Gilson pipetman User Manual
  1. Manuals
  2. Brands
  3. Gilson Manuals
  4. Laboratory Equipment
  5. pipetman
  6. User manual

Gilson pipetman User Manual

Hide thumbs Also See for pipetman:
Table of Contents

Advertisement

Quick Links

See also: Quick Manual
Progress made simple!
USER'S GUIDE
&
SINGLE
MULTICHANNEL

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the pipetman and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Gilson pipetman

Summary of Contents for Gilson pipetman

  • Page 1 Progress made simple! USER’S GUIDE & SINGLE MULTICHANNEL...

  • Page 2: Table Of Contents

    10 - PERSONALIZE YOUR PIPETMAN M 11 - GOOD LABORATORY PRACTICE FEATURES 12 - PIPETMAN M POWER MANAGEMENT 13 - CONFIGURATION 14 - MAINTENANCE OF YOUR PIPETMAN M 15 - CLEANING AND DECONTAMINATION 16 - LEAK TEST 17 - TROUBLESHOOTING...

  • Page 3: Introduction

    PIPETMAN M is so easy to use that you will have the feeling that you are using a PIPETMAN Classic: • Push-button: set the volume by turning the button left or right and pipette with a simple push.

  • Page 4: Parts Check List And Accessories

    P8x200M, P12x200M, P8x300M, P12x300M)). GETTING STARTED PIPETMAN M i s p rovided w ith a m inimal b attery c harge. B efore u sing y our n ew PIPETMAN M, we strongly recommend you to fully charge the battery. PIPETMAN M charges 80 % of its full battery capacity in less than an hour, it takes three hours to fully charge the battery. Please refer to PIPETMAN M Power Management (Chapter 12) for charging procedure.

  • Page 5: Description

     and mode-  button for at least 5 seconds  Switch Off:  To switch off your PIPETMAN M, press the push-button  for at least 5 sec. Display The PIPETMAN M display is an OLED screen. Usually, it shows the current mode and opera-...

  • Page 6: Pipetting Just Like With Pipetman

    PIPETTING JUST LIKE WITH PIPETMAN Using PIPETMAN M is as easy as using a mechanical PIPETMAN. Your PIPETMAN M is set by default on “PIPET Mode”, that means forward pipetting. You can select a volume and start to pipette. Switch on Press push-button.

  • Page 7: Pipetting Modes

    ENGLISH This feature enables you to remove the tip from the liquid, without aspirating anything. • Eject tip by pressing the ejection button. Your PIPETMAN M is ready again for next pipetting cycle while maintaining the volume and the last settings! You have been pipetting like with your mechanical PIPETMAN and only needed 2 pushes on the push-button! PIPETTING MODES Mode-button PIPETMAN M offers more pipette techniques for a large number of applications: You will find all pipetting modes in the menu (for system settings refer to the Chapters 7 and 13). Access the menu by pushing the...
  • Page 8 1. Fit a tip suitable for the model of PIPETMAN M that you You must always fit a tip are using (preferably use PIPETMAN TIPS for assurance of before using any pipette! accurate and precise results; refer to chapter 8). 2. Click push-button to aspirate total DISCARD...
  • Page 9 If you set the VOLUME 3 = 0 µL the cycle will stop after the MIX step. You are ready to mix! 1. Fit a tip suitable for the model of PIPETMAN M that you You must always fit a tip are using (preferably use PIPETMAN Tips for assurance of before using any pipette! accurate and precise results; refer to chapter 8).
  • Page 10 The REVERSE Mode is ideal for pipetting viscous liquids; liquids with high vapour pressure or those which tend to foam. Your PIPETMAN M aspirates a selected volume and an excess. This excess compensates for the liquid that remains as a film inside the tip during dispensing. For example: protein extraction, cell lysis, plasmid isolation, cell culture, buffer preparation and many others…...

  • Page 11: Pipetting Speed Control

    ENGLISH PIPETTING SPEED CONTROL PIPETMAN M is set by default on speed 6. You may need to change the speed of aspiration or dispensing depending on your application. You can change aspiration speed and dispensing speed independently of one other: from very slow to very fast (speed 1 – speed 6). Your speed selection is memorized for each pipetting mode until you change it again.

  • Page 12: Guidelines For Good Pipetting

    Pipette model Tips model Range Color code a lack of precision. P10M D10* 0.1 – 10 μL • PIPETMAN TIPS (except filter tips) may be auto- DL10 0.1 – 20 μL claved at 121°C for 20 minutes at 0.1 MPa. DF10 0.1 – 10 μL DFL10 0.1 – 10 μL...
  • Page 13 P8x200M, P12x200M f. Liquid should never enter the tip holder. To prevent this: P8x300M, P12x300M • Never turn the pipette upside down, • N ever lay the pipette on its side when there is liquid in the tip(s), g. The Gilson Stand Adapter (see Chapter 2) is recommended for use with the CARROUSEL and SINGLE Pipette Holder to store your PIPETMAN M pipette in the vertical position. Alternatively the POWER CARROUSEL can be used to store and charge up to 5 PIPETMAN M pipettes. h. When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip several times before use in order to reach equilibrium between the temperatures of the liquid and the pipette’s dead-volume.

  • Page 14: Personalize Your Pipetman M

    Short tips Long tips Personal Label – Name and/or Application Tag: Your PIPETMAN M offers you two options to mark your pipette. You can personalize your pipette with a name tag and/or an application tag: Window-Tag: • Remove the window by inserting a small screwdriver in the access slot.

  • Page 15: Good Laboratory Practice Features

    • Service is overdue warning (see Chapter 14). • Service Alert can be set “On” or “Off”. PIPETMAN M POWER MANAGMENT The PIPETMAN M battery has been designed to ensure 900 pipetting cycles (750 for PIPETMAN M Multi 200 µL and 300 µL) at maximum speed (up to nine 96 well plates) between two charge cycles. For ser- vice continuity when needing more capacity, your PIPETMAN M can also be used while charging with the same performance as a fully charged pipette! USB port Battery Charging PIPETMAN M charges 80 % of its full battery capac-...
  • Page 16 Low Battery Warning: PIPETMAN M has a low battery alert. As the battery runs down, the LOW BATTERY message appears.The warning is replaced by a blinking symbol of an empty battery. If it is not recharged, the pipette will switch off after a while.

  • Page 17: Configuration

    ENGLISH - PIPETMAN M battery won’t charge or will not operate even if connected to the charger at least for 10 minutes. - PIPETMAN M battery provides you very short cycle time or permanently needs to be used in con- nected mode.

  • Page 18: Maintenance Of Your Pipetman M

    Service Information PIPETMAN M provides you with all required service information. It will help you establish an easy diagnosis and plan any service operation with your accredited Gilson Service Center. You will find in the “service” menu following sub menus: a. Disassembly b. Service Info c. Service Settings d. Pipette Info The navigation of the menus is always done in the same way ...
  • Page 19 You may want to calibrate your pipette for solutions with a density, viscosity, surface tension or vapour pressure that are different to that of water. To return to the factory settings, choose “Reset Settings”, your PIPETMAN M will be reset to the three factory calibration values which are permanently stored in the pipette’s firmware.
  • Page 20 Maintenance Warning PIPETMAN M notifies you, when maintenance is due (service alert is set by default to “ON”). The following message will appear: after validation by a click, the following message appears: If you click to confirm within 10 sec. the pipette will automatically switch to “disassembly” mode so you can carry out your maintenance safely.
  • Page 21 ENGLISH  Changing the seal and/or O-ring (see Chapter 20) The O-ring is positioned on the piston; it should not be autoclaved, if O-ring worn or damaged in any way, it must be replaced. To access the O-ring, remove the tip-ejector and unscrew the lower part of the tip-holder. You should now be able to remove the O-ring from the piston. Sometimes, the O-ring may be found in the recess at the top- end of the lower part of the tip-holder. If required, lubricate the piston (see “How to Lubricate the Piston”) then fit a new O-ring by sliding it onto the piston. Reassemble the pipette. Depending on the pipette model the dimensions of the O-ring will vary (refer to Chapter 20). ...

  • Page 22: Cleaning And Decontamination

    PIPETMAN M is designed so that the parts normally in Liquid must not enter the handle contact with contaminants can easily be cleaned and of the pipette! decontaminated. As part of your quality system, you may have procedures for pipette decontamination. We recom- mend that you refer to “Decontamination Procedures for Gilson Pipettes”, which you can download from Gilson’s website. - 22 -...
  • Page 23 Cleaning The pipette must be cleaned, as described below, before it is decontaminated. For cleaning your PIPETMAN M you may use a simple soap solution or any of the solutions mentioned in “Decontamination Procedures for Gilson Pipettes”. Single channel models ...
  • Page 24 Please note that although the lower part of PIPETMAN M Multichannel can withstand a few number of autoclaving cycles, we do not recommend autoclaving it. Decontamination Autoclaving - Single channel models However, after separation from the body, any of the The body (handle) of the pipette is following components of the volumetric module may...

  • Page 25: Leak Test

    • The water level inside the tip should remain constant; if the level goes down there is a leak (see Chapter 17). • For Multichannel models, check if the water level between each tip is the same. Before returning any pipette to your TROUBLESHOOTING local Gilson Service Center, ensure that it is completely free of chemical, biological, In case of malfunction, first reset the pipette by or radioactive contamination. Please use the included safety bag. pressing on the push-button and the Mode-button simultaneously at least for 8 seconds.

  • Page 26: Specifications

    SPECIFICATIONS PIPETMAN M is a high quality pipette that of- Maximum Permissible Errors fers excellent accuracy and precision; it is fully Model PIPETMAN Volume Gilson ISO 8655 compliant with ISO 8655 and is CE marked (for (Reference) tips used Systematic Random Systematic Random IVD and EMC directives). error (μL) error (μL) error (μL) error (μL) P10M Min. 0.5 ± 0.04 ≤ 0.013 ± 0.12 ≤ 0.08 The figures given in the “Gilson Maximum...

  • Page 27: Safety Precautions And Limitations Of Use

    For safety reasons, it is important to observe the following instructions: 1. Battery and electrical specifications: We strongly recommend you fully charge the battery before using the pipette. PIPETMAN M is supplied with an AC adaptor that is suitable for your country. You must only use an original Gilson AC adaptor specific to this product. Charge the battery in the pipette, using the AC-adapter or the stand adapter. Use AC-adaptor and stand adapter indoors.

  • Page 28: Replacement Parts

    9. PIPETMAN M can be used indoor and outdoor, if precautions are respected as described above in this handbook and in the GLP. REPLACEMENT PARTS Single channel models Description P10M P20M P200M P1000M Connecting nut F807012 F807012 F807012 F807012 Battery tag, 1x4 F807013 F807013 F807013 F807013 Ejector tag, 1x4...

  • Page 29: Appendices

    ENGLISH PPENDIX 1 - EXAMPLE OF A PERFORMANCE CHECK Below is an example of how to evaluate the performance of PIPETMAN P10M at 1 µL. 4. Calculate the mean volume 1. Determine the mean value e of the evapora- that occurs during your pipetting tion loss e V = ( W+e ) x Z cycles. Proceed as described in Appendix 3 to determine e (0.964+0.018) x 1.0032 0.985 µL For a temperature of 21.5 °C and an air pressure...
  • Page 30 PPENDIX 2 - Z FACTOR The reference calculation equation is : Where : P = density of air at t°C. Z = [1/(P )] [1-(P = density of the test liquid at t°C. = density of the balance weights. Use 8 g/cc for P  Weights conforming to International Recommendation N°33 of OIML have been adjusted to give results when weighing in air as if the density of the weights were 8.0 g/mL.
  • Page 31 ENGLISH PPENDIX 3 - EVAPORATION LOSS Procedure for the determination of evaporation loss Use the same distilled water, weighing vessel and balance as you will be using for the gravimetric check. 1. H alf fill the weighing vessel with distilled water. 2. C over the weighing vessel with its lid and place it on the balance using a pair of tweezers. 3. A spirate a sample. 4. T are the balance and take the weighing vessel out of the balance.
  • Page 32 PPENDIX 4 - HOW TO CALCULATE VOLUMETRIC ACCURACY AND PRECISION “Accuracy” and “precision” are qualitative terms. The corresponding quantitative terms are “systematic error” and “random error”. Evaluation of accuracy Evaluation of precision The specified precision is the limit to the random The specified accuracy is the limit to the system- atic error, which is the difference between the error, which is the distribution of the measured...

  • Page 33: Ec Declaration Of Conformity

    ENGLISH DECLARATION OF CONFORMITY GILSON S.A.S. 19, avenue des Entrepreneurs BP 145 - 95400 VILLIERS LE BEL The company FRANCE Tel.: +33(0)1 34 29 50 00 Fax: +33(0)1 34 29 50 20 PIPETMAN® M Hereby certifies on its sole responsibility that the P10M, P20M, P200M, P1000M products listed below: P8x20M, P12x20M P8x200M, P12x200M, P8x300M, P12x300M comply with the requirements of the following 98/79/EC* European Directives: In Vitro Diagnostic Medical Devices 2004/108/EC Electromagnetic Compatibility, EMC...
  • Page 34 NOTES - 34 -...

  • Page 35: Warranty

    This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes.
  • Page 36 LT801527/E- ©2013 Gilson SAS All rights reserved April 2013 English Printed in France Specifications subject to change without notifications - errors omitted. www.gilson.com sales@gilson.com service@gilson.com training@gilson.com Gilson, Inc. World Headquarters 3000 Parmenter Street P.O. Box 620027 Middleton, WI 53562-0027, USA...

Table of Contents

Save PDF