14 - sPaRe PaRTs eC DeCLaRaTION Of CONfORMITY 1 - IntroductIon The Pipetting Standard – Gilson's PIPETMAN Classic is designed and manufactured to provide you with a range of robust, accurate and precise pipettes. The PIPETMAN Classic is a fully adjustable, air-displace- ment pipette with the selected volume shown on a digital indicator (volumeter).
2 - Parts check LIst Just take a moment to verify that the following items are present: - PIPETMAN Classic, - User's Guide, - Safety bag, - Certificate of conformity (including bar-code sticker). 3 - descrIPtIon Please refer to the following chapters for a full description of the different parts and functions of the pipette.
4 - settIng the VoLume The volume of liquid to be aspi- rated is set using the volumeter. The dials are colored either black or red to indicate the 1.25 µL 7.5 µL 12.5 µL position of the decimal point, depending on the model (see P100 P200...
DIAMOND Tips with your PIPETMAN Classic is strongly recommended. These tips, made from pure polypropylene have the Gilson logo engraved on their collar, ensuring that you have a genuine Gilson product. Plastic tips are for a single application – they must not be cleaned for reuse.
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aspirate Press the push-button to the first stop (this corresponds to the set volume of liquid). Hold the pipette vertically and immerse the tip in the liquid (see immersion depth table, page 7). Release the push-button slowly and smoothly (to top posi- tion) to aspirate the set volume of liquid.
Release the push-button, smoothly. Eject the tip by pressing firmly on the tip-ejector button. 6 - generaL guIdeLInes For good PIPettIng Make sure that you operate Table - Immersion Depth and Wait Time the push-button slowly and smoothly. Model Immersion Wait Time Depth (mm) (seconds) When aspirating, keep the tip at a constant depth...
7 - accessorIes To make pipetting more comfortable and more secure, Gilson has developed several accessories: To avoid the possibility of liquid running back into the pipette, store the pipette vertically. CaRROUseL™ Pipette stand (7 pipettes) f161401 TRIO™...
Refer to Chapter 12 - Cleaning and Decontamination. Please use the included safety bag to return the pipette to your local Gilson Service Center. The following table may help you to identify and correct the problem you might encounter.
The ejector lock is misaligned align the ejector lock However, if you can’t solve the problem, contact your Gilson representative. 10 - Leak test This test may be performed at any time to check that the pipette does not leak, especially after performing a main- tenance or decontamination procedure.
After replacing any parts you should verify the performance of your pipette following the verification procedure available on the Gilson website (www.gilson.com). If the pipette needs to be readjusted, please contact your local Gilson authorized Service Center.
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Piston assembly You may remove the piston assembly for cleaning pur- poses only. If the piston assembly is changed, the pipette must be adjusted and calibrated in a Gilson authorized Service Center. The piston assembly must not be autoclaved.
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Tighten the connecting nut (turn clockwise). Refit the tip-ejector (see above). Color coded push-button Connecting nut Piston assembly seal O-ring Tip-holder Tip-ejector...
Gilson authorized Service Center. You may refer to the decontamination procedure available on the Gilson website (www. gilson.com). Liquid must never enter the upper part (handle) of any pipette. cleaning The pipette must be cleaned, as described below, before it is decontaminated.
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tissue impregnated with soap solution, to remove all dirty marks. If the pipette is very dirty, a brush with soft plastic bristles may be used. Wipe the entire pipette and the tip-ejector with a soft cloth or lint-free tissue soaked with distilled water. Refit the tip-ejector and allow the pipette to dry.
13 - sPecIFIcatIons PIPETMAN Classic is a high quality pipette that offers excellent accuracy and precision. The figures given in the “Gilson Maximum Permissible Errors” table (page 17) were obtained using PIPETMAN DIAMOND Tips. These figures are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.
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Permissible errors Single channel Maximum Permissible Errors Model Volume Gilson ISO 8655 (Reference) (µL) systematic Random systematic Random error (µL) error (µL) error (µL) error (µL) ± 0.024 0.012 ± 0.08 0.04 (f144801) ± 0.025 0.012 ±...