH is set
to AUTO (not OFF).
•
Make sure that the GasFlow loop is ON.
•
Make sure that the Air loop is in O2 Enrichment mode.
•
Make sure that the DO cascades are Enabled.
•
Mass Flow Controller has been penetrated by
moisture or particulate matter. Contact NBS for
repair/replacement.
•
Water pressure is too high (>10 PSIG), causing relief
valve to open on jacket. Lower the pressure and
restart sterilization cycle.
•
Calibrate touchscreen.
•
Exhaust filter is blocked. Replace filter and verify that
exhaust heater is on.
User's Guide