NEW BRUNSWICK SCIENTIFIC BioFlo 415 Manuals

Manuals and User Guides for NEW BRUNSWICK SCIENTIFIC BioFlo 415. We have 1 NEW BRUNSWICK SCIENTIFIC BioFlo 415 manual available for free PDF download: Manual To Operations

NEW BRUNSWICK SCIENTIFIC BioFlo 415 Manual To Operations

NEW BRUNSWICK SCIENTIFIC BioFlo 415 Manual To Operations (164 pages)

Benchtop SIP Fermentor

Brand: NEW BRUNSWICK SCIENTIFIC | Category: Test Equipment | Size: 6.66 MB

Table of Contents

Table of Contents
7
1 Warnings & Cautions

13
- Warnings
  13
- Cautions
  13
2 Inspection & Unpacking of Equipment

15
- Inspection of Box(Es)
  15
- Packing List Verification
  15
- Basic Components
  15
- Box(E)
  15
3 Introduction & Overview

16
- System
  16
- Vessels
  16
- Agitation System
  16
- Temperature Control
  17
- Aeration
  17
- Ph Control
  17
- Do Control
  18
- High Foam Control
  18
- Exhaust System
  18
- Sampling/Harvesting System
  18
- Recommended Accessories & Supplies
  19
- Supervisory Software
  19
4 Installation

20
- Physical Location
  20
- Environment
  20
- Installing the Control Cabinet
  20
- Installing the Touchscreen
  20
- Utilities
  22
  - Electrical Requirements
    23
  - Water and Drain Connections
    24
  - Gas Connections
    24
  - Exhaust Connection
    24
- Vessel Assembly
  25
  - Sparge & Exhaust Assemblies
    27
  - Other Ports & Plugs
    29
  - Insert Probes
    29
  - Install Motor Assembly
    30
  - Connecting Vessel to Cabinet
    30
- Electrical Connections
  33
- Main Power Switch
  33
- Optional Biocommand Plus
  34
- Inputs/Outputs for Ancillary Devices
  34
5 Specifications

36
- Certifications
  37
6 Operating Controls

39
- Touchscreen
  39
- Display Screens
  39
  - Touchscreen Calibration
    39
  - Start-Up Screen
    40
  - Summary Screen
    40
  - Synoptic Screen
    42
  - Gauge Screens
    43
  - Adding Loops
    44
  - Deleting Loops
    45
  - Selecting Loop Control Modes
    46
  - Calibration Screen
    46
  - Cascade Screen
    47
  - Trend Screen
    47
  - Pump Screen
    48
  - Alarm Summary Screen
    48
  - Sterilization Screen
    49
  - Setup Screen
    50
- Rs232/422 Computer Interface
  51
7 Probe Preparation & Calibration

54
- Ph Probe Inspection
  54
- Ph Probe Calibration
  54
  - Ph Probe Installation
    56
  - Ph Probe Maintenance & Storage
    57
- Dissolved Oxygen (Do) Probe Preparation
  57
  - Inspecting the DO Probe
    57
  - DO Probe Preparation
    58
  - DO Probe Installation
    58
  - DO Probe Polarization
    59
  - DO Probe Calibration: Setting Zero
    59
  - DO Probe Calibration: Setting Span
    60
8 Automatic Sterilization

61
- Essential: Backflow Prevention Kit
  61
- Bioflo 415 • M1360
  62
- Sterilization
  63
- Valve Sequencing
  68
- Sterilization Phase Indication
  70
- Aborting Sterilization
  70
9 Preparing for Fermentation

71
- Systems Check
  71
- Adding Media to the Vessel
  72
10 Getting Started

73
- Control Modes
  73
- Pump Assignment
  73
- Settingp & I Values
  74
- Loop Setpoints
  74
  - Entering Setpoints
    74
  - Modifying Setpoints
    75
- Do Cascade System
  75
- Using Level Probes to Program Feed Pumps
  76
  - Setting a Feed Pump to Add Liquid
    76
  - Setting a Feed Pump to Harvest
    76
  - Using Level Probe to Program Feed Pump
    76
  - Level Control off
    77
  - Pump Calibration
    77
  - Exhaust Heater
    78
11 Cascade Control

79
- Creating a Cascade
  79
- Controlling Do by Cascade
  81
12 Plotting Trends

84
- Creating a Trend Graph
  84
  - Using the Export Button
    86
  - Using the Zoom Button
    87
  - Using the Read Line
    87
13 About Pumps

89
- Pump Setpoint
  89
- Pump Control Mode
  90
- Pump Flow Rate & Calibration Methods
  91
- Pump Period
  92
- Installing an External Variable Speed Pump
  92
14 About Alarms

94
- Abs and Dev Alarms
  94
- Setting Alarms
  94
- Acknowledging an Alarm
  96
- Alarms History
  97
15 Using the Setup Screen

99
- Controller Setup
  99
  - Gas Control with 1 or no TMFC
    101
  - Gas Control with 2 Tmfcs
    101
  - Gas Control with 3 Tmfcs
    102
  - Gas Control with 4 Tmfcs
    103
  - R Ecipe M Anager
    104
- Recipe
  105
- System Settings
  105
  - Resetting Date/Time
    106
  - Updating Software
    106
- Hardware Setup
  106
- Security Settings
  108
16 Operation

110
- Load Pump Tubing
  110
- Install Liquid Addition Systems
  112
  - Addition Tubing Size
    113
- Inoculation
  114
- Launch Biocommand (if Present)
  114
- Sampling Procedure
  114
- Harvesting
  116
- Fermentation Phases
  116
  - Phase
    116
  - Decline Phase
    117
  - Exponential Growth Phase
    117
  - Lag Phase
    117
  - Steady State Phase
    117
- Batch Operation
  117
- Fed Batch Operation
  117
- Chemostat Operation
  118
- Shutdown Procedure
  119
17 Cleaning

120
- Cleaning the Vessel
  120
  - List of Wetted Parts
    120
- Cleaning the Cabinet
  121
18 Maintenance

122
- Ph Probe Maintenance and Storage
  122
- Do Probe Maintenance and Storage
  122
- Vessel & Tubing
  123
- Periodic Inspection
  123
- Fuse Replacement
  123
- Port & Adapter O-Rings
  123
- Headplate O-Ring Replacement
  123
- Replacement Parts
  124
- Part
  124
19 Service

125
- Troubleshooting
  125
- Service Parts
  127
- Part
  127
20 Drawings

128
- List of Drawings
  131
- List of Tables
  133
  - Table
    133
21 Appendix A: some General Concepts

134
- What Is a Controller
  134
- What Is a Control Loop
  134
- What Is Probe Calibration
  135
- What Are P-I-D Constants
  135
- What Is P-I-D Tuning
  136
- What Do the Constants Mean
  137
22 Appendix B: Otr

138
- Determining an Oxygen Transfer Rate
  138
  - OTR Calculations
    138
- Some Factors that Affect Otr and Horsepower
  139
23 Appendix C: Fermentation Techniques

140
- Media Formulation
  140
- Antifoam Formulation
  141
- Tubing Size
  141
- Acid & Base
  142
- Glucose Feed
  142
- Recommended Process Control Settings
  143
24 Appendix D: Corrosion Resistance Tables

144
25 Appendix E: General Characteristics of Epr

159
- Identifying Epr
  159
- General Characteristics
  159
26 Index

160

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