Nanolive 3D Cell Explorer-fluo User Manual page 50

10. Software STEVE
Fluorescence time-lapse acquisition
Acquiring fluorescence time-lapses
To perform a time lapse, click on the 4D button or on Time Lapse in the Microscope menu. A selection dialog will appear to specify the speed and
the duration of the acquisition, which can be individually set up (Figure 36). Once the settings for the time lapse, the refractive index and the fluo-
rescence are selected, a new dialogue will appear to adjust the fluorescence channel parameters (Figure 37). Adjust the fluorescence parameters as
per your needs (Figure xy9). Then click on „ok". Your acquisition will start.
Please note: in case of maximal speed during a time-lapse acquisition, the fluorescence channels are disabled!
Figure 36 Time lapse setting window
Figure 37 Dialogue window fluorescence
parameters
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