NEW BRUNSWICK SCIENTIFIC BioFlo 415 Manual To Operations page 115

SAMPLER NEEDLE
After sterilization of the sampler assembly:
1. Temporarily stop the flow of any gas into the vessel, to avoid backpressure against
the harvest/sample port septum.
2. Carefully remove the clamp from the harvest/sample port plug without dislodging
the flange cap.
3. Unscrew the entire cap assembly from the port to expose the port assembly and its
septum in the center.
4. Place a few drops of EtOH or MeOH on top of the septum, then flame it.
5. Reinstall the port cap assembly, with care not to dislodge the flange cap on top.
6. Aseptically unwrap the sampler assembly.
7. Aseptically remove the sampler needle cap, making sure the gasket remains in the
cap.
8. Holding the stem at its top, remove the harvest/sample port flange cap, making
sure the gasket remains in the groove on the port fitting.
9. Aseptically insert the sampler needle into the the harvest/sample septum, until the
sampler and port flanges are flush against each other.
10. Orient the sampler as desired; close the tri-clamp over the connection to secure it.
11. Aseptically connect a sterile sample vial, bottle or other container.
To remove samples from the vessel:
1. Open the sampler valve.
2. Turn on gas flow into the vessel to create some backpressure.
3. Allow the desired amount of product to flow into the sample container.
4. Observe aseptic techniques to remove the container and to cap it.
5. Immediately replace the container with another sterile receptacle.
New Brunswick Scientific
Figure 62: Sampler Assembly
SAMPLER CAP
103
SAMPLER VALVE
Attach a sample
bottle here.
TRI-CLAMPS
User's Guide