Gilson Pipetman Ultra U2 User Manual page 11

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You may wipe any droplets away from the outside
of the tip using a medical wipe, however if you
do so take care to avoid touching the tip's
orifi ce.
5) Dispense.
Place the ends of the tips against the inside
walls of the recipient vessels (at an angle of 10°
to 40°).
Press the push-button slowly and smoothly to
the fi rst stop.
Wait for at least a second, then press the push-but-
ton to the second stop to expel any residual liquid
from the tips. Keep the push-button pressed fully
down and (while removing the pipette) draw the
tips along the inside surface of the vessel.
Release the push-button, smoothly.
6) Eject the tips by pressing fi rmly on the tip ejector
button.
The tip ejector stroke can be set to allow for diff erent
types of tip, where (for example) the length of the collar
is diff erent (refer to "Personalizing Your Pipette").
General Guidelines for Good Pipetting
1) Make sure that you operate the push-button
slowly and smoothly.
2) When aspirating, keep the tips at a constant
depth below the surface of the liquid (refer to
the table "Immersion Depth and Wait Time").
3) Change the tips before aspirating a diff erent
liquid, sample, or reagent.
4) Change the tips if droplets remain at the end of
the tip from the previous pipetting operation.
First Stop Second Stop
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