Troubleshooting - Nanolive 3D Cell Explorer-fluo User Manual

11. Troubleshooting

Calibration
Calibration failed err.1.1
If applicable, reduce the quantity of liquid above your sample (-> Check our Sample Preparation Protocol in Section 5). Check that no opaque object
can intersect the laser beam while the arm is rotating. If using a dish, check that you are imaging close to its center such that the laser beam cannot
intersect the wall of the dish instead of the top.
Calibration failed err.1.4
Sample may be too absorbent. Try to move in a different area and re-do the acquisition. If the error persists, please get in touch with our customer
service.
Acquisition
Acquisition failure err.2.1
If applicable, try to move to another position within your sample. If the error persists try to clean the microscope objective and check that you com-
ply with the sample preparation protocol ( -> Check our Sample Preparation Protocol).
Connection
Connection failure err.3.1
If STEVE cannot establish communication with the 3D Cell Explorer, please verify that the backside USB 2.0 is correctly plugged in to the 3D Cell
Explorer and to your computer, that the microscope has electrical power and that it is switched on. If the error persists, unplug all cables, reboot
your computer and plug the cables back (check Start-up - Section 4).
Connection failure err.3.2
If STEVE cannot establish communication with the 3D Cell Explorer, please verify that the USB 3.0 cable coming from the camera is correctly
screwed in and that it is properly connected to your computer's USB 3.0 port.
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