Gilson PIPETMAN P Series How To Use Manual

Gilson PIPETMAN P Series How To Use Manual

Micropipette
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How to use Micropipette: Pipetman P
Prepared for
Gilson Inc.
Prepared by
Doan Trang Nguyen
TECM-2700-001
Terry Smith
05/02/2014

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  • Page 1 ® How to use Micropipette: Pipetman P Prepared for Gilson Inc. Prepared by Doan Trang Nguyen TECM-2700-001 Terry Smith 05/02/2014...
  • Page 5: Table Of Contents

    Pre-rinse the tips ........................15 Aspirate ..........................15 Dispense ..........................16 General Guidelines ........................16 Specifications ..........................19 Gilson’s maximum Permissible Error Table ................19 GLP Features and Accessories ...................... 23 GLP Features ..........................23 Accessories ..........................23 Leak Test and Troubleshooting ....................27 Leak Test ...........................
  • Page 6 ® How to use Micropipette: Pipetman P Chemical Decontamination ....................... 35 Upper Part (Handle) ......................36 Lower Part (Volumetric module) ..................36 Spare Parts ............................ 39 Tables of spare parts for different models ................. 39 Index ............................. 40...
  • Page 7: Introduction

    Introduction Introduction Gilson Inc. designs and manufactures the PIPETMAN P following the Pipetting Standard to provide you with a range of robust, accurate and precise pipettes. This fully adjustable, air- displacement pipette comes with a digital indicator (volumeter) that shows the selected volume.
  • Page 9 Fly chapter 1...
  • Page 11: Part Checklist And Descriptions

    Checklist and Descriptions Part Checklist and Descriptions Part Checklist Just take a moment to verify that the package contains these following items:  PIPETMAN P  User's Guide  Safety bag  Certificate of conformity (including bar-code sticker). Descriptions Color-coded push-button Thumbwheel Tip-ejector...
  • Page 13 Fly chapter 2...
  • Page 15: Volume Setting

    Volume Setting Volume Setting You use the volumeter to set the liquid volume you need to aspirate. Depending on the model, we color the dial numbers in the digital display with black or white to indicate the position of the decimal point (see Figure You set the volume by turning the thumbwheel or the push-button.
  • Page 17 Fly page chapter 3...
  • Page 19: Pipetting And General Guidelines

    PIPETMAN P. We used pure polypropylene to make DIAMOND Tips and engraved the Gilson logo on their collar, ensuring that you have a genuine Gilson product. Only use each plastic tip for a single application. PIPETMAN P can also be used with the main tip brands.
  • Page 20: Dispense

    ® How to use Micropipette: Pipetman P 4. Wait one second (time depends on model, see table); then withdraw the pipette-tip from the liquid. 5. You may wipe any droplets away from the outside of the tip using a medical wipe, however if you do so take care to avoid touching the tip’s orifice.
  • Page 21 Fly page chapter 4...
  • Page 23: Specifications

    DIAMOND Tips to obtain the data in the “Gilson Maximum Permissible Errors” and we guarantee these data only with the usage of genuine Gilson DIAMOND Tips. Gilson’s qualified technicians inspected and validated each pipette in accordance with the Gilson Quality System.
  • Page 24 ® How to use Micropipette: Pipetman P  With a precise pipetting technique (see Chapter 6 - General guidelines for good pipetting) the P2 model may be used to aspirate volumes as low as 0.1 μL and the P10 model as low as 0.5 μL.
  • Page 25 Fly page chapter 5...
  • Page 27: Glp Features And Accessories

    The Bar Code on the box and the certificate of conformity provide traceability of your pipette. Figure 5 Accessories To make pipetting more comfortable and more secure, Gilson has developed several accessories: To avoid the possibility of liquid running back into the pipette, store the pipette vertically. ™  CARROUSEL Pipette stand (7 pipettes) –...
  • Page 29 Fly page chapter 6...
  • Page 31: Leak Test And Troubleshooting

    O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test. For the P2 to P200 models 1. Fit a Gilson DIAMOND Tip. Set the pipette to the maximum volume given in the specifications, and pre-rinse.
  • Page 32 Return pipette to supplier Damaged tip-holder(s) Replace the tip-holder Worn O-ring or seal Replace both parts Tips fall off or do not fit Low quality tips Use Gilson DIAMOND tips correctly Damaged tip-holder(s) Replace the tip-holder Damaged tip-ejector Replace tip-ejector...
  • Page 33 Fly page chapter 7...
  • Page 35: Maintenance

    After replacing any parts you should verify the performance of your pipette following the verification procedure available on the Gilson website (www. gilson.com). If the pipette needs to be readjusted, please contact your local Gilson authorized Service Center. Changing the Tip-ejector 1.
  • Page 36: Changing The O-Ring

    ® How to use Micropipette: Pipetman P 1. Remove the tip-ejector (see above). 2. Unscrew the connecting nut by turning it counter- clockwise. 3. Carefully separate the lower and upper parts. 4. Remove the piston assembly, O-ring and seal. 5. Clean and decontaminate the piston assembly. 6.
  • Page 37 Fly page chapter 8...
  • Page 39: Cleaning And Decontamination

    Cleaning and Decontamination Cleaning and Decontamination Gilson designed PIPETMAN P for easy cleaning and decontaminating the parts that normally in contact with liquid contaminants. However, because the models P2 and P10 contain miniatures parts, we recommend keeping the pipettes intact. Please contact your local Gilson authorized Service Center for further questions.
  • Page 40: Upper Part (Handle)

    ® How to use Micropipette: Pipetman P (Polyamide), PBT (Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephtalate), PoM (Polyoxymethylene), or PVDF (Polyvinylidene Fluoride). Upper Part (Handle) 1. Wipe the upper part (handle) of the pipette with a soft-cloth or lint-free tissue impregnated with the chosen decontaminant.
  • Page 41 Fly page chapter 9...
  • Page 43: Spare Parts

    Spare Parts Spare Parts The micropipette package comes with two levels of service kit generalized for all models in the package. 1. Service Kit 1 level:  3 piston seals (C)  3 O-rings (D)  1 tip-holder (E) 2. Service Kit 2 level: ...
  • Page 44: Index

    P2, 3, 15, 20, 27, 31, 35, 39 DIAMOND Tips, 15, 19 P20, 3, 39 Dispense, 16 P200, 3, 27, 39 Gilson authorized Service Center, 27, 31, 35 P5000, 3, 39 ISO 8655, 16, 19, 20 PIPETMAN P, 3, 7, 15, 19, 35 ISO 8655-2, 16...

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