Cycle Fluid Through Echo System - Labcyte Echo 525 User Manual

USER GUIDE | Echo® 525 Liquid Handler User Guide
Compressed air or vacuum with brush
l
Follow the steps below to clean the destination plate gripper grid:
1. Display the Diagnostics window in the Echo Liquid Handler software.
2. Click Door Open to open the door.
3. Click Destination Plate Out. The software extends the destination plate gripper and automatically turns off the electrical
current.
Figure 110: Destination plate gripper extended
4. Gently remove any dust or particles on the grid with compressed air or vacuum with brush.
5. Click Flip Destination Plate Down and repeat the previous step to clean the grid.
6. Click Destination Plate In and select None from the Plate Name drop-down menu in the Retract Dest Plate Gripper dialog
box. The software retracts the destination plate gripper.

Cycle Fluid Through Echo System

Follow the steps below to cycle fluid through the Echo system:
Caution: The chiller does not need to be running for this procedure, however, do not cycle coupling fluid through the
Echo Liquid Handler that is below 22°C or above ambient room temperature. Extreme temperatures can damage the
acoustic transducer.
1. Turn on the coupling fluid pump. The coupling fluid will be pumped into the Echo Liquid Handler. After three to five
minutes, verify that the coupling fluid is returning to the coupling fluid bottle and that it does not contain a large number of
bubbles.
2. To completely purge the Echo fluidic circuit, repeat the purge, clean, and refill procedure two more times. For more
information, see Purge Fluid Circuit
3. After filling the fluidic circuit, the coupling fluid bottle will be partially empty. Turn the coupling fluid pump off, and add
coupling fluid so that the bottle contains 900 mL of coupling fluid. Make sure that the J-shaped tube isn't covered by the
new fluid level.
4. Turn on the coupling fluid pump.
5. Check the Coupling Fluid status on the Echo Liquid Handler software Diagnostics window. The indicator should be green.
159
and Echo Coupling Fluid Maintenance.
Maintenance Procedures
PN | 001-11665