Related Manuals for Gilson PIPETMAN Neo P2N
Summary of Contents for Gilson PIPETMAN Neo P2N
- Page 1 The Pipetting Comfort with Improved Comfort! IMPORTANT NOTICE! Do not lubricate this pipette. The use of lubricant cancels the warranty of this pipette.
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Page 2: Table Of Contents
Contents Page INTRODUCTION PaRTs CheCk LIsT DesCRIPTION seTTING The VOLUMe PIPeTTING GeNeRaL GUIDeLINes fOR GOOD PIPeTTING aCCessORIes GLP feaTURes TROUbLeshOOTING 10 - Leak TesT 11 - MaINTeNaNCe 12 - CLeaNING aND DeCONTaMINaTION 13 - sPeCIfICaTIONs 14 - sPaRe PaRTs eC DeCLaRaTION Of CONfORMITY WaRRaNTY 1 - IntroductIon PIPETMAN Neo is a fully adjustable air displacement... -
Page 3: Parts Check List
2 - Parts check LIst Just take a moment to verify that the following items are present: - PIPETMAN Neo, - User's Guide, - Safety bag, - Certificate of conformity (including bar-code sticker). 3 - descrIPtIon Please refer to the following chapters for a full description of the different parts and functions of the pipette. -
Page 4: Setting The Volume
Color-coded push-button Tip-ejector button Thumbwheel handle Connecting nut ejector lock ejector clip ejector support Tip-holders Cover Tip-ejector ejector spacer PIPeTMaN DIaMOND tips 4 - settIng the VoLume P10N P20N The volume of liquid to be aspi- rated is set using the volumeter. 1.25 µL 7.5 µL 12.5 µL... -
Page 5: Pipetting
These Tips, made from pure polypropylene have the Gilson logo engraved on their collar, ensuring that you have a genuine Gilson product. Plastic tips are for a single application – they must not be cleaned for reuse. PIPETMAN Neo can also be used with the main tip brands. - Page 6 P2N and P10N models are delivered with the adapter in place, positioned in the longer slot, ready to use DL10 tips. When D10 tips (which are shorter) are used, the adapter must be repositioned in the shorter slot as follows: Pull the adapter down from the metallic rod.
- Page 7 Pre-rinse the tips Some liquids (e.g. protein-containing solutions and organic solvents) can leave a film of liquid on the inside wall of the tip ; pre-rinse the tip to minimize any errors that may be related to this phenomenon. Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel (or to waste).
- Page 8 aspirate Press the push-button to the first stop (this corresponds to the set volume of liquid). Hold the pipette vertically and immerse the tip in the liquid (see immersion depth table, page 10). Release the push-button slowly and smoothly (to top posi- tion) to aspirate the set volume of liquid.
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Page 9: General Guidelines For Good Pipetting
Release the push-button, smoothly. Eject the tip by pressing firmly on the tip-ejector button. 6 - generaL guIdeLInes For good PIPettIng Make sure that you op- Table - Immersion Depth erate the push-button and Wait Time slowly and smoothly. Model Immersion Wait Time Depth (mm) -
Page 10: Accessories
7 - accessorIes To make pipetting more comfortable and more secure, Gilson has developed several accessories: To avoid the possibility of liquid running back into the pipette, store the pipette vertically. CaRROUseL™ Pipette stand (7 pipettes) f161401 TRIO™... -
Page 11: Glp Features
Refer to Chapter 12 - Cleaning and Decontamination. Please use the included safety bag to return the pipette to your local Gilson Service Center. The following table may help you to identify and correct the problem you might encounter. -
Page 12: Leak Test
* Not applicable for multichannel models However, if you can’t solve the problem, contact your Gilson representative. 10 - Leak test This test may be performed at any time to check that the pipette does not leak, especially after performing a maintenance or decontamination procedure. -
Page 13: Maintenance
Chapter 12 - Cleaning and Decontamination or to replacing the push-button, connecting nut, tip-ejector, tip-holder, seal and O-ring. PIPETmAN Neo P2N and P10N should not be disassembled, so you may only replace the push-button, tip-ejector, dual position tip-ejector and its adapter. With these pipettes if the tip-holder is damaged, the piston may also be damaged. - Page 14 Servicing the Piston Assembly you may remove the piston assembly for cleaning pur- poses only. If the piston assembly is changed, the pipette must be adjusted and calibrated in a Gilson authorized Service Center. Remove the tip-ejector (see above). Unscrew the connecting nut by turning it counter- clockwise.
- Page 15 Color coded push-button Connecting nut Piston assembly Seal O-ring Tip-ejector Tip-holder The piston assembly must not be autoclaved.
- Page 16 Reassemble the pipette (refer to the figure, page 15). Tighten the connecting nut (turn clockwise). Refit the tip-ejector (see above). changing the o-ring The O-ring and seal are on the piston; they must not be autoclaved, if worn or damaged in any way (chemical or mechanical), they must be replaced.
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Page 17: Cleaning And Decontamination
P10N contain miniaturized parts, it is best not to disas- semble these pipettes yourself; please contact your local Gilson authorized Service Center. You may refer to the decontamination procedure available on the Gilson website (www. gilson. com). Liquid must never enter the upper part (handle) of any pipette. cleaning The pipette must be cleaned, as described below, before it is decontaminated. - Page 18 50°C) or with a soft-cloth and brushes. Small round brushes with soft plastic bristles may be used to clean the interior of the tip-holder. Rinse the individual components with distilled water. Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue.
- Page 19 Check that the parts are dry before re-assembling the pipette. Set the pipette aside to stabilize at room temperature. Multichannel models The complete lower part can be autoclaved (20 minutes at 121°C, 0.1 MPa). The lower part has to be removed from the body. To remove: keep the tip-ejector button depressed,...
- Page 20 To fit the lower part on the body, Press on the push-button. Insert the lower part into the ejection rod. Release the push-button. Screw the connecting nut turning clockwise. Press on the tip-ejector button. Insert the ejector clip and release the tip-ejector button.
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Page 21: Specifications
figures are guaranteed only when genuine PIPETMAN DIAMOND Tips are used. Each pipette is inspected and validated by qualified technicians in accordance with the Gilson Quality System. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard, by type testing. - Page 22 Permissible errors Single channel Maximum Permissible Errors Model Volume Gilson ISO 8655 (Reference) (µL) systematic Random systematic Random error (µL) error (µL) error (µL) error (µL) ± 0.024 0.012 ± 0.08 0.04 (f144561) ± 0.025 0.012 ±...
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Page 23: Spare Parts
14 - sPare Parts single channel models Service kit 1st level includes: - 3 piston seals - 3 O-rings - 1 tip-holder Service kit 2nd level includes: - 1 push-button - 1 connecting nut - 1 tip-ejector for the P2N and P10N models only: - 1 tip-ejector and 1 adapter... - Page 24 multichannel models Tip-ejector (8 channels) Tip-ejector (12 channels) Ejector lock Ejector spacer (8 channels) Ejector spacer (12 channels)
- Page 25 P2n (F144561) and P10n (F144562) Description P10N C+D+E Service Kit 1st level F144501 F144502 A+B+F1+F2 Service Kit 2nd level F161970 F161971 Seal + O-ring (5 sets) F144861 F144862 Tip-ejector Adapter F144879 F144879 P20n (F144563) and P100n (F144564) Description P20N P100N C+D+E Service Kit 1st level F144495...
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Page 26: Ec Declaration Of Conformity
The company, gILson s.a.s. 19, avenue des Entrepreneurs BP. 145 - 95400 Villiers-le-Bel (France) Tel.: +33(0) 1 34 29 50 00 Fax: +33(0) 1 34 29 50 20 www.gilson.com Hereby certifies on its sole responsibility that the products listed below: PIPetman neo®... -
Page 27: Warranty
Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes. - Page 28 LT801521/D - ©2012 Gilson SAS All rights reserved July 2012English Printed in France Specifications subject to change without notifications - errors omitted. www.gilson.com sales@gilson.com service@gilson.com training@gilson.com gilson, Inc. World headquarters 3000 Parmenter Street P.O. Box 620027 Middleton, WI 53562-0027, USA...
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