Nanolive 3D Cell Explorer-fluo User Manual page 49

10. Software STEVE
Time-lapse acquisitions
How to do a time-lapse acquisition
In a time-lapse acquisition, multiple 3D reconstructions in a range of time are attained.
To perform a time lapse, click on the 4D button or on Time Lapse in the Microscope menu. The mounting medium refractive index selection dialog
will appear (see section "How to select the mounting medium's refractive index" previously explained). Once a refractive index is selected, a new
dialog will open to specify the speed and the duration of the acquisition (Figure 35 green frame).
Figure 35 time lapse setting window
The time lapse can be performed either at the maximum speed or by setting the time interval between two consecutive frames.
The duration of the time lapse acquisition can be determined either by stopping it manually (by clicking on the Stop Acquiring button) or by setting
the ending time for it to stop automatically.
At the end of the acquisition, STEVE will enable to digitally stain the data and interactively explore the cell and its subcomponents. Further down,
we explain the exact steps on how to achieve this. Please refer to "How to digitally stain cells with STEVE".
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