Gilson PIPETMAN M User Manual
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Gilson PIPETMAN M User Manual

Electronic pipette
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  • Page 1 User’s Guide...

  • Page 2: Table Of Contents

    TABLE OF CONTENTS INTRODUCTION | 4 PARTS CHECKLIST AND ACCESSORIES | 5 Parts Checklist | 5 Accessories | 5 GETTING STARTED | 5 DESCRIPTION | 6 Reset | 6 Switch Off | 6 Display | 6 PIPETTING JUST LIKE WITH PIPETMAN | 7 Switch On | 7 Adjust the Volume Setting | 7 Purge Volume | 7...
  • Page 3 REPLACEMENT PARTS | 32 Single Channel Models | 32 Multichannel Models | 32 PIPETMAN M SOFTWARE What is PIPETMAN M Software? | 33 Passwords and User Information | 33 Home Screen | 34 Installing PIPETMAN M Software | 35 Uninstalling PIPETMAN M Software | 36...

  • Page 4: Introduction

    Combining PIPETMAN’s renowned accuracy, precision, and robustness with user-friendly functions, PIPETMAN M reduces pipetting fatigue, and increase pipetting efficiency. PIPETMAN M is a unique pipette with only two buttons to operate the pipette and reach all menu options: ●...

  • Page 5: Parts Checklist And Accessories

    PIPETMAN M, we strongly recommend that you to fully charge the NOTE battery. PIPETMAN M charges 80% of its full battery capacity in less than an hour, it takes three hours to fully charge the battery. Please refer to...

  • Page 6: Description

    Display The PIPETMAN M display is an Organic Light-Emitting Diode (OLED) screen. It shows the current mode and operation step, pipetting volume, battery indicator and piston status (aspirated and dispensed volume) in real time.Warning messages appear in the place of volume.

  • Page 7: Pipetting Just Like With Pipetman

    Chapter 6 Chapter 6 PIPETTING MODES PIPETMAN M offers more pipette modes for a large number of applications: you will find all pipetting modes in the menu (for system settings refer to the Chapter 7 Pipetting Speed Control on page 13 and Chapter 13 Configuration on page 20).

  • Page 8: Repetitive Mode

    Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use PIPETMAN DIAMOND Tips for assurance of accurate and precise results, refer to PIPETMAN DIAMOND Tips Chapter 8 on page 13). NOTICE Always fit a tip before using any pipette.

  • Page 9: Mix Mode

    Press the push button. DISCARD appears on the screen. Discard the extra volume andcontinue dispensing the aliquots. PIPETMAN M distributes the aliquot volume each time you click the push button. The number of aliquots left to dispense is displayed on the screen.
  • Page 10 If you set the VOLUME 3 = 0 µL, then the cycle will stop after the MIX NOTE step. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use PIPETMAN DIAMOND Tips for assurance of accurate and precise results; refer to Chapter 8 PIPETMAN DIAMOND Tips on page 13).

  • Page 11: Reverse Mode

    Press the push button, set the volume by turning the push button and press again to confirm your settings. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use PIPETMAN DIAMOND Tips for assurance of accurate and precise results; refer to...

  • Page 12: Custom Mode

    If PIPETMAN M Software is not launched automatically, then double-click the PIPETMAN M icon on the desktop. Once installed, the PIPETMAN M Software can be run by any user on the installed PC. You can then create, edit, import, or export custom protocols easily and rapidly.

  • Page 13: Pipetting Speed Control

    Chapter 7 PIPETTING SPEED CONTROL PIPETMAN M is set by default on speed 6 (maximum speed). You may need to change the speed of aspiration or dispensing depending on your application. You can change aspiration speed and dispensing speed independently of one other: from very slow to very fast (speed 1 —...
  • Page 14 20 minutes at 0.1 MPa. NOTICE Do not autoclave PIPETMAN Filter Tips as the filter will be damaged. To ensure the best performance from your Gilson pipette, you shoud ALWAYS use PIPETMAN DIAMOND Tips (in accordance with NOTE ISO8655) system, because PIPETMAN Tips were used to establish the specifications.

  • Page 15: Guidelines For Good Pipetting

    MultiChannel Model PIPETMAN DIAMOND tips are best fitted with the ROCKY RACK technique, invented by Gilson, available only in our TIPACK and TOWERPACK. Pre-Wet the Tips All Models Pre-wetting the tips before pipetting helps prepare the tips for the best pipetting performance.

  • Page 16: General Guidelines For Good Pipetting

    Pipette Holder to store your PIPETMAN M pipette in the vertical position. Alternatively the POWER CARROUSEL can be used to store and charge up to five PIPETMAN M pipettes. ● When pipetting liquids with temperatures different to the ambient temperature, pre-we the tip several times before use in order to reach equilibrium between the temperatures of the liquid and the pipette’s dead volume.

  • Page 17: Personal Label - Name And/Or Application Tag

    Clamp the clip back in place. Chapter 11 GOOD LABORATORY PRACTICE FEATURES Your PIPETMAN M is fully compliant to ISO8655 standard and is CE marked (for EMC and LDV directives). PIPETMAN M includes the following good laboratory practice (GLP) features.

  • Page 18: Alarms

    POWER MANAGEMENT The PIPETMAN M battery has been USB port designed to ensure 900 pipetting cycles (750 for PIPETMAN M Multi 200 µL and Plug 300 µL) at maximum speed (up to nine 96-well plates) between two charge cycles. For service continuity when needing more...

  • Page 19: Working With Charger Connected To The Pipette

    Plug the power supply into your pipette. Press the push button to start your pipette. PIPETMAN M is ready to pipette. You can also recharge PIPETMAN M by USB with your PC; however it will NOTE take much longer than by power supply.

  • Page 20: Configuration

    Press and hold the Mode button for two seconds to confirm your selection. Chapter 14 MAINTENANCE PIPETMAN M requires very little maintenance. However, to ensure pipette accuracy, precision, and robustness please proceed periodically with a two–minute inspection as recommended by Gilson.

  • Page 21: Service Information

    Cleaning and Decontamination on page 26). Service Information PIPETMAN M provides you with all required service information. It will help you establish an easy diagnosis and plan any service operation with your accredited Gilson service center. You will find in the SERVICE menu following sub menus: ●...

  • Page 22: Adjustment

    To return to the factory settings, choose Reset settings. Your PIPETMAN M will be reset to the three factory calibration values that are permanently stored in the pipette’s firmware. In accordance with ISO 8655 Gilson recommends a gravimetric procedure for pipette calibration.

  • Page 23: Maintenance Warning

    Maintenance Warning PIPETMAN M notifies you when maintenance is due (Service alert is set by default to “On” ). The following message will appear: After validation by a click, the following message appears: If you click to confirm within 10 seconds. the pipette will automatically switch to Disassembly mode so you can carry out your maintenance safely.
  • Page 24 O-ring Changing the Seal and/or O-ring The O-ring is positioned on the piston; it should not be autoclaved, if worn or damaged in any way, it must be replaced Replacement Parts (refer to Chapter 20 on page 32). To access the O-ring, remove the tip ejector and unscrew the Figure 16 lower part of the tip holder.

  • Page 25: Maintenance Operation For Multichannel Models

    How to Lubricate the Piston (except P10M) Use only Gilson lubricant (P/N: F2070902, as supplied). Squeeze a small quantity from the tube onto a clean, lintless cloth. Use the cloth to transfer the lubricant to the piston.

  • Page 26: Cleaning And Decontamination

    (Polyvinylidene Fluoride), PEI (Polyetherimide), and stainless steel. Cleaning The pipette must be cleaned, as described below, before it is decontaminated. For cleaning your PIPETMAN M you may use a simple soap solution or any of the solutions mentioned in Decontamination Procedures for Gilson Pipettes.

  • Page 27: Multichannel Models

    Leave the parts to dry by evaporation or wipe them with a clean, soft cloth or lint-free tissue. Refit the tip ejector. Please note that although the lower part of PIPETMAN M multichannel can NOTE withstand a few autoclaving cycles, we do not recommend autoclaving it.

  • Page 28: Leak Test

    ● Multichannel models a tip ejector, ejector locks, and ejector spacer. Please note that although the lower part of PIPETMAN M multichannel can withstand a few number of autoclaving cycles, we do not recommend autoclaving it. Disassemble the pipette as described in Chapter 14 Maintenance on page 20).

  • Page 29: Troubleshooting

    Software needs to be reset Chapter 18 SPECIFICATIONS PIPETMAN M is a high quality pipette that offers excellent accuracy and precision; it is fully compliant with ISO 8655 and is CE marked. The figures given in the PIPETMAN M Maximum Permissible Errors Table 2 on page 30 were obtained using “PIPETMAN DIAMOND Tips”.
  • Page 30 ≤ 1.8 ± 32 ≤ 12 1200 Gilson maximum permissible errors are guaranteed only when PIPETMAN pipettes are used with the recommended PIPETMAN DIAMOND Tips. Under these conditions: ● Gilson volumetric specifications in standard pipetting (PIPET mode) are guaranteed with a performance exceeding ISO 8655-2 recommendations for this mode.

  • Page 31: Safety Precautions And Limitations Of Use

    ● Use only genuine PIPETMAN DIAMOND Tips and original Gilson’s accessories and spare parts. If the equipment is used in a manner not specified by Gilson in the user’s guide, the protection provided in the equipment may be impaired. ●...

  • Page 32: Replacement Parts

    Chapter 20 REPLACEMENT PARTS Single Channel Models DESCRIPTION P10M P20M P100M P200M P300M P1200M P5000M P10mLM Connecting nut F807012 F807012 F807012 F807012 F807012 F807012 F807012 F807012 Battery tag, 1x4 F807013 F807013 F807013 F807013 F807013 F807013 F807013 F807013 Ejector tag, 1x4 F807014 F807014 F807014...

  • Page 33: Pipetman M Software

    PIPETMAN® M Software is an easy-to-use software for creating custom pipetting protocols for PIPETMAN M pipettes. Use it to create personalized pipetting protocols on your PC, and to transfer these protocols to PIPETMAN M pipettes in the Custom Mode, where you can execute them.

  • Page 34: Home Screen

    In the case of a forgotten password, click the Forgotten Password button on the PIPETMAN M login window. Select one of the following options to recover the forgotten password ● I know the answer to the hidden question ● I have the recovery password ●...

  • Page 35: Installing Pipetman M Software

    PC if Create Desktop Shortcut is checked. If PIPETMAN M Software is not launched automatically, double-click the PIPETMAN M icon on the desktop. Once installed, the PIPETMAN M Software can be run by any user on the installed PIPETMAN® M USER’S GUIDE...

  • Page 36: Uninstalling Pipetman M Software

    Protocol info and Protocol setup tabs. ● In the Protocol info tab, select a pipette from a list of PIPETMAN M models. Enter the name of the user who created the protocol (protocol created by: field) and enter any comments into the Comments box, if required.
  • Page 37 20 µL and the number of iterations is set to 3, the tasks will be executed on PIPETMAN M in the following order: 1. Aspirate 20 µL, 2. Dispense 20 µL, 3. Aspirate 20 µL, 4. Dispense 20 µL, 5. Aspirate 20 µL, and 6.
  • Page 38 ● Comments: enter any comments related to the task. For certain PIPETMAN M models the minimal volumes in Aspirate and NOTE Dispense tasks might be different if these tasks are located inside Repeat loop.
  • Page 39 task has the following parameters: ● Task name : specify the name of the task. ● Volume (µL) : specify the volume for the task in µL. ● Aspiration speed : specify the speed for the aspiration. ● Dispense speed : specify the speed for the dispense.
  • Page 40 Figure 23 pipette volume indicator will be surrounded with a Numbers displayed next to red rectangle. colored bars represents volume remaining in the pipette at the end of protocol (displayed in PIPETMAN M SOFTWARE PIPETMAN® M microliters).
  • Page 41 Warning and Error Messages PIPETMAN M Software verifies the protocol after the addition of each task, or after modification of a task, to determine whether the volume of the pipette is still within the volume specifications for that particular PIPETMAN M model. Should the volume...

  • Page 42: Transferring Custom Protocols To The Pipette

    PIPETMAN M model. Transferring Custom Protocols to the Pipette To transfer a protocol to a PIPETMAN M pipette, click the Transfer custom protocols to pipette button and follow the instructions on the screen: Connect pipette to the PC via USB cable and click Next.
  • Page 43 ● You can transfer a protocol created for a different PIPETMAN M model to the connected pipette, providing the protocol is using volumes within the connected pipette’s volume specifications. Protocols that contain volumes outside connected pipette’s volume will have a red error sign ●...

  • Page 44: Updating Pipette Settings

    Updating Pipette Settings Here you can set the following configurations on the connected PIPETMAN M pipette: ● Set the Beep to On or Off (switches the tone of the pipette to On or Off). Beep On and Off only affects the built-in pipette protocols, as well as Aspirate , Dispense, Mix , Purge tasks (but NOT beep task!).

  • Page 45: Updating Pipette Firmware

    Updating Pipette Firmware To update firmware on PIPETMAN M pipette follow these steps: Click Update pipette settings in the main menu. If a pipette is already correctly connected, proceed to step 4.

  • Page 46: Other Administration

    Manage the information related to your Institution/ Company. Pipette Management Here you can register your individual PIPETMAN M pipettes. Registering a pipette is required to allow for transfer of your personalized protocols onto the pipette, as well as updating the pipette settings.
  • Page 47 View History Here you can view the history of protocol uploads to PIPETMAN M pipettes ( tab Protocol upload ) and history of protocol modifications (tab Protocol management ). To display the history of protocol uploads to PIPETMAN M pipettes, navigate to the Protocol upload tab , select the time interval and click Show history .
  • Page 48 Enter the information related to your Institution/Company here or modify it. Simply enter the information in provided fields and click Save . None of the information is mandatory. Figure 30 Enter the information related to your Institution/ Company PIPETMAN M SOFTWARE PIPETMAN® M...

  • Page 49: Ec Declaration Of Conformity

    Chapter 22 EC DECLARATION OF CONFORMITY Gilson certifies on its sole responsibility that the products listed below: P10M, P20M, P100M, P200M, P300M, P1200M, P5000M, P10mLM, P8x10M, P12x10M, P8x20M, P12x20M, P8x100M, P12x100M, P8x200M, P12x200M, P8x300M, P12x300M, P8x1200M, P12x1200M. Comply with the requirements of the following standards:...

  • Page 50: Warranty

    This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes.

  • Page 51: Appendices

    APPENDICES Appendix A – Example of a Performance Check Below is an example of how to evaluate the performance of PIPETMAN P10M at 1 µL. Calculate the mean volume. Determine the mean value e For a temperature of 21.5°C and an air of the evaporation loss e that pressure of 1013 hPa, the Z factor is...

  • Page 52: Appendix B - Z Factor

    Appendix B – Z Factor The reference calculation equation is : Z = [1/(P )] [1-(P Where: = density of air at t°C. = density of the test liquid at t°C. = density of the balance weights. Use 8 g/cc for P Weights conforming to International recommendation N°33 of OIML have NOTE been adjusted to give results when weighing in air as if the density of the...

  • Page 53: Appendix C - Evaporation Loss

    Appendix C – Evaporation Loss Procedure for the Determination of Evaporation Loss Use the same distilled water, weighing vessel, and balance as you will be using for the gravimetric check. Half fill the weighing vessel with distilled water. Cover the weighing vessel with its lid and place it on the balance using a pair of tweezers.
  • Page 54 LT801563/D | ©2021 Gilson, Inc. All rights reserved.

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