Required Materials And Equipment - Thermo Scientific Ion Torrent S5 User Manual

Required materials and equipment

Table 1 Required materials and equipment for sequencing
™
Ion Chip Minifuge (120 V or 230 V)
™
Ion Proton Rotor and Buckets Kit
Uninterruptable Power Supply (UPS)
Isopropanol (100%)
Nuclease-free water molecular biology grade
[3]
Microcentrifuge
P2, P10, P20, P200, P1000 µL pipette set and filtered tips
® ®
Rainin Pipet-Lite XLS with RFID LTS 2 µL to 20 µL
® ®
Rainin Pipet-Lite XLS with RFID LTS 10 µL to 100 µL
® ™
Rainin StableRak LTS tips 2-20 µL
® ™
Rainin StableRak LTS tips 200 µL
0.2-mL MAXYMum Recovery
Cap (do not use polystyrene tubes)
1.5-mL or 1.7-mL microcentrifuge tubes
Ice buckets and ice
Aluminum heat block, capable of maintaining 50°C
Vortex mixer with a rubber platform
Thermal cycler with a heated lid
Standard laboratory vacuum line or vacuum pump
Liquid trap
® [4]
Tygon tubing
[1]
Thermo Fisher Scientific has validated this protocol using these specific materials. Substitution may adversely affect system performance.
[2]
For laboratories that experience frequent power outages or line voltage fluctuations, we recommend that you use an uninterruptable power
supply that is compatible with 2500 W output or higher.
[3]
Must fit standard 1.5- and 0.2-mL microcentrifuge tubes and generate 15,500 ×
[4]
As needed to connect laboratory vacuum to liquid trap and liquid trap to P200 pipette tip.
Ion Torrent S5 ™ andIon Torrent S5
CONFIDENTIAL — For Life Technologies Internal Use Only. Do Not Distribute.
The following tables list required materials and equipment that are not provided with
™
the Ion Torrent S5 Systems.
[1]
Description
[2]
®
Thin Wall PCR Tubes, Flat
™ XL Instrument User Guide
Chapter 1 Product information
Required materials and equipment
Supplier Catalog no.
Life 4479672 (120V)
Technologies or
4479673 (230V)
Life 4482578
Technologies
MLS —
MLS —
MLS —
MLS —
MLS —
Rainin L-20XLS
Rainin L-100XLS
Rainin SR-L20F
Rainin SR-L200F
Axygen PCR-02-L-C
MLS —
— —
MLS —
MLS —
MLS —
MLS —
MLS —
MLS —
g .
1
Quantity
1
1
1
—
—
1
1 set
1
1
—
—
Varies
Varies
—
—
1
1
1
1
1
7