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Summary of Contents for Gilson PIPETMAN P2L
- Page 1 User’s Guide Variable volume single and multichannel models and fixed volume models...
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Page 2: Table Of Contents
TABLE OF CONTENTS INTRODUCTION | 3 Parts Checklist | 3 GLP Features | 3 Description | 4 Specifications | 5 SETTING THE VOLUME (Variable Volume Models Only) | 7 USER ADJUSTMENTS (Fixed Models Only) | 7 PIPETTING | 8 Fit the Tips | 8 Pre-Rinse the Tips | 9 Aspirate | 10 Dispense | 10... -
Page 3: Introduction
INTRODUCTION PIPETMAN® L is an air displacement pipette and is used with disposable tips. To answer the current needs of intensive use of pipettes, and still being conform to Gilson brand qualities, improvements have been implemented to our mechanical series: •... -
Page 4: Description
Description Please refer to the following chapters for a full description of the different parts and functions of the pipette. Variable volume models Fixed models Single channel Multichannel Color-coded Color-coded push button push button Graduated cover Thumbwheel Thumbwheel Tip ejector Tip ejector button button... -
Page 5: Specifications
PIPETMAN DIAMOND Tips are used. Each pipette is inspected and validated by qualified technicians in accordance with the Gilson Quality System. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard, by type testing. - Page 6 FIXED MODELS GILSON ISO 8655 MODEL (µL) SYSTEMATIC RANDOM SYSTEMATIC RANDOM ERROR ERROR ERROR ERROR F1L (FA10017) ±0.020 ≤0.015 ±0.05 ≤0.05 F2L (FA10018) ±0.050 ≤0.020 ±0.08 ≤0.04 F5L (FA10019) ±0.05 ≤0.025 ±0.125 ≤0.075 F10L (FA10020) ±0.060 ≤0.030 ±0.12 ≤0.08 ≤0.050 ≤0.1...
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Page 7: Setting The Volume (Variable Volume Models Only)
Chapter 2 SETTING THE VOLUME This section applies to variable volume models only. The volume of liquid to be aspirated is set using the Multichannel Single channel models volumeter. The dials are colored either black or red to models indicate the position of the decimal point, depending on the model (refer to Figure 4). -
Page 8: Pipetting
To adjust the volume setting to compensate for a Clockwise specific density or viscosity, engage the two hooks of the spanner tool inside the two small holes on the top of the body, then turn the key slowly: • Clockwise to decrease the volume; making sure Graduations not to overshoot the mark. -
Page 9: Pre-Rinse The Tips
P2L and P10L models are delivered with the adapter in place, positioned in the longer slot, and ready to use DL10 tips. When D10 tips (which are shorter) are used, the adapter must be repositioned in the shorter slot as follows: Pull the adapter down from the stainless steel tip ejector. -
Page 10: Aspirate
Keep the push button pressed fully down and (while removing the pipette) draw the tip along the inside surface of the vessel. Release the push button, smoothly. Eject the tip by pressing firmly on the tip ejector button. For the multichannel models, use a Gilson reagent reservoir (refer to ACCESSORIES on page 11). -
Page 11: Accessories
Chapter 5 ACCESSORIES To make pipetting more comfortable and more secure, Gilson has developed several accessories: Pipette stands allow users to store pipettes vertically Disposable Reagent Reservoirs are ideal for to avoid the possibility of liquid running back into the dispensing reagent with multichannel pipettes: pipette. -
Page 12: Troubleshooting
You may download the 2 Minute Inspection Poster from the Gilson website (www.gilson.com), NOTE which shows how to perform a quick diagnosis of your pipette. Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of chemical, biological, or radioactive contamination. Refer to WARNING CLEANING AND DECONTAMINATION on page 17. -
Page 13: Leak Test
Leak Test This test may be performed at any time to check that the pipette does not leak, especially after performing a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test. For the P2L to P200L Single channel models, and the F1L to F250L Fixed models: Fit a PIPETMAN DIAMOND Tip. -
Page 14: Maintenance For Single And Fixed Models
Greasing the piston assembly. PIPETMAN P2L and P10L, F1L, F2L, F5L and F10L should not be disassembled, so you may only replace the push button, tip ejector, dual position tip ejector and its adapter. With these pipettes if the tip holder is damaged, the piston may also be damaged. -
Page 15: Service The Piston Assembly
You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the pipette must be adjusted and calibrated in a Gilson-authorized Service Center. As the models P2L and P10L, F1L, F2L, F5L and F10L contain miniaturized parts, it is best not to disassemble these pipettes yourself. -
Page 16: Change The Seals
As the models P2L and P10L, F1L, F2L, F5L and F10L contain miniaturized parts, it is best not to disassemble these pipettes yourself, please contact your local Gilson-authorized Service Center. The dimensions of the O-ring vary depending on the model of pipette. -
Page 17: Cleaning And Decontamination
However, because the models P2L and P10L, F1L, F2L, F5L, and F10L contain miniaturized parts, it is best not to disassemble these pipettes yourself; please contact your local Gilson authorized Service Center. You may refer to the decontamination procedure available on the Gilson website NOTICE ). -
Page 18: Chemical Decontamination
2. Procedure PIPETMAN L variable volume models can be sterilized by steam autoclaving at 121°C (252°F), 1 bar relative pressure for 20 minutes without disassembly. When autoclaving, the pipette will dry better and faster without using a bag. 3. After autoclaving Following the autoclaving cycle, leave the pipette to cool down to room temperature and dry completely before use. -
Page 19: Spare Parts
Chapter 10 SPARE PARTS For Variable Volume Single Models ► Service Kit 1 level includes: ○ Three piston seals or seal guides ○ Three O-rings ○ One tip holder ► Service Kit 2 level includes: ○ One push button ○ One connecting nut ○... - Page 20 For Variable Volume Multichannel Models Tip ejector (12 channels) Tip ejector (8 channels) Ejector lock Ejector spacer (8 channels) Ejector spacer (12 channels) Ejector spacer D10 (12 channels) Ejector spacer D10 (8 channels) P8X10L (FA10013) AND P12X10L (FA10014) LABEL DESCRIPTION 8X10 12X10 F3 - F4 Tip ejector...
- Page 21 For Fixed Volumes Single Models ► Service Kit 1 level includes: ○ Three piston seals or seal guides ○ Three O-rings ○ One tip holder ► Service Kit 2 level includes: ○ One push button ○ One connecting nut ○ One tip ejector F1 F2 ►...
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Page 22: Warranty
Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes. - Page 23 NOTES PIPETMAN L® USER’S GUIDE...
- Page 24 T: 608-836-1551 or 800-445-7661 • F 608-831-4451 Gilson S.A.S. 19, avenue des Entrepreneurs BP 145 • F-95400 Villiers-le-Bel, France T +33 (0) 1 34 29 50 00 • F +33 (0) 1 34 29 50 20 LT801575/B | ©08/2017 Gilson, Inc. All rights reserved.
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