Install Liquid Addition Systems - NEW BRUNSWICK SCIENTIFIC BioFlo 415 Manual To Operations

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6. Lift the tubing guide up to the right, and thread the tubing around the pump head,
anchoring the tubing with one finger.
7. Pulling the loop taut, open the lower spring-loaded clip, load the lower clip
channel.
WARNING!
Be careful not to pinch your fingers in the pump head levers.
8. Let the tubing guide drop down to hold the tubing in place, and close the spring-
loaded lever. Make sure it snaps shut.
9. Press and hold the pump mode
100% setpoint and ensure that the pump operates smoothly.
See Section 10.2 for details on pump assignment and Section 13 for details on pump
set-up and operation.
16.2

Install Liquid Addition Systems

Figure 61 (on the following page) is a simplified depiction of a typical addition
system. Depending on the liquids (base, acid, nutrients, media) to be added, your
system may be slightly different.
1. Aseptically install (if applicable) a sterile (0.2µm) filter in one of the two
penetrations on the addition bottle cap.
2. Aseptically connect the tubing, securing it with a plastic tie, to the harvest tube in
the addition bottle. Clamp it off at the top.
3. If you have not already done so, thread the tubing through the selected feed pump.
4. Connect the tubing, securing it with a plastic tie, to the appropriate addition port on
the headplate.
5. Remove the clamp.
CAUTION!
Proper pH control is critically dependent on tubing size, which should be
as small as possible. Consult Table 9 for guidance.
BioFlo 415 M1360-0050
Prime
button or change the pump mode to
ON
at
User's Guide