Software; What Types Of Liquid Transfers Are Supported - Labcyte Echo 525 User Manual

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CHAPTER 5 | Software

5 | Software

This chapter describes using the Echo Liquid Handler. The following procedures are described:

What types of liquid transfers are supported?

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Supported Labware
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Startup/Shutdown
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Echo Liquid Handler Software
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What types of liquid transfers are supported?
The Echo Liquid Handler supports a variety of simple to complex microplate liquid transfer protocols. The bundled Echo Liquid
Handler software allows a user to set up the following types of protocol transfers:
Table 9: Liquid Transfer Types
Source (Src) Destination (Dest)
Microplate Microplate
384-well 96-well
384-well 384-well
384-well 1536-well
1
Destacking: transferring liquid from a single source (src) plate to several destination (dest) plates
2
Stacking: transferring liquid from several source plates to a single destination plate
Note: The Echo 525 Liquid Handler client software that comes standard with the Echo instrument is designed for 384-
well and 1536-well plate replication protocols. However, the Echo 525 Liquid Handler software does not currently
support the 6-well Echo Qualified Reservoir plate. To use the 6-well Echo Qualified Reservoir plate that is configured on
some Echo 525 Liquid Handlers, one of the following applications must be installed on the client: Echo Plate Reformat
application, Echo Cherry Pick application, Echo Dose Response application. For more information, see the Echo 525
Liquid Handler - Echo Qualified Reservoir Quick Start Guide.
Liquid transfer can be tailored to the user's specific requirements. Variables that are user selectable or definable include the
following:
Source microplate type (from the list of qualified plates)
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User-definable destination microplate or surface
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Transfer type (for example, interleaved or quadrant 384-well to 96-well transfers)
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Transfer volume
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Number of copies (how many times the transfer protocol is executed)
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Transfer map specifying exactly which wells, or regions of wells, will be transferred
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For information on these fluid transfer attributes and choices, see
Examples of these protocols are described later in this section. Additional and more complex fluid transfer protocols can be
defined with the Echo Applications software packages (Echo Plate Reformat, Echo Cherry Pick, Echo Dose-Response, Echo Plate
Audit, Echo Array Maker, and Echo Combination Screen), as well as with the extensive ActiveX control library defined in the Echo
Software Development Kit (Echo SDK). These protocols can specify virtually any combination of transfers from source microplate
wells to destination wells and slides. Refer to the Echo Application guides and the Echo Integration Guide for more information.
USER GUIDE|Echo® 525 Liquid Handler User Guide
Transfer Map Type (Full)
1
destacking quadrant (4 regions from 1 src
plate to 4 dest plates)
standard (1 src plate to 1 dest plate)
2
stacking quadrant (4 src plates to 4 regions
on 1 dest plate)
destacking interleaved (2x2, or every
other dest well)
standard (1 src plate to 1 dest plate)
stacking interleaved (2x2, or every
other dest well)
Echo Liquid Handler Software.
Transfer Map Type (Partial)
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